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Potential of Osteoblastic Cells Derived from Bone Marrow and Adipose Tissue Associated with a Polymer/Ceramic Composite to Repair Bone Tissue

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Abstract

One of the tissue engineering strategies to promote bone regeneration is the association of cells and biomaterials. In this context, the aim of this study was to evaluate if cell source, either from bone marrow or adipose tissue, affects bone repair induced by osteoblastic cells associated with a membrane of poly(vinylidene-trifluoroethylene)/barium titanate (PVDF-TrFE/BT). Mesenchymal stem cells (MSC) were isolated from rat bone marrow and adipose tissue and characterized by detection of several surface markers. Also, both cell populations were cultured under osteogenic conditions and it was observed that MSC from bone marrow were more osteogenic than MSC from adipose tissue. The bone repair was evaluated in rat calvarial defects implanted with PVDF-TrFE/BT membrane and locally injected with (1) osteoblastic cells differentiated from MSC from bone marrow, (2) osteoblastic cells differentiated from MSC from adipose tissue or (3) phosphate-buffered saline. Luciferase-expressing osteoblastic cells derived from bone marrow and adipose tissue were detected in bone defects after cell injection during 25 days without difference in luciferin signal between cells from both sources. Corroborating the in vitro findings, osteoblastic cells from bone marrow combined with the PVDF-TrFE/BT membrane increased the bone formation, whereas osteoblastic cells from adipose tissue did not enhance the bone repair induced by the membrane itself. Based on these findings, it is possible to conclude that, by combining a membrane with cells in this rat model, cell source matters and that bone marrow could be a more suitable source of cells for therapies to engineer bone.

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Acknowledgements

Fabiana Rosseto de Moraes, Fabíola Singaretti de Oliveira, Milla Sprone Tavares, Roger Rodrigo Fernandes and Sebastião Carlos Bianco are acknowledged for their technical assistance during the experiments.

Funding

This research was supported by the National Council of Technological and Scientific Development (Grant # 456871/2013-6, CNPq - Brazil).

Author information

Authors and Affiliations

  1. Cell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo, Av do Café s/n, Ribeirão Preto, SP, 14040-904, Brazil

    Gileade P. Freitas, Helena B. Lopes, Adriana L. G. Almeida, Rodrigo P. F. Abuna, Marcio M. Beloti & Adalberto L. Rosa

  2. Institute of Physics and Chemistry, Federal University of Itajubá, Itajubá, MG, Brazil

    Rossano Gimenes

  3. National Institute of Science and Technology in Stem Cell and Cell Therapy, Ribeirão Preto, SP, Brazil

    Lucas E. B. Souza & Dimas T. Covas

  4. Department of Clinical Medicine, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil

    Lucas E. B. Souza & Dimas T. Covas

Authors
  1. Gileade P. Freitas
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  2. Helena B. Lopes
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  3. Adriana L. G. Almeida
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  4. Rodrigo P. F. Abuna
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  5. Rossano Gimenes
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  6. Lucas E. B. Souza
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  7. Dimas T. Covas
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  8. Marcio M. Beloti
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  9. Adalberto L. Rosa
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Corresponding author

Correspondence to Adalberto L. Rosa.

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Conflict of interest

Gileade P. Freitas, Helena B. Lopes, Adriana L. G. Almeida, Rodrigo P. F. Abuna, Rossano Gimenes, Lucas E. B. Souza, Dimas T. Covas, Marcio M. Beloti and Adalberto L. Rosa declare that they have no conflict of interest associated with this study and there has been no financial support that could have influenced our outcomes.

Human and Animal Rights and Informed Consent

All applicable international, national and/or institutional guidelines for the care and use of animals were followed. The Committee of Ethics in Animal Research of the School of Dentistry of Ribeirão Preto, University of São Paulo approved all animal procedures performed during the experiments.

Electronic Supplementary Material

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223_2017_282_MOESM1_ESM.tif

Supplementary material 1 (TIFF 3238 kb) FIG. S1. Three-dimensional reconstructed micro-CT image (A) and light microscopy (B) of an untreated rat calvarial bone defect at 4 weeks. No significant bone formation was noticed in the defect (A), which was filled with connective tissue (B). Alizarin red and Stevenel’s blue stain. Scale bar: A = 1.5 mm and B = 200 μm

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Freitas, G.P., Lopes, H.B., Almeida, A.L.G. et al. Potential of Osteoblastic Cells Derived from Bone Marrow and Adipose Tissue Associated with a Polymer/Ceramic Composite to Repair Bone Tissue. Calcif Tissue Int 101, 312–320 (2017). https://doi.org/10.1007/s00223-017-0282-3

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  • DOI: https://doi.org/10.1007/s00223-017-0282-3

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