Journal of Molecular Evolution

, Volume 68, Issue 6, pp 587–602

The Evolution of Guanylyl Cyclases as Multidomain Proteins: Conserved Features of Kinase-Cyclase Domain Fusions

  • Kabir Hassan Biswas
  • Avinash R. Shenoy
  • Anindya Dutta
  • Sandhya S. Visweswariah
Article

DOI: 10.1007/s00239-009-9242-5

Cite this article as:
Biswas, K.H., Shenoy, A.R., Dutta, A. et al. J Mol Evol (2009) 68: 587. doi:10.1007/s00239-009-9242-5

Abstract

Guanylyl cyclases (GCs) are enzymes that generate cyclic GMP and regulate different physiologic and developmental processes in a number of organisms. GCs possess sequence similarity to class III adenylyl cyclases (ACs) and are present as either membrane-bound receptor GCs or cytosolic soluble GCs. We sought to determine the evolution of GCs using a large-scale bioinformatic analysis and found multiple lineage-specific expansions of GC genes in the genomes of many eukaryotes. Moreover, a few GC-like proteins were identified in prokaryotes, which come fused to a number of different domains, suggesting allosteric regulation of nucleotide cyclase activity. Eukaryotic receptor GCs are associated with a kinase homology domain (KHD), and phylogenetic analysis of these proteins suggest coevolution of the KHD and the associated cyclase domain as well as a conservation of the sequence and the size of the linker region between the KHD and the associated cyclase domain. Finally, we also report the existence of mimiviral proteins that contain putative active kinase domains associated with a cyclase domain, which could suggest early evolution of the fusion of these two important domains involved in signal transduction.

Keywords

Guanylyl cyclaseKinase homology domainMimivirusPhylogenycGMPCoevolution

Supplementary material

239_2009_9242_MOESM1_ESM.doc (294 kb)
Supplementary material 1 (DOC 294 kb)

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Kabir Hassan Biswas
    • 1
  • Avinash R. Shenoy
    • 1
  • Anindya Dutta
    • 1
  • Sandhya S. Visweswariah
    • 1
  1. 1.Department of Molecular Reproduction, Development and GeneticsIndian Institute of ScienceBangaloreIndia