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Pyrosequencing Analysis of the Salivary Microbiota of Healthy Chinese Children and Adults

  • Host Microbe Interactions
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Abstract

Describing the biogeography of bacterial communities within the human body is critical for establishing healthy baselines from which to detect differences associated with diseases. Little is known, however, about the baseline of normal salivary microbiota from healthy Chinese children and adults. With parallel barcoded 454 pyrosequencing, the bacterial diversity and richness of saliva were thoroughly investigated from ten healthy Chinese children and adults. The overall taxonomic distribution of our metagenomic data demonstrated that the diversity of salivary microbiota from children was more complex than adults, while the composition and richness of salivary microbiota were similar in children and adults, especially for predominant bacteria. A large number of bacterial phylotypes were shared by healthy children and adults, indicating the existence of a core salivary microbiome. In children and adults, the vast majority of sequences in salivary microbiota belonged to Streptococcus, Prevotella, Neisseria, Haemophilus, Porphyromonas, Gemella, Rothia, Granulicatella, Fusobacterium, Actinomyces, Veillonella, and Aggregatibacter, which constituted the major components of normal salivary microbiota. With the exception of Actinomyces, the other seven non-predominant bacteria including Moraxella, Leptotrichia, Peptostreptococcus, Eubacterium, and members of Neisseriaceae, Flavobacteriaceae, and SR1 showed significant differences between children and adults (p < 0.05). We first established the framework of normal salivary microbiota from healthy Chinese children and adults. Our data represent a critical step for determining the diversity of healthy microbiota in Chinese children and adults, and our data established a platform for additional large-scale studies focusing on the interactions between health and diseases in the future.

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Acknowledgments

We would like to thank Professor Karen E. Nelson for her constructive suggestions of the study and her critical reading of the manuscript. This present work was funded by a grant from the National Basic Research Program of China (973 program) (Grant 2007CB513001) and partly supported by grants from a Qiu-Shi Scholarship from Zhejiang University.

Conflict of Interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China

    Zongxin Ling, Lanjuan Li & Charlie Xiang

  2. Department of Intensive Care Unit, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China

    Xia Liu

  3. Chinese National Human Genome Center at Shanghai, Shanghai, 201203, China

    Yuezhu Wang

  4. J. Craig Venter Institute, Rockville, MD, 20850, USA

    Charlie Xiang

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  1. Zongxin Ling
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  2. Xia Liu
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  4. Lanjuan Li
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  5. Charlie Xiang
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Corresponding authors

Correspondence to Lanjuan Li or Charlie Xiang.

Additional information

Zongxin Ling and Xia Liu contributed equally to this work.

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Table S1

All genera assigned using the RDP-classifier with at least 50 % as bootstrap support (XLS 231 kb)

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Ling, Z., Liu, X., Wang, Y. et al. Pyrosequencing Analysis of the Salivary Microbiota of Healthy Chinese Children and Adults. Microb Ecol 65, 487–495 (2013). https://doi.org/10.1007/s00248-012-0123-x

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  • DOI: https://doi.org/10.1007/s00248-012-0123-x

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