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The Influence of Fatty Acid Methyl Esters (FAMEs) in the Biochemistry and the Na+/K+-ATPase Activity of Culex quinquefasciatus Larvae

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Abstract

Culex quinquefasciatus is the main vector of lymphatic filariasis and combating this insect is of great importance to public health. There are reports of insects that are resistant to the products currently used to control this vector, and therefore, the search for new products has increased. In the present study, we have evaluated the effects of fatty acid methyl esters (FAMEs) that showed larvicidal activity against C. quinquefasciatus, on glucose, total protein, and triacylglycerol contents and Na+/K+-ATPase activity in mosquito larvae. The exposure of the fourth instar larvae to the compounds caused a decrease in the total protein content and an increase in the activity of the Na+/K+-ATPase. Furthermore, the direct effect of FAMEs on cell membrane was assessed on purified pig kidney Na+/K+-ATPase membranes, erythrocyte ghost membranes, and larvae membrane preparation. No modifications on total phospholipids and cholesterol content were found after FAMEs 20 min treatment on larvae membrane preparation, but only 360 µg/mL FAME 2 was able to decrease total phospholipid of erythrocyte ghost membrane. Moreover, only 60 and 360 µg/mL FAME 3 caused an activation of purified Na+/K+-ATPase, that was an opposite effect of FAMEs treatment in larvae membrane preparation, and caused an inhibition of the pump activity. These data together suggest that maybe FAMEs can modulate the Na+/K+-ATPase on intact larvae for such mechanisms and not for a direct effect, one time that the direct effect of FAMEs in membrane preparation decreased the activity of Na+/K+-ATPase. The biochemical changes caused by the compounds were significant and may negatively influence the development and survival of C. quinquefasciatus larvae.

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Acknowledgments

This work was supported by FAPEMIG (Fundação de Amparo a Pesquisa do Estado de Minas Gerais) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico). We thank Jessica Venugopal for critically reading the manuscript.

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Authors and Affiliations

  1. Laboratório de Bioquímica Celular, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindu, Av Sebastião Gonçalves Coelho 400, Bairro Chanadour, Divinópolis, MG, CEP 35501-296, Brazil

    Lilian N. D. Silva, Jéssica M. M. Valadares, Vanessa F. Cortes, Herica L. Santos & Leandro A. Barbosa

  2. Laboratório de Insetos Vetores de Doenças, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindu, Av Sebastião Gonçalves Coelho 400, Divinópolis, MG, 35501-296, Brazil

    José A. Ribeiro-Neto & Stênio N. Alves

  3. Laboratório de Fitoquímica, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindu, Av Sebastião Gonçalves Coelho 400, Divinópolis, MG, 35501-296, Brazil

    Luciana A. R. S. Lima

  4. Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas, Campus A C Simões, Av Lourival de Melo Mota, Tabuleiro do Martins, Maceió, AL, 57072-900, Brazil

    Mariana M. Costa & Luciano A. M. Grillo

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  1. Lilian N. D. Silva
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  2. José A. Ribeiro-Neto
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  3. Jéssica M. M. Valadares
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  6. Luciano A. M. Grillo
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Correspondence to Leandro A. Barbosa.

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Silva, L.N.D., Ribeiro-Neto, J.A., Valadares, J.M.M. et al. The Influence of Fatty Acid Methyl Esters (FAMEs) in the Biochemistry and the Na+/K+-ATPase Activity of Culex quinquefasciatus Larvae. J Membrane Biol 249, 459–467 (2016). https://doi.org/10.1007/s00232-016-9886-1

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