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DNA microsatellite variability and genetic differentiation among natural populations of the Cuban white shrimp Litopenaeus schmitti

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Abstract

Genetic variability within the Cuban population of the white shrimp Litopenaeus schmitti (Burkenroad, 1936) was assessed using five microsatellite loci and samples collected from four sites (Batabanó, Cienfuegos, Tunas de Zaza, and Manzanillo) from November 1999 to March 2000. All loci were polymorphic, and a total of 80 alleles were found, 13 of them private alleles occurring only in a single population and always in low frequencies. The Cienfuegos sample had the highest observed heterozygosity (H o=0.653), and the Tunas de Zaza sample had the lowest values (H o=0.605), but there were no significant differences among samples in heterozygosity or in the mean numbers of alleles per locus (ranging from 11.0 to 11.6). Significant differentiation among populations was detected (F ST=0.012, P<0.001). Low but significant F ST values were revealed in pairwise comparisons between populations. Assignation tests correctly assigned high percentages of individuals to their original populations (74.5%) using a Bayesian approach. The significant differentiation among populations could be due to the restriction of gene flow among populations of L. schmitti and is concordant with previous allozyme studies on Cuban populations.

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References

  • Alfonso I, Frias MP, Baisre J, Campos A (1991) Distribución y abundancia de larvas de la langosta Panulirus argus en aguas alrededor de Cuba. Rev Investig Mar 12:5–19

    Google Scholar 

  • Ball AO, Leonard S, Chapman RW (1998) Characterization of (GT) n from native white shrimp (Penaeus setiferus). Mol Ecol 7:1251–1253

    CAS  PubMed  Google Scholar 

  • Benzie JAH (2000) Population genetic structure in penaeid prawns. Aquac Res 31:95–119

    Article  Google Scholar 

  • Benzie JAH, Frusher S, Ballment E (1992) Geographical variation in allozyme frequencies of Penaeus monodon (Crustacea: Decapoda) populations in Australia. Aust J Mar Freshw Res 43:715–725

    Google Scholar 

  • Bierne N, Beuzart I, Vonau V, Bonhomme F, Bédier E, AQUACOP (2000) Microsatellite-associated heterosis in hatchery-propagated stocks of the shrimp Penaeus stylirostris. Aquaculture 184:203–219

    Article  Google Scholar 

  • Brooker AL, Benzie JAH, Blair D, Versini JJ (1999) Population structure of the giant tiger prawn Penaeus monodom in Australian waters, determined using microsatellite markers. Mar Biol 136:149–157

    Article  Google Scholar 

  • Burkenroad MD (1936) A new species of Penaeus from the American Atlantic. An Acad Bras Cienc Rio de Janeiro 8:315–318

    Google Scholar 

  • Chakraborty R, Haag M, Ryman N, Stahl G (1982) Hierarchical gene diversity analysis and its application to brown trout population data. Hereditas 97:17–21

    Google Scholar 

  • Cornuet JM, Piry S, Luikart G, Estoup A, Solignac M (1999) Comparison of methods employing multilocus genotypes to select or exclude populations as origins of individuals. Genetics 153:1989–2000

    CAS  PubMed  Google Scholar 

  • Dall W, Hill BJ, Rothlisberg PC, Staples DJ (1990) The biology of Penaeidea. Adv Mar Biol 27:1–489

    Google Scholar 

  • Espinosa G, Jager M, Machado E, Borrell Y, Corona N, Robainas A, Deutsh J (2001) Microsatellites from the white shrimp Litopenaeus schmitti (Crustacea, Decapoda). Biotecnol Apl 18:19–22

    Google Scholar 

  • Espinosa G, Díaz R, Matos J, Becquer U, Romo J, Borrell Y (2003) Variación aloenzimática en poblaciones cubanas del camarón blanco L. schmitti. Rev Investig Mar 24:11–19

    Google Scholar 

  • Felsenstein J (1993) PHYLIP (phylogeny inference package). Dept. Genetics, University of Washington, Seattle

  • Forbes AT, Demetriades NT, Benzie JAH, Ballment E (1999) Allozyme frequencies indicate little geographic variation amongst stocks of the giant tiger prawn, Penaeus monodon, in the south-east Indian Ocean. S Afr J Mar Biol 21:271–277

    Google Scholar 

  • García C, Chirino A, Rodríguez J (1991) Corrientes geostróficas en la ZEE al sur de Cuba. Rev Investig Mar 12:29–38

    Google Scholar 

  • García D, Dhar AK, Alcívar-Warren A (1996) Molecular analysis of a RAPD marker (B20) reveals two microsatellites and differential mRNA expression in Penaeus vannamei. Mol Mar Biol Biotechnol 5:71–83

    PubMed  Google Scholar 

  • García-Machado E, Robainas A, Espinosa G, Oliva M, Páez J, Verdecia N, Monnerot M (2001) Allozyme and mitochondrial DNA variation in Cuban populations of the shrimp Farfantepenaeus notialis (Crustacea, Decapoda). Mar Biol 138:701–707

    Article  Google Scholar 

  • Goudet J (1995) FSTAT (vers. 1.2): a computer program to calculate F-statistics. J Hered 86:485–486

    Google Scholar 

  • Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from http://www.unil.ch/izea/softwares/fstat.html

  • Guitart B, González E, Fraga I, Reyes R (1985) Áreas y épocas de desove de los camarones Penaeus notialis y Penaeus schmitti en la plataforma cubana. Rev Investig Pesq 10:58–76

    Google Scholar 

  • Hansen M, Kenchington E, Nielsen E (2001) Assigning individual fish to populations using microsatellite DNA markers. Fish Fish Ser 2:93–112

    Article  Google Scholar 

  • Ibarra-Martín C, Vega R, Espinosa R, Dominguez R, Carasa J (1978) Atlas de Cuba. Instituto de Geodesia y Cartografía, Havana, Cuba

  • Klibunga S, Penman DJ, McAndrew BJ, Tassanakajon A, Jarayabhand P (1998) Genetic variation, population differentiation, and gene flow of the giant tiger shrimp Penaeus monodon inferred from mtDNA-RFLP data. In: Flegel TW (ed) Advances in shrimp biotechnology. National Center for Genetic Engineering and Biotechnology, Bangkok, pp 51–59

  • Lester LJ (1979) Population genetics of penaeid shrimp from the Gulf of Mexico. J Hered 70:175–180

    Google Scholar 

  • Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220

    CAS  PubMed  Google Scholar 

  • Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655

    CAS  PubMed  Google Scholar 

  • Moore SS, Whan V, Davis G, Byrne K, Hetzel D, Preston N (1999) The development and application of genetic markers for the Kuruma prawn Penaeus japonicus. Aquaculture 173:19–32

    Article  CAS  Google Scholar 

  • Mulley JC, Latter BDH (1980) Genetic variation and evolutionary relationship within a group of thirteen species of penaeid prawns. Evolution 34:904–916

    CAS  Google Scholar 

  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590

    Google Scholar 

  • Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York

  • Page RDM (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    CAS  PubMed  Google Scholar 

  • Pemberton JM, Slate J, Bancroft DR, Barrett JA (1995) Nonamplifying alleles at microsatellite loci: a caution for parentage and population studies. Mol Ecol 4:249–252

    CAS  PubMed  Google Scholar 

  • Perez-Farfante I, Kensley BF (1997) Penaeoid and sergestoid shrimps and prawns of the world: keys and diagnoses for the families and genera. Mem Mus Natl Hist Nat 175:1–233

    Google Scholar 

  • Pongsomboon S, Whan V, Moore SS, Tassanakajon A (2000) Characterization of tri- and tetranucleotide microsatellites in the black tiger prawn Penaeus monodon. Sci Asia 26:1–8

    CAS  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Rice WR (1989) Analysing tables of statistical tests. Evolution 43:223–225

    Google Scholar 

  • Summers K, Amos B (1997) Behavioral ecological and molecular genetic analyses of reproductive strategies in the Amazonian dart-poison frog, Dendrobates ventrimaculatus. Behav Ecol 8:260–267

    Google Scholar 

  • Swofford DL, Selander RB (1981) Biosys-1: a FORTRAN program for the comprehensive analysis for electrophoretic data in population genetics and systematics. J Hered 72:281–283

    Google Scholar 

  • Tassanakajon A, Tiptawonnukul A, Supangul P, Rimphanitchayakit V, Cook D, Jarayabhand P, Klinbunga S, Boonsaeng V (1998a) Isolation and characterization of microsatellite markers in the black tiger prawn Penaeus monodon. Mol Mar Biol Biotechnol 7:55–61

    CAS  Google Scholar 

  • Tassanakajon A, Supangul P, Klinbunga S, Jarayabhand P, Boosaeng V (1998b) Microsatellite variation in wild populations of the black tiger prawn Penaeus monodon in Thailand. In: Larkin P (ed) Agricultural biotechnology: laboratory, field and market. Proceedings of the 4th Asia–Pacific conference on agricultural biotechnology. CPN Publications, Canberra, pp 255–257

  • Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10:506–510

    CAS  PubMed  Google Scholar 

  • Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370

    Google Scholar 

  • Wolfus GM, García GK, Alcivar-Warren A (1997) Application of the microsatellites technique for analyzing genetic diversity in shrimp breeding programs. Aquaculture 152:35–47

    Article  CAS  Google Scholar 

  • Xu Z, Dhar AK, Wyrzykowski J, Alcivar-Warren A (1999) Identification of abundant and informative microsatellites from shrimp (Penaeus monodon). Anim Genet 30:150–156

    Article  CAS  PubMed  Google Scholar 

  • Xu Z, Primavera JH, De la Pena L, Petit P, Belak J, Alcivar-Warren A (2001) Genetic diversity of wild and cultured black tiger shrimp (Penaeus monodon) in the Philippines using microsatellites. Aquaculture 199:13–40

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank two anonymous reviewers for their useful suggestions. Thanks also to P. Hedges (Pentolenviro, UK) who helped us with language corrections. Y.J. Borrell had an AECI fellowship during the development of this work.

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Correspondence to Y. Borrell.

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Communicated by J.P. Grassle, New Brunswick

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Borrell, Y., Espinosa, G., Romo, J. et al. DNA microsatellite variability and genetic differentiation among natural populations of the Cuban white shrimp Litopenaeus schmitti . Marine Biology 144, 327–333 (2004). https://doi.org/10.1007/s00227-003-1198-1

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