Host Microbe Interactions

Microbial Ecology

, Volume 66, Issue 2, pp 462-470

Dysbiosis Signature of Fecal Microbiota in Colorectal Cancer Patients

  • Na WuAffiliated withCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
  • , Xi YangAffiliated withCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
  • , Ruifen ZhangAffiliated withCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
  • , Jun LiAffiliated withDepartment of Abdominal Surgical Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences
  • , Xue XiaoAffiliated withCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
  • , Yongfei HuAffiliated withCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
  • , Yanfei ChenAffiliated withState Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, Zhejiang University
  • , Fengling YangAffiliated withState Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, Zhejiang University
  • , Na LuAffiliated withCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
    • , Zhiyun WangAffiliated withCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
    • , Chunguang LuanAffiliated withCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
    • , Yulan LiuAffiliated withDepartment of Gastroenterology, Peking University People’s Hospital
    • , Baohong WangAffiliated withState Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, Zhejiang University
    • , Charlie XiangAffiliated withState Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, Zhejiang University
    • , Yuezhu WangAffiliated withChinese National Human Genome Center at Shanghai
    • , Fangqing ZhaoAffiliated withBeijing Institutes of Life Science, Chinese Academy of Sciences
    • , George F. GaoAffiliated withCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
    • , Shengyue WangAffiliated withChinese National Human Genome Center at Shanghai
    • , Lanjuan LiAffiliated withState Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, Zhejiang University
    • , Haizeng ZhangAffiliated withDepartment of Abdominal Surgical Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences Email author 
    • , Baoli ZhuAffiliated withCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences Email author 

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Abstract

The human gut microbiota is a complex system that is essential to the health of the host. Increasing evidence suggests that the gut microbiota may play an important role in the pathogenesis of colorectal cancer (CRC). In this study, we used pyrosequencing of the 16S rRNA gene V3 region to characterize the fecal microbiota of 19 patients with CRC and 20 healthy control subjects. The results revealed striking differences in fecal microbial population patterns between these two groups. Partial least-squares discriminant analysis showed that 17 phylotypes closely related to Bacteroides were enriched in the gut microbiota of CRC patients, whereas nine operational taxonomic units, represented by the butyrate-producing genera Faecalibacterium and Roseburia, were significantly less abundant. A positive correlation was observed between the abundance of Bacteroides species and CRC disease status (R = 0.462, P = 0.046 < 0.5). In addition, 16 genera were significantly more abundant in CRC samples than in controls, including potentially pathogenic Fusobacterium and Campylobacter species at genus level. The dysbiosis of fecal microbiota, characterized by the enrichment of potential pathogens and the decrease in butyrate-producing members, may therefore represent a specific microbial signature of CRC. A greater understanding of the dynamics of the fecal microbiota may assist in the development of novel fecal microbiome-related diagnostic tools for CRC.