Journal of Membrane Biology

, Volume 234, Issue 3, pp 217–225

Actin Microfilament Involved in Regulation of Pacemaking Activity in Cultured Interstitial Cells of Cajal from Murine Intestine

  • Zuo Yu Wang
  • Yan Fei Han
  • Xu Huang
  • Hong Li Lu
  • Xin Guo
  • Yong Chul Kim
  • Wen Xie Xu
Article

DOI: 10.1007/s00232-010-9248-3

Cite this article as:
Wang, Z.Y., Han, Y.F., Huang, X. et al. J Membrane Biol (2010) 234: 217. doi:10.1007/s00232-010-9248-3

Abstract

The present study investigated the effect of actin microfilament structure on pacemaker currents and calcium oscillation in cultured murine intestinal interstitial cells of Cajal (ICCs) by whole-cell patch-clamp technique and calcium imaging technique. Cytochalasin B, a disruptor of actin microfilaments, decreased the amplitude and frequency of pacemaker currents from 491.32 ± 160.33 pA and 11.73 ± 0.79 cycles/min to 233.12 ± 92.00 pA and 10.29 ± 0.76 cycles/min. Cytochalasin B also decreased the amplitude and frequency of calcium oscillation from 0.32 ± 0.08 (ΔF/F0) and 2.75 ± 0.17 cycles/min to 0.02 ± 0.01 (ΔF/F0) and 1.20 ± 0.08 cycles/min. Phalloidin, a stabilizer of actin microfilaments, increased the amplitude and frequency of pacemaker currents from 751.79 ± 282.82 pA and 13.93 ± 1.00 cycles/min to 1234.34 ± 607.83 pA and 14.68 ± 1.00 cycles/min. Phalloidin also increased the amplitude and frequency of calcium oscillation from 0.26 ± 0.01 (ΔF/F0) and 2.27 ± 0.18 cycles/min to 0.43 ± 0.03 (ΔF/F0) and 2.87 ± 0.07 cycles/min. 2-Aminoethoxydiphenyl borane (2-APB), an IP3 receptor blocker, suppressed both pacemaker currents and calcium oscillations. 2-APB also blocked the phalloidin-induced increase in pacemaker currents and calcium oscillation. Ryanodine, an inhibitor of calcium-induced calcium release, did not affect pacemaker current but suppressed calcium oscillations. Ryanodine had no effect on altering phalloidin-induced increases in pacemaker current and calcium oscillation. These results suggest that actin microfilaments regulate pacemaker activity via the IP3-induced calcium release signaling pathway.

Keywords

Patch clamp techniqueCalcium imaging techniqueInterstitial cells of CajalActin microfilamentPacemaker currentCalcium oscillation

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Zuo Yu Wang
    • 1
  • Yan Fei Han
    • 1
  • Xu Huang
    • 1
  • Hong Li Lu
    • 1
  • Xin Guo
    • 1
  • Yong Chul Kim
    • 2
  • Wen Xie Xu
    • 1
  1. 1.Department of PhysiologyShanghai Jiaotong University School of MedicineShanghaiChina
  2. 2.Department of PhysiologyChungbuk National University College of MedicineChungbukKorea