Marine Biology

, Volume 151, Issue 5, pp 1799–1812

Spatiotemporal analysis of population genetic structure in Geomonhystera disjuncta (Nematoda, Monhysteridae) reveals high levels of molecular diversity

  • S. Derycke
  • T. Backeljau
  • C. Vlaeminck
  • A. Vierstraete
  • J. Vanfleteren
  • M. Vincx
  • T. Moens
Research Article

DOI: 10.1007/s00227-007-0609-0

Cite this article as:
Derycke, S., Backeljau, T., Vlaeminck, C. et al. Mar Biol (2007) 151: 1799. doi:10.1007/s00227-007-0609-0

Abstract

Species identification in the phylum Nematoda is complicated due to the paucity of easily obtainable diagnostic morphological features. Furthermore, the cosmopolitan distribution of several species despite low dispersal abilities makes cryptic diversity potentially substantial within this phylum. We conducted a population genetic survey in the marine nematode Geomonhystera disjuncta in Belgium and The Netherlands in two seasons. The mitochondrial cytochrome oxidase c subunit 1 (COI) gene was screened with the single-strand conformation polymorphism method in 759 individuals. The 43 haplotypes were grouped into five lineages, with low divergences within (<3%) and high divergences between lineages (>14%). Analysis of the nuclear ITS region yielded concordant tree topologies, indicating the presence of five cryptic taxa within G. disjuncta. Analysis of Molecular Variance (AMOVA) illustrated a significant structuring in all lineages and temporal fluctuations in haplotype frequencies within and between locations. Metapopulation dynamics and/or priority effects best explained this structuring. Finally, our data indicate that the COI gene may be useful for DNA barcoding purposes.

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • S. Derycke
    • 1
    • 5
  • T. Backeljau
    • 2
    • 3
  • C. Vlaeminck
    • 1
  • A. Vierstraete
    • 4
    • 5
  • J. Vanfleteren
    • 4
    • 5
  • M. Vincx
    • 1
  • T. Moens
    • 1
  1. 1.Department of Biology, Marine Biology SectionGhent UniversityGhentBelgium
  2. 2.Royal Belgian Institute of Natural SciencesBrusselsBelgium
  3. 3.Evolutionary Biology Group, Department of BiologyUniversity of AntwerpAntwerpBelgium
  4. 4.Department of BiologyGhent UniversityGhentBelgium
  5. 5.CeMoFe, Ghent UniversityGhentBelgium