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Non-contiguous-Finished Genome Sequence and Description of Paenibacillus camerounensis sp. nov.

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Abstract

Strain G4T was isolated from the stool sample of a wild gorilla (Gorilla gorilla gorilla) from Cameroon. It is a facultative anaerobic, Gram-negative, rod-shaped bacterium. This strain exhibits a 16S rRNA nucleotide sequence similarity of 97.48 % with Paenibacillus typhae, the phylogenetically closest species with standing nomenclature. Moreover, the strain G4T presents some phenotypic differences when compared to other Paenibacillus species and shows a low MALDI-TOF Mass Spectrometry score that does not allow any identification. Thus, it is likely that this strain represents a new species. Here, we describe the characteristics of this organism, complete genome sequence, and annotation. The 6,933,847 bp size genome (1 chromosome but no plasmid) contains 5972 protein-coding genes and 54 RNAs genes, including 44 tRNA genes. In addition, digital DNA-DNA hybridization values for the genome of the strain G4T against the closest Paenibacillus genomes range between 19.7 and 22.1, once again confirming its new status as a new species. On the basis of these polyphasic data, consisting of phenotypic and genomic analyses, we propose the creation of Paenibacillus camerounensis sp. nov. that contains the strain G4T.

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Abbreviations

URMITE:

Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes

CSUR:

Collection de Souches de l’Unité des Rickettsies

DSM:

Deutsche Sammlung von Mikroorganismen

MALDI-TOF MS:

Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry

TE buffer:

Tris-EDTA buffer

GGDC:

Genome-to-Genome Distance Calculator

dDDH:

Digital DNA-DNA hybridization

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Acknowledgments

Fadi Bittar was supported by a Chair of Excellence IRD provided by the Institut de Recherche pour le Développement / Méditerranée-Infection foundation. Mamadou Bhoye Keita was funded by the Méditerranée-Infection foundation. The authors thank the Xegen company for automating the genome annotation process.

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Correspondence to Fadi Bittar.

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Keita, M.B., Padhmanabhan, R., Robert, C. et al. Non-contiguous-Finished Genome Sequence and Description of Paenibacillus camerounensis sp. nov.. Microb Ecol 71, 990–998 (2016). https://doi.org/10.1007/s00248-015-0722-4

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  • DOI: https://doi.org/10.1007/s00248-015-0722-4

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