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Actin Microfilament Involved in Regulation of Pacemaking Activity in Cultured Interstitial Cells of Cajal from Murine Intestine

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Abstract

The present study investigated the effect of actin microfilament structure on pacemaker currents and calcium oscillation in cultured murine intestinal interstitial cells of Cajal (ICCs) by whole-cell patch-clamp technique and calcium imaging technique. Cytochalasin B, a disruptor of actin microfilaments, decreased the amplitude and frequency of pacemaker currents from 491.32 ± 160.33 pA and 11.73 ± 0.79 cycles/min to 233.12 ± 92.00 pA and 10.29 ± 0.76 cycles/min. Cytochalasin B also decreased the amplitude and frequency of calcium oscillation from 0.32 ± 0.08 (ΔF/F0) and 2.75 ± 0.17 cycles/min to 0.02 ± 0.01 (ΔF/F0) and 1.20 ± 0.08 cycles/min. Phalloidin, a stabilizer of actin microfilaments, increased the amplitude and frequency of pacemaker currents from 751.79 ± 282.82 pA and 13.93 ± 1.00 cycles/min to 1234.34 ± 607.83 pA and 14.68 ± 1.00 cycles/min. Phalloidin also increased the amplitude and frequency of calcium oscillation from 0.26 ± 0.01 (ΔF/F0) and 2.27 ± 0.18 cycles/min to 0.43 ± 0.03 (ΔF/F0) and 2.87 ± 0.07 cycles/min. 2-Aminoethoxydiphenyl borane (2-APB), an IP3 receptor blocker, suppressed both pacemaker currents and calcium oscillations. 2-APB also blocked the phalloidin-induced increase in pacemaker currents and calcium oscillation. Ryanodine, an inhibitor of calcium-induced calcium release, did not affect pacemaker current but suppressed calcium oscillations. Ryanodine had no effect on altering phalloidin-induced increases in pacemaker current and calcium oscillation. These results suggest that actin microfilaments regulate pacemaker activity via the IP3-induced calcium release signaling pathway.

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Acknowledgment

This study was funded by grant 10672103 provided by the National Natural Science Foundation of China.

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Authors and Affiliations

  1. Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai, 200240, China

    Zuo Yu Wang, Yan Fei Han, Xu Huang, Hong Li Lu, Xin Guo & Wen Xie Xu

  2. Department of Physiology, Chungbuk National University College of Medicine, 12 Gaeshin-dong, Hungduk-gu, Cheongju, Chungbuk, 361-763, Korea

    Yong Chul Kim

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  1. Zuo Yu Wang
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  2. Yan Fei Han
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  3. Xu Huang
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  4. Hong Li Lu
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  5. Xin Guo
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  7. Wen Xie Xu
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Correspondence to Wen Xie Xu.

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Wang, Z.Y., Han, Y.F., Huang, X. et al. Actin Microfilament Involved in Regulation of Pacemaking Activity in Cultured Interstitial Cells of Cajal from Murine Intestine. J Membrane Biol 234, 217–225 (2010). https://doi.org/10.1007/s00232-010-9248-3

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  • DOI: https://doi.org/10.1007/s00232-010-9248-3

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