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Nanometer-Scale Permeabilization and Osmotic Swelling Induced by 5-ns Pulsed Electric Fields

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Abstract

High-intensity nanosecond pulsed electric fields (nsPEFs) permeabilize cell membranes. Although progress has been made toward an understanding of the mechanism of nsPEF-induced membrane poration, the dependence of pore size and distribution on pulse duration, strength, number, and repetition rate remains poorly defined experimentally. In this paper, we characterize the size of nsPEF-induced pores in living cell membranes by isosmotically replacing the solutes in pulsing media with polyethylene glycols and sugars before exposing Jurkat T lymphoblasts to 5 ns, 10 MV/m electric pulses. Pore size was evaluated by analyzing cell volume changes resulting from the permeation of osmolytes through the plasma membrane. We find that pores created by 5 ns pulses have a diameter between 0.7 and 0.9 nm at pulse counts up to 100 with a repetition rate of 1 kHz. For larger number of pulses, either the pore diameter or the number of pores created, or both, increase with increasing pulse counts. But the prevention of cell swelling by PEG 1000 even after 2000 pulses suggests that 5 ns, 10 MV/m pulses cannot produce pores with a diameter larger than 1.9 nm.

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Acknowledgments

EBS and PTV received support from the Frank Reidy Research Center for Bioelectrics at Old Dominion University and from the Air Force Office of Scientific Research (FA9550-15-1-0517, FA9550-14-1-0123).

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Authors and Affiliations

  1. Frank Reidy Research Center for Bioelectrics, Old Dominion University, 4211 Monarch Way. STE 300, Norfolk, VA, USA

    Esin B. Sözer & P. Thomas Vernier

  2. Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA

    Yu-Hsuan Wu

  3. CNR - Institute for Electromagnetic Sensing of the Environment (IREA), Via Diocleziano 328, 80124, Naples, Italy

    Stefania Romeo

Authors
  1. Esin B. Sözer
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  2. Yu-Hsuan Wu
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  3. Stefania Romeo
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  4. P. Thomas Vernier
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Correspondence to Esin B. Sözer.

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Sözer, E.B., Wu, YH., Romeo, S. et al. Nanometer-Scale Permeabilization and Osmotic Swelling Induced by 5-ns Pulsed Electric Fields. J Membrane Biol 250, 21–30 (2017). https://doi.org/10.1007/s00232-016-9918-x

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  • DOI: https://doi.org/10.1007/s00232-016-9918-x

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