The Journal of Membrane Biology

, Volume 247, Issue 9, pp 815–826

Functionalized Amphipols: A Versatile Toolbox Suitable for Applications of Membrane Proteins in Synthetic Biology

Authors

  • Eduardo Antonio Della Pia
    • Bio-Nanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-Science CenterUniversity of Copenhagen
  • Randi Westh Hansen
    • Bio-Nanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-Science CenterUniversity of Copenhagen
  • Manuela Zoonens
    • Institut de Biologie Physico-ChimiqueUMR 7099, CNRS/Université Paris-7
    • Bio-Nanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-Science CenterUniversity of Copenhagen
Article

DOI: 10.1007/s00232-014-9663-y

Cite this article as:
Della Pia, E.A., Hansen, R.W., Zoonens, M. et al. J Membrane Biol (2014) 247: 815. doi:10.1007/s00232-014-9663-y

Abstract

Amphipols are amphipathic polymers that stabilize membrane proteins isolated from their native membrane. They have been functionalized with various chemical groups in the past years for protein labeling and protein immobilization. This large toolbox of functionalized amphipols combined with their interesting physico-chemical properties give opportunities to selectively add multiple functionalities to membrane proteins and to tune them according to the needs. This unique combination of properties makes them one of the most versatile strategies available today for exploiting membrane proteins onto surfaces for various applications in synthetic biology. This review summarizes the properties of functionalized amphipols suitable for synthetic biology approaches.

Keywords

BiosensorsSelf-assemblyProtein immobilizationProtein labelingMultiple functionalization

Abbreviations

A8-35

Poly(sodium acrylate)-based amphipol comprising 35 % of free carboxylate, 25 % of octyl chains and 40 % of isopropyl groups

APol

Amphipol

BAPol

Biotinylated A8-35

BG

Benzyl guanine

BR

Bacteriorhodopsin

CMC

Critical micelle concentration

DAPol

Deuterated A8-35

E. coli

Escherichia coli

FAPol

Fluorescently labeled A8-35

GFP

Green fluorescent protein

GPCR

G protein-coupled receptor

HAPol

Hydrogenated A8-35

HistAPol

Histidine-tagged A8-35

ImidAPol

Imidazol-tagged A8-35

MPs

Membrane proteins

NAPol

Non-ionic glycosylated APol

NBD

7-Nitro-1,2,3-benzoxadiazole

ND

Nanodisc

NP

Nanoparticle

NTA

Nitriloacetic acid

OligAPol

Oligodeoxynucleotide tagged A8-35

PerDAPol

Perdeuterated A8-35

POR

Cytochrome P450 oxidoreductase

SAPol

Sulfonated APol

SPR

Surface plasmon resonance

tOmpA

Transmembrane domain of the Escherichia coli outer membrane protein A

Copyright information

© Springer Science+Business Media New York 2014