Abstract
The skeleton serves as the principal site for hematopoiesis in adult terrestrial vertebrates. The function of the hematopoietic system is to maintain homeostatic levels of all circulating blood cells, including myeloid cells, lymphoid cells, red blood cells, and platelets. This action requires the daily production of more than 500 billion blood cells. The vast majority of these cells are synthesized in the bone marrow, where they arise from a limited number of hematopoietic stem cells (HSCs) that are multipotent and capable of extensive self-renewal. These attributes of HSCs are best demonstrated by marrow transplantation, where even a single HSC can repopulate the entire hematopoietic system. HSCs are therefore adult stem cells capable of multilineage repopulation, poised between cell fate choices which include quiescence, self-renewal, differentiation, and apoptosis. While HSC fate choices are in part determined by multiple stochastic fluctuations of cell autonomous processes, according to the niche hypothesis, signals from the microenvironment are also likely to determine stem cell fate. While it had long been postulated that signals within the bone marrow could provide regulation of hematopoietic cells, it is only in the past decade that advances in flow cytometry and genetic models have allowed for a deeper understanding of the microenvironmental regulation of HSCs. In this review, we will highlight the cellular regulatory components of the HSC niche.
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References
Hartenstein V (2006) Blood cells and blood cell development in the animal kingdom. Annu Rev Cell Dev Biol 22:677–712
Bianco P, Robey PG et al (2010) “Mesenchymal” stem cells in human bone marrow (skeletal stem cells): a critical discussion of their nature, identity, and significance in incurable skeletal disease. Hum Gene Ther 21(9):1057–1066
Chang MK, Raggatt LJ et al (2008) Osteal tissue macrophages are intercalated throughout human and mouse bone lining tissues and regulate osteoblast function in vitro and in vivo. J Immunol 181(2):1232–1244
Fazeli PK, Horowitz MC et al (2013) Marrow fat and bone—new perspectives. J Clin Endocrinol Metab 98(3):935–945
Wilson A, Murphy MJ et al (2004) c-Myc controls the balance between hematopoietic stem cell self-renewal and differentiation. Genes Dev 18(22):2747–2763
Xie Y, Yin T et al (2009) Detection of functional haematopoietic stem cell niche using real-time imaging. Nature 457(7225):97–101
Zhang J, Niu C et al (2003) Identification of the haematopoietic stem cell niche and control of the niche size. Nature 425(6960):836–841
Kiel MJ, Yilmaz OH et al (2005) SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell 121(7):1109–1121
Sugiyama T, Kohara H et al (2006) Maintenance of the hematopoietic stem cell pool by CXCL12-CXCR4 chemokine signaling in bone marrow stromal cell niches. Immunity 25(6):977–988
Mendez-Ferrer S, Michurina TV et al (2010) Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. Nature 466(7308):829–834
Nombela-Arrieta C, Pivarnik G et al (2013) Quantitative imaging of haematopoietic stem and progenitor cell localization and hypoxic status in the bone marrow microenvironment. Nat Cell Biol 15(5):533–543
Cantor AB, Orkin SH (2001) Hematopoietic development: a balancing act. Curr Opin Genet Dev 11(5):513–519
Enver T, Pera M et al (2009) Stem cell states, fates, and the rules of attraction. Cell Stem Cell 4(5):387–397
Graf T, Enver T (2009) Forcing cells to change lineages. Nature 462(7273):587–594
Schofield R (1978) The relationship between the spleen colony-forming cell and the haemopoietic stem cell. Blood Cells 4(1–2):7–25
Gong JK (1978) Endosteal marrow: a rich source of hematopoietic stem cells. Science 199(4336):1443–1445
Lord BI, Testa NG et al (1975) The relative spatial distributions of CFUs and CFUc in the normal mouse femur. Blood 46(1):65–72
Lo Celso C, Fleming HE et al (2009) Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche. Nature 457(7225):92–96
Nilsson SK, Dooner MS et al (1997) Potential and distribution of transplanted hematopoietic stem cells in a nonablated mouse model. Blood 89(11):4013–4020
Nilsson SK, Johnston HM et al (2001) Spatial localization of transplanted hemopoietic stem cells: inferences for the localization of stem cell niches. Blood 97(8):2293–2299
Kai T, Spradling A (2003) An empty Drosophila stem cell niche reactivates the proliferation of ectopic cells. Proc Natl Acad Sci USA 100(8):4633–4638
Fuchs E, Tumbar T et al (2004) Socializing with the neighbors: stem cells and their niche. Cell 116(6):769–778
Losick VP, Morris LX et al (2011) Drosophila stem cell niches: a decade of discovery suggests a unified view of stem cell regulation. Dev Cell 21(1):159–171
Dzierzak E, Speck NA (2008) Of lineage and legacy: the development of mammalian hematopoietic stem cells. Nat Immunol 9(2):129–136
Medvinsky A, Dzierzak E (1996) Definitive hematopoiesis is autonomously initiated by the AGM region. Cell 86(6):897–906
Dzierzak E (1999) Embryonic beginnings of definitive hematopoietic stem cells. Ann NY Acad Sci 872:256–262
Fliedner MC (2002) Research within the field of blood and marrow transplantation nursing: how can it contribute to higher quality of care? Int J Hematol 76(Suppl 2):289–291
Osawa M, Hanada K et al (1996) Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell. Science 273(5272):242–245
Suzuki N, Ohneda O et al (2006) Combinatorial Gata2 and Sca1 expression defines hematopoietic stem cells in the bone marrow niche. Proc Natl Acad Sci USA 103(7):2202–2207
Storb R, Graham TC et al (1977) Demonstration of hemopoietic stem cells in the peripheral blood of baboons by cross circulation. Blood 50(3):537–542
Wagers AJ, Sherwood RI et al (2002) Little evidence for developmental plasticity of adult hematopoietic stem cells. Science 297(5590):2256–2259
Wright DE, Wagers AJ et al (2001) Physiological migration of hematopoietic stem and progenitor cells. Science 294(5548):1933–1936
Lapid K, Itkin T et al (2013) GSK3beta regulates physiological migration of stem/progenitor cells via cytoskeletal rearrangement. J Clin Invest 123(4):1705–1717
Katayama Y, Battista M et al (2006) Signals from the sympathetic nervous system regulate hematopoietic stem cell egress from bone marrow. Cell 124(2):407–421
Mendez-Ferrer S, Lucas D et al (2008) Haematopoietic stem cell release is regulated by circadian oscillations. Nature 452(7186):442–447
Haylock DN, Williams B et al (2007) Hemopoietic stem cells with higher hemopoietic potential reside at the bone marrow endosteum. Stem Cells 25(4):1062–1069
Taichman RS, Emerson SG (1994) Human osteoblasts support hematopoiesis through the production of granulocyte colony-stimulating factor. J Exp Med 179(5):1677–1682
Taichman RS, Reilly MJ et al (1996) Human osteoblasts support human hematopoietic progenitor cells in vitro bone marrow cultures. Blood 87(2):518–524
Visnjic D, Kalajzic I et al (2001) Conditional ablation of the osteoblast lineage in Col2.3deltatk transgenic mice. J Bone Miner Res 16(12):2222–2231
Visnjic D, Kalajzic Z et al (2004) Hematopoiesis is severely altered in mice with an induced osteoblast deficiency. Blood 103(9):3258–3264
Calvi LM, Adams GB et al (2003) Osteoblastic cells regulate the haematopoietic stem cell niche. Nature 425(6960):841–846
Marusic A, Kalinowski JF et al (1993) Production of leukemia inhibitory factor mRNA and protein by malignant and immortalized bone cells. J Bone Miner Res 8(5):617–624
Arai F, Hirao A et al (2004) Tie2/angiopoietin-1 signaling regulates hematopoietic stem cell quiescence in the bone marrow niche. Cell 118(2):149–161
Jung Y, Wang J et al (2007) Annexin II expressed by osteoblasts and endothelial cells regulates stem cell adhesion, homing, and engraftment following transplantation. Blood 110(1):82–90
Qian H, Buza-Vidas N et al (2007) Critical role of thrombopoietin in maintaining adult quiescent hematopoietic stem cells. Cell Stem Cell 1(6):671–684
Weber JM, Forsythe SR et al (2006) Parathyroid hormone stimulates expression of the Notch ligand Jagged1 in osteoblastic cells. Bone 39(3):485–493
Yoshihara H, Arai F et al (2007) Thrombopoietin/MPL signaling regulates hematopoietic stem cell quiescence and interaction with the osteoblastic niche. Cell Stem Cell 1(6):685–697
Adams GB, Martin RP et al (2007) Therapeutic targeting of a stem cell niche (vol 25, pg 238, 2007). Nat Biotechnol 25(8):944–945
Bromberg O, Frisch BJ et al (2012) Osteoblastic N-cadherin is not required for microenvironmental support and regulation of hematopoietic stem and progenitor cells. Blood 120(2):303–313
Calvi LM, Sims NA et al (2001) Activated parathyroid hormone/parathyroid hormone–related protein receptor in osteoblastic cells differentially affects cortical and trabecular bone. J Clin Invest 107(3):277–286
Goltzman D (2008) Studies on the mechanisms of the skeletal anabolic action of endogenous and exogenous parathyroid hormone. Arch Biochem Biophys 473(2):218–224
Ballen KK, Shpall EJ et al (2007) Phase I trial of parathyroid hormone to facilitate stem cell mobilization. Biol Blood Marrow Transplant 13(7):838–843
Brunner S, Theiss HD et al (2007) Primary hyperparathyroidism is associated with increased circulating bone marrow-derived progenitor cells. Am J Physiol Endocrinol Metab 293(6):E1670–E1675
Lymperi S, Horwood N et al (2008) Strontium can increase some osteoblasts without increasing hematopoietic stem cells. Blood 111(3):1173–1181
Schepers K, Hsiao EC et al (2012) Activated Gs signaling in osteoblastic cells alters the hematopoietic stem cell niche in mice. Blood 120(17):3425–3435
Ma YD, Park C et al (2009) Defects in osteoblast function but no changes in long-term repopulating potential of hematopoietic stem cells in a mouse chronic inflammatory arthritis model. Blood 114(20):4402–4410
Nakamura Y, Arai F et al (2010) Isolation and characterization of endosteal niche cell populations that regulate hematopoietic stem cells. Blood 116(9):1422–1432
Cheng YH, Chitteti BR et al (2011) Impact of maturational status on the ability of osteoblasts to enhance the hematopoietic function of stem and progenitor cells. J Bone Miner Res 26(5):1111–1121
Chitteti BR, Cheng YH et al (2010) Impact of interactions of cellular components of the bone marrow microenvironment on hematopoietic stem and progenitor cell function. Blood 115(16):3239–3248
Chitteti BR, Cheng YH et al (2010) Osteoblast lineage cells expressing high levels of Runx2 enhance hematopoietic progenitor cell proliferation and function. J Cell Biochem 111(2):284–294
Calvi LM, Bromberg O et al (2012) Osteoblastic expansion induced by parathyroid hormone receptor signaling in murine osteocytes is not sufficient to increase hematopoietic stem cells. Blood 119(11):2489–2499
Xiao L, Liu P et al (2009) Exported 18-kDa isoform of fibroblast growth factor-2 is a critical determinant of bone mass in mice. J Biol Chem 284(5):3170–3182
Yoon KA, Cho HS et al (2012) Differential regulation of CXCL5 by FGF2 in osteoblastic and endothelial niche cells supports hematopoietic stem cell migration. Stem Cells Dev 21:3391–3402
Song X, Zhu CH et al (2002) Germline stem cells anchored by adherens junctions in the Drosophila ovary niches. Science 296(5574):1855–1857
Hosokawa K, Arai F et al (2010) Cadherin-based adhesion is a potential target for niche manipulation to protect hematopoietic stem cells in adult bone marrow. Cell Stem Cell 6(3):194–198
Hosokawa K, Arai F et al (2010) Knockdown of N-cadherin suppresses the long-term engraftment of hematopoietic stem cells. Blood 116(4):554–563
Levesque JP (2012) N(o)-cadherin role for HSCs. Blood 120(2):237–238
Dominici M, Rasini V et al (2009) Restoration and reversible expansion of the osteoblastic hematopoietic stem cell niche after marrow radioablation. Blood 114(11):2333–2343
Greenbaum AM, Revollo LD et al (2012) N-cadherin in osteolineage cells is not required for maintenance of hematopoietic stem cells. Blood 120(2):295–302
Frisch BJ, Porter RL et al (2009) In vivo prostaglandin E2 treatment alters the bone marrow microenvironment and preferentially expands short-term hematopoietic stem cells. Blood 114(19):4054–4063
Goessling W, Allen RS et al (2011) Prostaglandin E2 enhances human cord blood stem cell xenotransplants and shows long-term safety in preclinical nonhuman primate transplant models. Cell Stem Cell 8(4):445–458
North TE, Goessling W et al (2007) Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis. Nature 447(7147):1007–1011
Porter RL, Georger MA et al (2013) Prostaglandin E2 increases hematopoietic stem cell survival and accelerates hematopoietic recovery after radiation injury. Stem Cells 31(2):372–383
Sugimura R, He XC et al (2012) Noncanonical wnt signaling maintains hematopoietic stem cells in the niche. Cell 150(2):351–365
Bedi B, Li JY et al (2012) Silencing of parathyroid hormone (PTH) receptor 1 in T cells blunts the bone anabolic activity of PTH. Proc Natl Acad Sci USA 109(12):E725–E733
Tawfeek H, Bedi B et al (2010) Disruption of PTH receptor 1 in T cells protects against PTH-induced bone loss. PLoS One 5(8):e12290
Terauchi M, Li JY et al (2009) T lymphocytes amplify the anabolic activity of parathyroid hormone through Wnt10b signaling. Cell Metab 10(3):229–240
Li JY, Adams J et al (2012) PTH expands short-term murine hemopoietic stem cells through T cells. Blood 120(22):4352–4362
Fulciniti M, Tassone P et al (2009) Anti-DKK1 mAb (BHQ880) as a potential therapeutic agent for multiple myeloma. Blood 114(2):371–379
Qiang YW, Chen Y et al (2008) Myeloma-derived Dickkopf-1 disrupts Wnt-regulated osteoprotegerin and RANKL production by osteoblasts: a potential mechanism underlying osteolytic bone lesions in multiple myeloma. Blood 112(1):196–207
Vallet S, Pozzi S et al (2011) A novel role for CCL3 (MIP-1alpha) in myeloma-induced bone disease via osteocalcin downregulation and inhibition of osteoblast function. Leukemia 25(7):1174–1181
Colmone A, Amorim M et al (2008) Leukemic cells create bone marrow niches that disrupt the behavior of normal hematopoietic progenitor cells. Science 322(5909):1861–1865
Frisch BJ, Ashton JM et al (2012) Functional inhibition of osteoblastic cells in an in vivo mouse model of myeloid leukemia. Blood 119(2):540–550
Shiozawa Y, Pedersen EA et al (2011) Human prostate cancer metastases target the hematopoietic stem cell niche to establish footholds in mouse bone marrow. J Clin Invest 121(4):1298–1312
Simmons PJ, Torok-Storb B (1991) CD34 expression by stromal precursors in normal human adult bone marrow. Blood 78(11):2848–2853
Simmons PJ, Torok-Storb B (1991) Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood 78(1):55–62
Park D, Spencer JA et al (2012) Endogenous bone marrow MSCs are dynamic, fate-restricted participants in bone maintenance and regeneration. Cell Stem Cell 10(3):259–272
Masuda S, Ageyama N et al (2009) Cotransplantation with MSCs improves engraftment of HSCs after autologous intra-bone marrow transplantation in nonhuman primates. Exp Hematol 37(10):1250–1257
Ahn JY, Park G et al (2010) Intramarrow injection of beta-catenin-activated, but not naive mesenchymal stromal cells stimulates self-renewal of hematopoietic stem cells in bone marrow. Exp Mol Med 42(2):122–131
Sacchetti B, Funari A et al (2007) Self-renewing osteoprogenitors in bone marrow sinusoids can organize a hematopoietic microenvironment. Cell 131(2):324–336
McNiece I, Harrington J et al (2004) Ex vivo expansion of cord blood mononuclear cells on mesenchymal stem cells. Cytotherapy 6(4):311–317
Robinson SN, Ng J et al (2006) Superior ex vivo cord blood expansion following co-culture with bone marrow-derived mesenchymal stem cells. Bone Marrow Transplant 37(4):359–366
de Lima M, McNiece I et al (2012) Cord-blood engraftment with ex vivo mesenchymal-cell coculture. N Engl J Med 367(24):2305–2315
Pinho S, Lacombe J et al (2013) PDGFRα and CD51 mark human Nestin+ sphere-forming mesenchymal stem cells capable of hematopoietic progenitor cell expansion. J Exp Med 210:1351–1367
Nie Y, Han YC et al (2008) CXCR4 is required for the quiescence of primitive hematopoietic cells. J Exp Med 205(4):777–783
Tzeng YS, Li H et al (2011) Loss of Cxcl12/Sdf-1 in adult mice decreases the quiescent state of hematopoietic stem/progenitor cells and alters the pattern of hematopoietic regeneration after myelosuppression. Blood 117(2):429–439
Ara T, Itoi M et al (2003) A role of CXC chemokine ligand 12/stromal cell-derived factor-1/pre-B cell growth stimulating factor and its receptor CXCR4 in fetal and adult T cell development in vivo. J Immunol 170(9):4649–4655
Bonig H, Priestley GV et al (2004) PTX-sensitive signals in bone marrow homing of fetal and adult hematopoietic progenitor cells. Blood 104(8):2299–2306
Kawabata K, Ujikawa M et al (1999) A cell-autonomous requirement for CXCR4 in long-term lymphoid and myeloid reconstitution. Proc Natl Acad Sci USA 96(10):5663–5667
Peled A, Petit I et al (1999) Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4. Science 283(5403):845–848
Ding L, Morrison SJ (2013) Haematopoietic stem cells and early lymphoid progenitors occupy distinct bone marrow niches. Nature 495(7440):231–235
Greenbaum A, Hsu YM et al (2013) CXCL12 in early mesenchymal progenitors is required for haematopoietic stem-cell maintenance. Nature 495(7440):227–230
Ding L, Saunders TL et al (2012) Endothelial and perivascular cells maintain haematopoietic stem cells. Nature 481(7382):457–462
Chen MJ, Yokomizo T et al (2009) Runx1 is required for the endothelial to haematopoietic cell transition but not thereafter. Nature 457(7231):887–891
Kiel MJ, Yilmaz OH et al (2008) CD150– cells are transiently reconstituting multipotent progenitors with little or no stem cell activity. Blood 111(8):4413–4414 author reply 4414–4415
Butler JM, Nolan DJ et al (2010) Endothelial cells are essential for the self-renewal and repopulation of Notch-dependent hematopoietic stem cells. Cell Stem Cell 6(3):251–264
Chute JP, Muramoto GG et al (2006) Molecular profile and partial functional analysis of novel endothelial cell-derived growth factors that regulate hematopoiesis. Stem Cells 24(5):1315–1327
Kobayashi H, Butler JM et al (2010) Angiocrine factors from Akt-activated endothelial cells balance self-renewal and differentiation of haematopoietic stem cells. Nat Cell Biol 12(11):1046–1056
Hooper AT, Butler JM et al (2009) Engraftment and reconstitution of hematopoiesis is dependent on VEGFR2-mediated regeneration of sinusoidal endothelial cells. Cell Stem Cell 4(3):263–274
Winkler IG, Barbier V et al (2012) Vascular niche E-selectin regulates hematopoietic stem cell dormancy, self renewal and chemoresistance. Nat Med 18(11):1651–1657
Barker JE (1994) Sl/Sld hematopoietic progenitors are deficient in situ. Exp Hematol 22(2):174–177
Barker JE (1997) Early transplantation to a normal microenvironment prevents the development of Steel hematopoietic stem cell defects. Exp Hematol 25(6):542–547
Yamazaki S, Iwama A et al (2009) TGF-beta as a candidate bone marrow niche signal to induce hematopoietic stem cell hibernation. Blood 113(6):1250–1256
Yamazaki S, Ema H et al (2011) Nonmyelinating Schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche. Cell 147(5):1146–1158
Kirkland JL, Tchkonia T et al (2002) Adipogenesis and aging: does aging make fat go MAD? Exp Gerontol 37(6):757–767
Rosen CJ, Ackert-Bicknell C et al (2009) Marrow fat and the bone microenvironment: developmental, functional, and pathological implications. Crit Rev Eukaryot Gene Expr 19(2):109–124
Berkahn L, Keating A (2004) Hematopoiesis in the elderly. Hematology 9(3):159–163
Van Zant G, Liang Y (2012) Concise review: hematopoietic stem cell aging, life span, and transplantation. Stem Cells Transl Med 1(9):651–657
DiMascio L, Voermans C et al (2007) Identification of adiponectin as a novel hemopoietic stem cell growth factor. J Immunol 178(6):3511–3520
Berner HS, Lyngstadaas SP et al (2004) Adiponectin and its receptors are expressed in bone-forming cells. Bone 35(4):842–849
Naveiras O, Nardi V et al (2009) Bone-marrow adipocytes as negative regulators of the haematopoietic microenvironment. Nature 460(7252):259–263
Lymperi S, Ersek A et al (2011) Inhibition of osteoclast function reduces hematopoietic stem cell numbers in vivo. Blood 117(5):1540–1549
Mansour A, Abou-Ezzi G et al (2012) Osteoclasts promote the formation of hematopoietic stem cell niches in the bone marrow. J Exp Med 209(3):537–549
Adams GB, Chabner KT et al (2006) Stem cell engraftment at the endosteal niche is specified by the calcium-sensing receptor. Nature 439(7076):599–603
Christopher MJ, Liu F et al (2009) Suppression of CXCL12 production by bone marrow osteoblasts is a common and critical pathway for cytokine-induced mobilization. Blood 114(7):1331–1339
Levesque JP, Hendy J et al (2003) Disruption of the CXCR4/CXCL12 chemotactic interaction during hematopoietic stem cell mobilization induced by GCSF or cyclophosphamide. J Clin Invest 111(2):187–196
Petit I, Szyper-Kravitz M et al (2002) G-CSF induces stem cell mobilization by decreasing bone marrow SDF-1 and up-regulating CXCR4. [Erratum appears in Nat Immunol 2002;3(8):787]. Nat Immunol 3(7):687–694
Semerad CL, Christopher MJ et al (2005) G-CSF potently inhibits osteoblast activity and CXCL12 mRNA expression in the bone marrow. Blood 106(9):3020–3027
Kollet O, Dar A et al (2006) Osteoclasts degrade endosteal components and promote mobilization of hematopoietic progenitor cells. Nat Med 12(6):657–664
Miyamoto K, Yoshida S et al (2011) Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization. J Exp Med 208(11):2175–2181
Winkler IG, Barbier V et al (2010) Positioning of bone marrow hematopoietic and stromal cells relative to blood flow in vivo: serially reconstituting hematopoietic stem cells reside in distinct nonperfused niches. Blood 116(3):375–385
Winkler IG, Sims NA et al (2010) Bone marrow macrophages maintain hematopoietic stem cell (HSC) niches and their depletion mobilizes HSCs. Blood 116(23):4815–4828
Yona S, Kim KW et al (2013) Fate mapping reveals origins and dynamics of monocytes and tissue macrophages under homeostasis. Immunity 38(1):79–91
Alexander KA, Chang MK et al (2011) Osteal macrophages promote in vivo intramembranous bone healing in a mouse tibial injury model. J Bone Miner Res 26(7):1517–1532
Chow A, Lucas D et al (2011) Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche. J Exp Med 208(2):261–271
Ludin A, Itkin T et al (2012) Monocytes–macrophages that express alpha-smooth muscle actin preserve primitive hematopoietic cells in the bone marrow. Nat Immunol 13(11):1072–1082
Chow A, Huggins M et al (2013) CD169+ macrophages provide a niche promoting erythropoiesis under homeostasis and stress. Nat Med 19(4):429–436
Westerterp M, Gourion-Arsiquaud S et al (2012) Regulation of hematopoietic stem and progenitor cell mobilization by cholesterol efflux pathways. Cell Stem Cell 11(2):195–206
Liu F, Poursine-Laurent J et al (2000) Expression of the G-CSF receptor on hematopoietic progenitor cells is not required for their mobilization by G-CSF. Blood 95(10):3025–3031
Christopher MJ, Rao M et al (2011) Expression of the G-CSF receptor in monocytic cells is sufficient to mediate hematopoietic progenitor mobilization by G-CSF in mice. J Exp Med 208(2):251–260
Heissig B, Hattori K et al (2002) Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand. Cell 109(5):625–637
Levesque JP, Takamatsu Y et al (2001) Vascular cell adhesion molecule-1 (CD106) is cleaved by neutrophil proteases in the bone marrow following hematopoietic progenitor cell mobilization by granulocyte colony-stimulating factor. Blood 98(5):1289–1297
Levesque JP, Liu F et al (2004) Characterization of hematopoietic progenitor mobilization in protease-deficient mice. Blood 104(1):65–72
Singh P, Hu P et al (2012) Expansion of bone marrow neutrophils following G-CSF administration in mice results in osteolineage cell apoptosis and mobilization of hematopoietic stem and progenitor cells. Leukemia 26(11):2375–2383
Boneberg EM, Hareng L et al (2000) Human monocytes express functional receptors for granulocyte colony-stimulating factor that mediate suppression of monokines and interferon-gamma. Blood 95(1):270–276
Wang Y, Wan C et al (2007) The hypoxia-inducible factor alpha pathway couples angiogenesis to osteogenesis during skeletal development. J Clin Invest 117(6):1616–1626
Rankin EB, Wu C et al (2012) The HIF signaling pathway in osteoblasts directly modulates erythropoiesis through the production of EPO. Cell 149(1):63–74
Forristal CE, Winkler IG et al (2013) Pharmacologic stabilization of HIF-1alpha increases hematopoietic stem cell quiescence in vivo and accelerates blood recovery after severe irradiation. Blood 121(5):759–769
Takubo K, Goda N et al (2010) Regulation of the HIF-1alpha level is essential for hematopoietic stem cells. Cell Stem Cell 7(3):391–402
Levesque JP, Winkler IG et al (2007) Hematopoietic progenitor cell mobilization results in hypoxia with increased hypoxia-inducible transcription factor-1 alpha and vascular endothelial growth factor A in bone marrow. Stem Cells 25(8):1954–1965
Acknowledgements
The authors thank Dr. B. J. Frisch for review of the manuscript and members of the Calvi and Link laboratories for helpful discussions. This work is supported by the National Institutes of Health (NIDDK grants DK076876 and DK081843 to L. M. C. and HL60772 to D. C. L.).
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Calvi, L.M., Link, D.C. Cellular Complexity of the Bone Marrow Hematopoietic Stem Cell Niche. Calcif Tissue Int 94, 112–124 (2014). https://doi.org/10.1007/s00223-013-9805-8
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DOI: https://doi.org/10.1007/s00223-013-9805-8