Journal of Molecular Evolution

, Volume 71, Issue 4, pp 241–249

Evolution of Enzymatic Activities of Testis-Specific Short-Chain Dehydrogenase/Reductase in Drosophila

Authors

  • Jianming Zhang
    • Department of Ecology and EvolutionThe University of Chicago
    • Department of Biological Chemistry and Molecular PharmacologyHarvard Medical School
  • Huyuan Yang
    • Department of Biological Chemistry and Molecular PharmacologyHarvard Medical School
  • Manyuan Long
    • Department of Ecology and EvolutionThe University of Chicago
    • Department of Molecular Pharmacology and Biological ChemistryThe Feinberg School of Medicine, Northwestern University
    • The BioTechnology Institute, University of Minnesota, 240 Gortner Laboratories
Article

DOI: 10.1007/s00239-010-9384-5

Cite this article as:
Zhang, J., Yang, H., Long, M. et al. J Mol Evol (2010) 71: 241. doi:10.1007/s00239-010-9384-5

Abstract

The testis-specific gene Jingwei (jgw) is a newly evolved short-chain dehydrogenase/reductase in Drosophila. Preliminary substrate screening indicated that JGW prefers long-chain primary alcohols as substrates, including several exotic alcohols such as farnesol and geraniol. Using steady-state kinetics analyses and molecular docking, we not only confirmed JGW’s substrate specificity, but also demonstrated that the new enzymatic activities of JGW evolved extensively after exon-shuffling from a preexisting enzyme. Analysis of JGW orthologs in sister species shows that subsequent evolutionary changes following the birth of JGW altered substrate specificities and enzyme stabilities. Our results lend support to a general mechanism for the evolution of a new enzyme, in which catalytic chemistry evolves first followed by diversification of substrate utilization.

Keywords

Enzyme evolutionShort-chain dehydrogenase/reductaseDrosophila alcohol dehydrogenaseExon-shufflingDrosophila

Abbreviations

JGW

Jingwei

SDR

Short-chain dehydrogenase/reductase

ADH

Alcohol dehydrogenase

Supplementary material

239_2010_9384_MOESM1_ESM.doc (330 kb)
Supplementary material: Supplementary material includes data of structural based sequence alignment and Ramachandran Plot of JGW protein: D. teissieri and D. yakuba JGW. (DOC 329 kb)

Copyright information

© Springer Science+Business Media, LLC 2010