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Increased Temperatures Affect Oxidative Stress Markers and Detoxification Response to Benzo[a]Pyrene Exposure in Mussel Mytilus galloprovincialis

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Abstract

The present research work was designed to study mussel’s (Mytilus galloprovincialis) digestive gland biotransformation and detoxification responses to benzo[a]pyrene (B[a]P) exposure along with heat stress. Mussels were exposed to a sublethal dose of B[a]P [75 nM (19 μg/L/animal)] + temperature gradient (18, 20, 22, 24 and 26 °C) for 7 days. B[a]P hydroxylase (BPH) and glutathione-S-transferase (GST) activities were assessed in digestive gland tissues as phase I and phase II biotransformation parameters. Catalase (CAT) activity and malonedialdehyde (MDA) were measured as potential biomarkers of oxidative stress and lipid peroxidation. The cholinergic system was evaluated using acetylcholinesterase (AChE) activity. DNA damage was assessed using micronuclei (MN) test. BPH and GST activities showed a decreasing trend in B[a]P-exposed animals at 24 and 26 °C. CAT activity showed a bell-shaped response in B[a]P-exposed and in heat-stressed organisms at a maximum temperature of 22 °C. AChE activity was significantly inhibited in response to B[a]P being more pronounced at a temperature of 26 °C. MN in digestive gland cells suggest that B[a]P exposure induced significant DNA alteration with a maximum response in organisms coexposed to B[a]P and a temperature of 26 °C. Biomarker data are further discussed in relation B[a]P accumulation in mussels digestive gland. These data should be carefully considered in view of the biological effects of organic pollutants, particularly in organisms under the challenging effects of extreme temperature fluctuations.

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Acknowledgments

This work was supported by funds from Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Grant No. UR04A6R05), Biochimie et Toxicologie Environnementale, and Theme 6 of the EC Seventh Framework Program through the Marine Ecosystem Evolution in a Changing Environment Collaborative Project (Grant No. MEECE 212085).

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Authors and Affiliations

  1. Laboratory of Biochemistry and Environmental Toxicology, Higher Institute of Agronomy, ISA, Chott-Mariem, 4042, Sousse, Tunisia

    Naouel Kamel, Hajer Attig, Hamadi Boussetta & Mohamed Banni

  2. Department of Environmental and Life Sciences, University of Piemonte Orientale Amedeo Avogadro, Via Bellini 25 G, 15100, Alessandria, Italy

    Alessandro Dagnino

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  1. Naouel Kamel
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  2. Hajer Attig
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  4. Hamadi Boussetta
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  5. Mohamed Banni
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Correspondence to Mohamed Banni.

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Kamel, N., Attig, H., Dagnino, A. et al. Increased Temperatures Affect Oxidative Stress Markers and Detoxification Response to Benzo[a]Pyrene Exposure in Mussel Mytilus galloprovincialis . Arch Environ Contam Toxicol 63, 534–543 (2012). https://doi.org/10.1007/s00244-012-9790-3

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