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The Interactions of Vibrio vulnificus and the Oyster Crassostrea virginica

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Abstract

The human bacterial pathogen, Vibrio vulnificus, is found in brackish waters and is concentrated by filter-feeding molluscan shellfish, especially oysters, which inhabit those waters. Ingestion of raw or undercooked oysters containing virulent strains of V. vulnificus can result in rapid septicemia and death in 50 % of victims. This review summarizes the current knowledge of the environmental interactions between these two organisms, including the effects of salinity and temperature on colonization, uptake, and depuration rates of various phenotypes and genotypes of the bacterium, and host–microbe immunological interactions.

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References

  1. Johnston JM, Becker SF, McFarland LM (1985) Gastroenteritis in patients with stool isolates of Vibrio vulnificus. Am J Med 80(2):336–338

    Article  Google Scholar 

  2. Oliver JD (1989) Vibrio vulnificus. In: Doyle MP (ed) Foodborne bacterial pathogens. Marcel Dekker, Inc., New York, pp 569–599

    Google Scholar 

  3. Oliver JD (2006) Vibrio vulnificus. In: Thompson FL, Austin B, Swings J (eds) The biology of vibrios. American Society of Microbiology, Washington, pp 349–366

    Google Scholar 

  4. Jones MK, Oliver JD (2009) Vibrio vulnificus: disease and pathogenesis. Infect Immun 77(5):1723–1733

    Article  PubMed  CAS  Google Scholar 

  5. Oliver J (2005) Wound infections caused by Vibrio vulnificus and other marine bacteria. Epidemiol Infect 133(03):383–391

    Article  PubMed  CAS  Google Scholar 

  6. Mead PS, Slusker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, Griffin PM, Tauxe RBV (1999) Food-related illness and death in the United States. Emerg Infect Dis 5:607–625

    Article  PubMed  CAS  Google Scholar 

  7. Bisharat N, Agmon V, Finkelstein R, Raz R, Ben-Dror G, Lerner L, Soboh S, Colodner R, Cameron DN, Wykstra DL (1999) Clinical, epidemiological, and microbiological features of Vibrio vulnificus biogroup 3 causing outbreaks of wound infection and bacteraemia in Israel. Israel Vibrio Study Group. Lancet 354(9188):1421–1424

    Article  PubMed  CAS  Google Scholar 

  8. Amaro C, Biosca EG (1996) Vibrio vulnificus biotype 2, pathogenic for eels, is also an opportunistic pathogen for humans. Appl Environ Microbiol 62(4):1454

    PubMed  CAS  Google Scholar 

  9. Tison DL, Nishibuchi M, Greenwood JD, Seidler RJ (1982) Vibrio vulnificus biogroup 2: new biogroup pathogenic for eels. Appl Environ Microbiol 44(3):640–646

    PubMed  CAS  Google Scholar 

  10. Warner JM, Oliver JD (1999) Randomly amplified polymorphic DNA analysis of clinical and environmental isolates of Vibrio vulnificus and other Vibrio species. Appl Environ Microbiol 65:526–534

    Google Scholar 

  11. Rosche T, Yano Y, Oliver JD (2005) A rapid and simple PCR analysis indicates there are two subgroups of Vibrio vulnificus which correlate with clinical and environmental isolation. Microbiol Immunol 49(4):381–389

    PubMed  CAS  Google Scholar 

  12. Warner JM, Oliver JD (1999) Randomly amplified polymorphic DNA analysis of clinical and environmental isolates of Vibrio vulnificus and other Vibrio species. Appl Environ Microbiol 65:1141–1144

    PubMed  CAS  Google Scholar 

  13. Nilsson WB, Paranjype RN, DePaola A, Strom MS (2003) Sequence polymorphism of the 16S rRNA gene of Vibrio vulnificus is a possible indicator of strain virulence. J Clin Microbiol 41(1):442–446

    Article  PubMed  CAS  Google Scholar 

  14. Park JH, Cho Y-J, Chun J, Seok Y-J, Lee JK, Kim K-S, Lee K-H, Park S-J, Choi SH (2001) Complete genome sequence of Vibrio vulnificus MO6-24/O. J Bacteriol 193(8):2062–2063

    Article  Google Scholar 

  15. Kim YR, Lee SE, Kim CM, Kim SY, Shin EK, Shin DH, Chung SS, Choy HE, Progulske-Fox A, Hillman JD, Handfield M, Rhee JH (2003) Characterization and pathogenic significance of Vibrio vulnificus antigens preferentially expressed in septicemic patients. Infect Immun 71(10):5461–5471

    Article  PubMed  CAS  Google Scholar 

  16. Froelich BA, Williams TC, Noble RT, Oliver JD (2012) Apparent loss of Vibrio vulnificus from North Carolina oysters coincides with a drought-induced increase in salinity. Appl Environ Microbiol 78(11):3885–3889. doi:10.1128/aem.07855-11

    Article  PubMed  CAS  Google Scholar 

  17. Gulig P, Vd C-L, Wright A, Walts B, Telonis-Scott M, McIntyre L (2010) SOLiD sequencing of four Vibrio vulnificus genomes enables comparative genomic analysis and identification of candidate clade-specific virulence genes. BMC Genomics 11(1):512

    Article  PubMed  Google Scholar 

  18. Morrison SS, Williams T, Cain A, Froelich B, Taylor C, Baker-Austin C, Verner-Jeffreys D, Hartnell R, Oliver JD, Gibas CJ (2012) Pyrosequencing-based comparative genome analysis of Vibrio vulnificus environmental isolates. PLoS One 7(5):e37553. doi:10.1371/journal.pone.0037553

    Article  PubMed  CAS  Google Scholar 

  19. Stanley JG, Sessers MA (1986) Species profiles: life histories of environmental requirements of coastal fishes and invertebrates (Gulf of Mexico)—American oyster. US Fish Wildl Serv Biol Rep 11.64:25

    Google Scholar 

  20. Shumway S (1996) Natural environmental factors. In: Kennedy VS, Newell RIE, Eble AF (eds) The Eastern oyster Crassostrea virginica. Maryland Sea Grant College, College Park, pp 467–513

    Google Scholar 

  21. Eastern Oyster Biological Review Team (2007) Status review of the Eastern oyster (Crassostrea virginica). Report to the National Marine Fisheries Service, Northeast Regional Office, Gloucester

  22. Oliver JD, Warner RA, Cleland DR (1983) Distribution of Vibrio vulnificus and other lactose-fermenting vibrios in the marine environment. Appl Environ Microbiol 45(3):985–998

    PubMed  CAS  Google Scholar 

  23. Warner EB, Oliver JD (2007) Population structures of two genotypes of Vibrio vulnificus in oysters (Crassostrea virginica) and seawater. Appl Environ Microbiol 74(1):80–85

    Article  PubMed  Google Scholar 

  24. Han F, Pu S, Hou A, Ge B (2009) Characterization of clinical and environmental types of Vibrio vulnificus isolates from Louisiana oysters. Foodborne Pathog Dis 6(10):1251–1258. doi:10.1089/fpd.2009.0343

    Article  PubMed  CAS  Google Scholar 

  25. Staley C, Jones MK, Wright AC, Harwood VJ (2011) Genetic and quantitative assessment of Vibrio vulnificus populations in oyster (Crassostrea virginica) tissues. Environ Microbiol Rep 3(5):543–549. doi:10.1111/j.1758-2229.2011.00256.x

    Article  CAS  Google Scholar 

  26. Warner E, Oliver JD (2008) Population structures of two genotypes of Vibrio vulnificus in oysters (Crassostrea virginica) and seawater. Appl Environ Microbiol 74(1):80–85. doi:10.1128/aem.01434-07

    Article  PubMed  CAS  Google Scholar 

  27. Vickery M, Nilsson W, Strom M, Nordstrom J, DePaola A (2007) A real-time PCR assay for the rapid determination of 16S rRNA genotype in Vibrio vulnificus. J Microbiol Methods 68:376–384

    Article  PubMed  CAS  Google Scholar 

  28. Lin M, Payne DA, Schwarz JR (2003) Intraspecific diversity of Vibrio vulnificus in Galveston Bay water and oysters as determined by randomly amplified polymorphic DNA PCR. Appl Environ Microbiol 69(6):3170–3175

    Article  PubMed  CAS  Google Scholar 

  29. Kirs M, DePaola A, Fyfe R, Jones JL, Krantz J, Van Laanen A, Cotton D, Castle M (2011) A survey of oysters (Crassostrea gigas) in New Zealand for Vibrio parahaemolyticus and Vibrio vulnificus. Int J Food Microbiol 147(2):149–153. doi:10.1016/j.ijfoodmicro.2011.03.012

    Article  PubMed  CAS  Google Scholar 

  30. Froelich B, Ringwood A, Sokolova I, Oliver J (2010) Uptake and depuration of the C- and E-genotypes of Vibrio vulnificus by the Eastern oyster (Crassostrea virginica). Environ Microbiol Rep 2(1):112–115. doi:10.1111/j.1758-2229.2009.00112.x

    Article  CAS  Google Scholar 

  31. Hoi L, Larsen JL, Dalsgaard I, Dalsgaard A (1998) Occurrence of Vibrio vulnificus biotypes in Danish marine environments. Appl Environ Microbiol 64(1):7–13

    PubMed  CAS  Google Scholar 

  32. Johnson CN, Flowers AR, Noriea NF, Zimmerman AM, Bowers JC, DePaola A, Grimes DJ (2010) Relationships between environmental factors and pathogenic vibrios in the Northern Gulf of Mexico. Appl Environ Microbiol 76(21):7076–7084. doi:10.1128/AEM.00697-10

    Article  PubMed  CAS  Google Scholar 

  33. Lin M, Schwarz JR (2003) Seasonal shifts in population structure of Vibrio vulnificus in an estuarine environment as revealed by partial 16S ribosomal DNA sequencing. FEMS Microbiol Ecol 45(1):23–27. doi:10.1016/s0168-6496(03)00091-6

    Article  PubMed  CAS  Google Scholar 

  34. Parvathi A, Kumar HS, Karunasagar I, Karunasagar I (2004) Detection and enumeration of Vibrio vulnificus in oysters from two estuaries along the southwest coast of India, using molecular methods. Appl Environ Microbiol 70(11):6909–6913. doi:10.1128/aem.70.11.6909-6913.2004

    Article  PubMed  CAS  Google Scholar 

  35. Tamplin M, Rodrick GE, Blake NJ, Cuba T (1982) Isolation and characterization of Vibrio vulnificus from two Florida estuaries. Appl Environ Microbiol 44(6):1466–1470

    PubMed  CAS  Google Scholar 

  36. Tilton RC, Ryan RW (1987) Clinical and ecological characteristics of Vibrio vulnificus in the Northeastern United States. Diagn Micr Infec Dis 6(2):109–117. doi:10.1016/0732-8893(87)90094-0

    Article  CAS  Google Scholar 

  37. Hlady WG (1997) Vibrio infections associated with raw oyster consumption in Florida, 1981–1994. J Food Protect 60:353–357

    Google Scholar 

  38. Shapiro RL, Altekruse S, Hutwagner L, Bishop R, Hammond R, Wilson S, Ray B, Thompson S, Tauxe RV, Griffin PM (1998) The role of gulf coast oysters harvested in warmer months in Vibrio vulnificus infections in the United States, 1988–1996. J Infect Dis 178(3):752–759. doi:10.1086/515367

    Article  PubMed  CAS  Google Scholar 

  39. Fukushima H, Seki R (2004) Ecology of Vibrio vulnificus and Vibrio parahaemolyticus in brackish environments of the Sada River in Shimane Prefecture, Japan. FEMS Microbiol Ecol 48(2):221–229. doi:10.1016/j.femsec.2004.01.009

    Article  PubMed  CAS  Google Scholar 

  40. Kaspar CW, Tamplin ML (1993) Effects of temperature and salinity on the survival of Vibrio vulnificus in seawater and shellfish. Appl Environ Microbiol 59(8):2425–2429

    PubMed  CAS  Google Scholar 

  41. Motes ML, DePaola A, Cook DW, Veazey JE, Hunsucker JC, Garthright WE, Blodgett RJ, Chirtel SJ (1998) Influence of water temperature and Salinity on Vibrio vulnificus in Northern Gulf and Atlantic coast oysters (Crassostrea virginica). Appl Environ Microbiol 64(4):1459–1465

    PubMed  CAS  Google Scholar 

  42. Randa MA, Polz MF, Lim E (2004) Effects of temperature and salinity on Vibrio vulnificus population dynamics as assessed by quantitative PCR. Appl Environ Microbiol 70(9):5469–5476. doi:10.1128/aem.70.9.5469-5476.2004

    Article  PubMed  CAS  Google Scholar 

  43. Vanoy RW, Tamplin ML, Schwarz JR (1992) Ecology of Vibrio vulnificus in Galveston bay oysters, suspended particulate matter, sediment and seawater: detection by monoclonal antibody-immunoassay-most probable number procedures. J Ind Microbiol Biot 9(3):219–223. doi:10.1007/bf01569626

    Google Scholar 

  44. Pfeffer CS, Hite MF, Oliver JD (2003) Ecology of Vibrio vulnificus in estuarine waters of Eastern North Carolina. Appl Environ Microbiol 69(6):3526–3531. doi:10.1128/aem.69.6.3526-3531.2003

    Article  PubMed  CAS  Google Scholar 

  45. O’Neill KR, Jones SH, Grimes DJ (1992) Seasonal incidence of Vibrio vulnificus in the Great Bay estuary of New Hampshire and Maine. Appl Environ Microbiol 58(10):3257–3262

    PubMed  Google Scholar 

  46. Ristori CA, Iaria ST, Gelli DS, Rivera ING (2007) Pathogenic bacteria associated with oysters (Crassostrea brasiliana) and estuarine water along the south coast of Brazil. Int J Environ Heal R 17(4):259–269. doi:10.1080/09603120701372169

    Article  CAS  Google Scholar 

  47. Wright A, Hill R, Johnson J, Roghman M, Colwell R, Morris J Jr (1996) Distribution of Vibrio vulnificus in the Chesapeake Bay. Appl Environ Microbiol 62(2):717–724

    PubMed  CAS  Google Scholar 

  48. Chae M, Cheney D, Su Y (2009) Temperature effects on the depuration of Vibrio parahaemolyticus and Vibrio vulnificus from the American oyster (Crassostrea virginica). J Food Sci 74(2):M62–66

    Article  PubMed  CAS  Google Scholar 

  49. Loosanoff V (1958) Some aspects of behavior of oysters at different temperatures. Biol Bull 114:57–70

    Article  Google Scholar 

  50. Tamplin ML, Capers GM (1992) Persistence of Vibrio vulnificus in tissues of Gulf Coast oysters, Crassostrea virginica, exposed to seawater disinfected with UV light. Appl Environ Microbiol 58(5):1506–1510

    PubMed  CAS  Google Scholar 

  51. Harris-Young L, Tamplin ML, Fisher WS, Mason JW (1993) Effects of physicochemical factors and bacterial colony morphotype on association of Vibrio vulnificus with hemocytes of Crassostrea virginica. Appl Environ Microbiol 59(4):1012–1017

    PubMed  CAS  Google Scholar 

  52. Zimmerman AM, DePaola A, Bowers JC, Krantz JA, Nordstrom JL, Johnson CN, Grimes DJ (2007) Variability of total and pathogenic Vibrio parahaemolyticus densities in northern Gulf of Mexico water and oysters. Appl Environ Microbiol 73(23):7589–7596

    Article  PubMed  CAS  Google Scholar 

  53. Motes M, DePaola A (1996) Offshore suspension relaying to reduce levels of Vibrio vulnificus in oysters (Crassostrea virginica). Appl Environ Microbiol 62(10):3875–3877

    PubMed  CAS  Google Scholar 

  54. Newell RIE, Langdon CJ (1996) Mechanisms and physiology of larval and adult feeding. In: Kennedy VS, Newell RIE, Eble AF (eds) The eastern oyster: Crassostrea virginica. Maryland Sea Grant College, College Park, pp 185–229

    Google Scholar 

  55. Groubert TN, Oliver JD (1994) Interaction of Vibrio vulnificus and the Eastern oyster, Crassostrea virginica. J Food Protect 57(3):224–228

    Google Scholar 

  56. Srivastava M, Tucker MS, Gulig PA, Wright AC (2009) Phase variation, capsular polysaccharide, pilus and flagella contribute to uptake of Vibrio vulnificus by the Eastern oyster (Crassostrea virginica). Environ Microbiol 11(8):1934–1944. doi:10.1111/j.1462-2920.2009.01916.x

    Article  PubMed  Google Scholar 

  57. Rodrick G, Schneider K, Steslow F, Blake N (1987) Uptake, fate and elimination by shellfish in a laboratory depuration system. In: Oceans '87, Sep. 28, 1987–Oct 1, 1987, pp 1752–1756

  58. Kelly MT, Dinuzzo A (1985) Uptake and clearance of Vibrio vulnificus from Gulf coast oysters (Crassostrea virginica). Appl Environ Microbiol 50(6):1548–1549

    PubMed  CAS  Google Scholar 

  59. Lewis M, Rikard S, Arias C (2010) Evaluation of a flow-through depuration system to eliminate the human pathogen Vibrio vulnificus from oysters. J Aquac Res Development 1(103)

  60. Morris JG, Wright AC, Roberts DM, Wood PK, Simpson LM, Oliver JD (1987) Identification of environmental Vibrio vulnificus isolates with a DNA probe for the cytotoxin-hemolysin gene. Appl Environ Microbiol 53(1):193–195

    PubMed  CAS  Google Scholar 

  61. Murphy SK, Oliver JD (1992) Effects of temperature abuse on survival of Vibrio vulnificus in oysters. Appl Environ Microbiol 58(9):2771–2775

    PubMed  CAS  Google Scholar 

  62. Wright AC, Simpson LM, Oliver JD, Morris JG (1990) Phenotypic evaluation of acapsular transposon mutants of Vibrio vulnificus. Infect Immun 58(6):1769–1773

    PubMed  CAS  Google Scholar 

  63. Oliver JD, Roberts DM, White VK, Dry MA, Simpson LM (1986) Bioluminescence in a strain of the human pathogenic bacterium Vibrio vulnificus. Appl Environ Microbiol 52(5):1209–1211

    PubMed  CAS  Google Scholar 

  64. Oliver JD (1993) Formation of viable nut nonculturable cells. In: Kjelleberg S (ed) Starvation in bacteria. Plenum, New York, pp 239–272

    Google Scholar 

  65. Richards GP (1991) Shellfish depuration. In: Ward DR, Hackney C (eds) Microbiology of marine food products. Van Nostrand Reinhold, New York

    Google Scholar 

  66. Capers GM, Tamplin ML, Martin AL, Hopkins LH (1990) Distribution and retention of Vibrio vulnificus in tissues of the Eastern oyster, Crassostrea virginica. Abstr Annu Meating Am Soc Microbiol 305

  67. Greenberg EP, Dubois M, Palhob B (1982) The survival of marine vibrios in Mercenaria mercenaria, the hardshell clam. J Food Safety 4:113–123

    Article  Google Scholar 

  68. Eyles MJ, Davey GR (1984) Microbiology of commercial depuration of the Sydney rock oyster, Crassostrea commercialis. J Food Protect 47:703–706

    Google Scholar 

  69. Sokolova IM, Leamy L, Harrison M, Oliver JD (2005) Intrapopulational variation in Vibrio vulnificus levels in Crassostrea virginica (GMELIN 1971) is associated with the host size but not with disease status or developmental stability. J Shellfish Res 24(2):503–508. doi:10.2983/0730-8000(2005)24[503:ivivvl]2.0.co;2

    Google Scholar 

  70. Paranjpye RN, Johnson AB, Baxter AE, Strom MS (2007) Role of type IV pilins in persistence of Vibrio vulnificus in Crassostrea virginica oysters. Appl Environ Microbiol 73(15):5041–5044. doi:10.1128/aem.00641-07

    Article  PubMed  CAS  Google Scholar 

  71. Grau BL, Henk MC, Pettis GS (2005) High-frequency phase variation of Vibrio vulnificus 1003: isolation and characterization of rugose phenotypic variant. J Bacteriol 187(7):2519–2525

    Article  PubMed  CAS  Google Scholar 

  72. Baker-Austin C, Lemm E, Hartnell R, Lowther J, Onley R, Amaro C, Oliver JD, Lees D (2012) pilF polymorphism-based real-time PCR to distinguish Vibrio vulnificus strains of human health relevance. Food Microbiol 30(1):17–23. doi:10.1016/j.fm.2011.09.002

    Article  PubMed  CAS  Google Scholar 

  73. Roig FJ, Sanjuan E, Llorens A, Amaro C (2010) pilF Polymorphism-based PCR to distinguish Vibrio vulnificus strains potentially dangerous to public health. Appl Environ Microbiol 76(5):1328–1333. doi:10.1128/AEM.01042-09

    Article  PubMed  CAS  Google Scholar 

  74. Ward JE, Shumway SE (2004) Separating the grain from the chaff: particle selection in suspension- and deposit-feeding bivalves. J Exp Mar Biol Ecol 300:83–130

    Article  Google Scholar 

  75. Murphree R, Tamplin M (1995) Uptake and retention of Vibrio cholerae O1 in the Eastern oyster, Crassostrea virginica. Appl Environ Microbiol 61(10):3656–3660

    PubMed  CAS  Google Scholar 

  76. Sun Y, Oliver JD (1995) Hot sauce: no elimination of Vibrio vulnificus in oysters. J Food Protect 58:441–442

    Google Scholar 

  77. Aldrich H, McDowell L, Tamplin M, Frase C, Murphree R, Jackson J (1995) Detection of Vibrio vulnificus and Vibrio cholerae O1 in oyster tissue using immunoelectron microscopy. J Shellfish Res 14(2):493–499

    Google Scholar 

  78. Fisher WS (1986) Structure and functions of oyster hemocytes. In: Brehelin M (ed) Immunity in invertebrates. Springer, Berlin, pp 25–35

    Chapter  Google Scholar 

  79. Genthner FJ, Volety AK, Oliver LM, Fisher WS (1999) Factors influencing in vitro killing of bacteria by hemocytes of the Eastern oyster (Crassostrea virginica). Appl Environ Microbiol 65(7):3015–3020

    PubMed  CAS  Google Scholar 

  80. Tamplin ML, Fisher WS (1989) Occurance and characteristics of agglutination of Vibrio cholerae by serum from the eastern oyster, Crassostrea virginica. Appl Environ Microbiol 55:2882–2887

    PubMed  CAS  Google Scholar 

  81. Tyson CJ, Jenkin CR (1974) Phagocytosis of bacteria in vitro by harmocytes from the crayfish (Parachaeraps bicarinatus). Aust J Exp Biol Med Sci 52:341–348

    Article  PubMed  CAS  Google Scholar 

  82. Harris-Young L, Tamplin M, Mason J, Aldrich H, Jackson J (1995) Viability of Vibrio vulnificus in association with hemocytes of the American oyster (Crassostrea virginica). Appl Environ Microbiol 61(1):52–57

    PubMed  Google Scholar 

  83. Rodrick GE, Ulrich SA (1984) Microscopical studies on the hemocytes of bivalves and their phagocytic interaction with selected bacteria. Helgol Mar Res 37(1):167–176. doi:10.1007/bf01989301

    Google Scholar 

  84. Foley DA, Cheng TC (1975) Degranulation and other changes of molluscan granulocytes associated with phagocytosis. J Invertebr Pathol 25:189–197

    Article  PubMed  CAS  Google Scholar 

  85. McCormick-Ray MG, Howard T (1991) Morphology and mobility of oyster hemocytes: evidence for seasonal variation. J Invertebr Pathol 58:219–230

    Article  PubMed  CAS  Google Scholar 

  86. Oliver JD (1981) Lethal cold stress of Vibrio vulnificus in oysters. Appl Environ Microbiol 41(3):710–717

    PubMed  CAS  Google Scholar 

  87. Pelon W, Luftig RB, Johnston KH (2005) Vibrio vulnificus load reduction in oysters after combined exposure to Vibrio vulnificus-specific bacteriophage and to an oyster extract component. J Food Protect 68(6):1188–1191

    Google Scholar 

  88. Seo JK, Crawford J, Stone K, Noga E (2005) Purification of a novel arthropod defensin from the American oyster, Crassostrea virginica. Biochem Bioph Res Co 338(4):1998–2004

    Article  CAS  Google Scholar 

  89. Seo J-K, Stephenson J, Crawford J, Stone K, Noga E (2010) American oyster, Crassostrea virginica, expresses a potent antibacterial histone H2B protein. Mar Biotechnol 12(5):543–551. doi:10.1007/s10126-009-9240-z

    Article  PubMed  CAS  Google Scholar 

  90. Seo J-K, Stephenson J, Noga EJ (2011) Multiple antibacterial histone H2B proteins are expressed in tissues of American oyster. Comp Biochem Physiol B Biochem Mol Biol 158(3):223–229. doi:10.1016/j.cbpb.2010.11.011

    Article  PubMed  Google Scholar 

  91. Zhang G, Fang X, Guo X, Li L, Luo R, Xu F, Yang P, Zhang L, Wang X, Qi H, Xiong Z, Que H, Xie Y, Holland PWH, Paps J, Zhu Y, Wu F, Chen Y, Wang J, Peng C, Meng J, Yang L, Liu J, Wen B, Zhang N, Huang Z, Zhu Q, Feng Y, Mount A, Hedgecock D, Xu Z, Liu Y, Domazet-Lošo T, Du Y, Sun X, Zhang S, Liu B, Cheng P, Jiang X, Li J, Fan D, Wang W, Fu W, Wang T, Wang B, Zhang J, Peng Z, Li Y, Li N, Wang J, Chen M, He Y, Tan F, Song X, Zheng Q, Huang R, Yang H, Du X, Chen L, Yang M, Gaffney PM, Wang S, Luo L, She Z, Ming Y, Huang W, Zhang S, Huang B, Zhang Y, Qu T, Ni P, Miao G, Wang J, Wang Q, Steinberg CEW, Wang H, Li N, Qian L, Zhang G, Li Y, Yang H, Liu X, Wang J, Yin Y, Wang J (2012) The oyster genome reveals stress adaptation and complexity of shell formation. Nature 490:49–54

    Article  PubMed  CAS  Google Scholar 

  92. Schmitt P, Lorgeril J, Gueguen Y, Destoumieux-Garzón D, Bachère E (2012) Expression, tissue localization and synergy of antimicrobial peptides and proteins in the immune response of the oyster Crassostrea gigas. Dev Comp Immunol 37(3–4):363–370. doi:10.1016/j.dci.2012.01.004

    Article  PubMed  CAS  Google Scholar 

  93. World Health Organization (2005) Risk assessment of Vibrio vulnificus in raw oysters: interpretative summary and technical report. Food and Agriculture Organization, Rome

  94. Ivanina AV, Froelich B, Williams T, Sokolov EP, Oliver JD, Sokolova IM (2011) Interactive effects of cadmium and hypoxia on metabolic responses and bacterial loads of eastern oysters Crassostrea virginica Gmelin. Chemosphere 82(3):377–389. doi:10.1016/j.chemosphere.2010.09.075

    Article  PubMed  CAS  Google Scholar 

  95. Bhattacharya M, Choudhury P, Kumar R (2000) Antibiotic- and metal-resistant strains of Vibrio parahaemolyticus isolated from shrimps. Microb Drug Resist 6:171–172

    Article  PubMed  CAS  Google Scholar 

  96. Fulladosa E, Murat J, Villaescusa I (2005) Effect of cadmium(II), chromium(VI), and arsenic(V) on long-term viability- and growth-inhibition assays using Vibrio fischeri marine bacteria. Arch Environ Contam Toxicol 49:299–306

    Article  PubMed  CAS  Google Scholar 

  97. El-Hendawy HH, Ali DA, Enas H, E-S H, Ghanem SM (2009) Bioaccumulation of heavy metals by Vibrio alginolyticus isolated from wastes of iron and steel factory. Acad J Biol Sci 1:23–28

    Google Scholar 

  98. Kazmi SU, Roberson BS, Stern NJ (1985) Cadmium chloride susceptibility, a characteristic of Campylobacter spp. J Clin Microbiol 21:708–710

    PubMed  CAS  Google Scholar 

  99. Suzuki S, Fukagawa T, Takama K (1992) Occurence of tributyltin-tolerant bacteria in tributyltin- or cadmium-containing seawater. Appl Environ Microbiol 58:3410–3412

    PubMed  CAS  Google Scholar 

  100. Auffret M, Oubella R (1997) Harmocyte aggregation in the oyster Crassostrea gigas: in vitro measurement and experimental modulation by xenobiotics. Comp Biochem Physiol 118A:705–712

    Article  CAS  Google Scholar 

  101. Auffret M, Mujdzic N, Corporeau C, Moraga D (2002) Xenobiotic-induced immunomodulation in the European flat oyster, Ostrea edulis. Mar Environ Res 54:585–589

    Article  PubMed  CAS  Google Scholar 

  102. Brousseau P, Pellerin J, Morin Y, Cyr D, Blakely B, Boermans H, Fournier M (2000) Flow cytometry as a tool to monitor the disturbance of phagocytosis in the clam Mya arenaria haemocytes following in vitro exposure to heavy metals. Toxicology 142:145–156

    Article  PubMed  CAS  Google Scholar 

  103. Roesijadi G, Brubacher LL, Unger ME, Anderson RS (1997) Metallotheionein mRNA induction and generation of reactive oxygen species in molluscan haemocytes exposed to cadmium in vitro. Comp Biochem Physiol 118C:171–176

    CAS  Google Scholar 

  104. Sauve S, Hendawi M, Brousseau P, Fournier M (2002) Phagocytic response of terrestrial and aquatic invertebrates following in vitro exposure to trace elements. Ecotoxicol Environ Saf 52:21–29

    Article  PubMed  CAS  Google Scholar 

  105. Cherkasov AS, Grewal S, Sokolova IM (2007) Combined effects of temperature and cadmium exposure on haemocyte apoptosis and cadmium accumulation in the eastern oyster Crassostrea virginica (Gmelin). J Therm Biol 32:162–170

    Article  CAS  Google Scholar 

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Acknowledgements

We would like to thank Craig Baker-Austin for his examination of the data and suggestion of the review. This material was written with the support of the Cooperative State Research, Education, and Extension Service, US Department of Agriculture, under Award No. 2007-35201-18381. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the US Department of Agriculture.

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  1. The University of North Carolina at Chapel Hill, Institute of Marine Sciences, 3431 Arendell Street, Morehead City, NC, 28557, USA

    Brett Froelich

  2. The University of North Carolina at Charlotte, Charlotte, NC, USA

    James D. Oliver

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  1. Brett Froelich
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Correspondence to Brett Froelich.

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Froelich, B., Oliver, J.D. The Interactions of Vibrio vulnificus and the Oyster Crassostrea virginica . Microb Ecol 65, 807–816 (2013). https://doi.org/10.1007/s00248-012-0162-3

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  • DOI: https://doi.org/10.1007/s00248-012-0162-3

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