Microbial Ecology

, Volume 66, Issue 2, pp 462–470

Dysbiosis Signature of Fecal Microbiota in Colorectal Cancer Patients

Authors

  • Na Wu
    • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of MicrobiologyChinese Academy of Sciences
  • Xi Yang
    • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of MicrobiologyChinese Academy of Sciences
  • Ruifen Zhang
    • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of MicrobiologyChinese Academy of Sciences
  • Jun Li
    • Department of Abdominal Surgical Oncology, Cancer Institute and HospitalChinese Academy of Medical Sciences
  • Xue Xiao
    • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of MicrobiologyChinese Academy of Sciences
  • Yongfei Hu
    • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of MicrobiologyChinese Academy of Sciences
  • Yanfei Chen
    • State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated HospitalZhejiang University
  • Fengling Yang
    • State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated HospitalZhejiang University
  • Na Lu
    • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of MicrobiologyChinese Academy of Sciences
  • Zhiyun Wang
    • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of MicrobiologyChinese Academy of Sciences
  • Chunguang Luan
    • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of MicrobiologyChinese Academy of Sciences
  • Yulan Liu
    • Department of GastroenterologyPeking University People’s Hospital
  • Baohong Wang
    • State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated HospitalZhejiang University
  • Charlie Xiang
    • State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated HospitalZhejiang University
  • Yuezhu Wang
    • Chinese National Human Genome Center at Shanghai
  • Fangqing Zhao
    • Beijing Institutes of Life ScienceChinese Academy of Sciences
  • George F. Gao
    • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of MicrobiologyChinese Academy of Sciences
  • Shengyue Wang
    • Chinese National Human Genome Center at Shanghai
  • Lanjuan Li
    • State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated HospitalZhejiang University
    • Department of Abdominal Surgical Oncology, Cancer Institute and HospitalChinese Academy of Medical Sciences
    • CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of MicrobiologyChinese Academy of Sciences
Host Microbe Interactions

DOI: 10.1007/s00248-013-0245-9

Cite this article as:
Wu, N., Yang, X., Zhang, R. et al. Microb Ecol (2013) 66: 462. doi:10.1007/s00248-013-0245-9

Abstract

The human gut microbiota is a complex system that is essential to the health of the host. Increasing evidence suggests that the gut microbiota may play an important role in the pathogenesis of colorectal cancer (CRC). In this study, we used pyrosequencing of the 16S rRNA gene V3 region to characterize the fecal microbiota of 19 patients with CRC and 20 healthy control subjects. The results revealed striking differences in fecal microbial population patterns between these two groups. Partial least-squares discriminant analysis showed that 17 phylotypes closely related to Bacteroides were enriched in the gut microbiota of CRC patients, whereas nine operational taxonomic units, represented by the butyrate-producing genera Faecalibacterium and Roseburia, were significantly less abundant. A positive correlation was observed between the abundance of Bacteroides species and CRC disease status (R = 0.462, P = 0.046 < 0.5). In addition, 16 genera were significantly more abundant in CRC samples than in controls, including potentially pathogenic Fusobacterium and Campylobacter species at genus level. The dysbiosis of fecal microbiota, characterized by the enrichment of potential pathogens and the decrease in butyrate-producing members, may therefore represent a specific microbial signature of CRC. A greater understanding of the dynamics of the fecal microbiota may assist in the development of novel fecal microbiome-related diagnostic tools for CRC.

Supplementary material

248_2013_245_MOESM1_ESM.pdf (159 kb)
ESM 1 (PDF 159 kb)

Copyright information

© Springer Science+Business Media New York 2013