Abstract
Peroxynitrite (ONOO−) and species derived from it can oxidize and nitrate lipids, proteins and DNA leading to changes in signaling molecules. The present study was carried out to elucidate possible effects of CNS peroxynitrite in a mouse model of orofacial pain. Mice that received facial carrageenan injection + intracerebroventricular (i.c.v.) injection of the peroxynitrite scavenger [5,10,15,20-Tetrakis (4-sulfonatophenyl) porphyrinato iron (III), chloride] (FeTPPS) showed significantly fewer face wash strokes upon probing the inflamed area of the face with a von Frey hair at 6 h after injection, compared to mice that received facial carrageenan alone, or facial carrageenan injection + i.c.v. injection of saline. Mice that received i.c.v. injection of FeTPPS without facial carrageenan injection showed no significant difference in response to von Frey hair stimulation, compared to mice that received i.c.v. injection of saline without facial carrageenan injection. These results indicate an anti-nociceptive effect of the peroxynitrite scavenger FeTPPS in carrageenan induced facial pain but no effect on normal tactile sensation and point to an important role of CNS peroxynitrite in nociception.
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References
Bessis A, Bechade C, Bernard D, Roumier A (2007) Microglial control of neuronal death and synaptic properties. Glia 55:233–238
Calabrese V, Mancuso C, Calvani M, Rizzarelli E, Butterfield DA, Stella AM (2007) Nitric oxide in the central nervous system: neuroprotection versus neurotoxicity. Nat Rev Neurosci 8:766–775
Davies SL, Loescher AR, Clayton NM, Bountra C, Robinson PP, Boissonade FM (2004) nNOS expression following inferior alveolar nerve injury in the ferret. Brain Res 1027:11–17
Fujita T, Kamisaki Y, Yonehara N (2004) Nitric oxide-induced increase of excitatory amino acid levels in the trigeminal nucleus caudalis of the rat with tactile hypersensitivity evoked by the loose-ligation of the inferior alveolar nerves. J Neurochem 91:558–567
Ischiropoulos H, al-Mehdi AB (1995) Peroxynitrite-mediated oxidative protein modifications. FEBS Lett 364:279–282
Leong S, Liu H, Yeo J (2000) Nitric oxide synthase and glutamate receptor immunoreactivity in the rat spinal trigeminal neurons expressing Fos protein after formalin injection. Brain Res 855:107–115
Obrosova IG, Mabley JG, Zsengeller Z, Charniauskaya T, Abatan OI, Groves JT, Szabo C (2005) Role for nitrosative stress in diabetic neuropathy: evidence from studies with a peroxynitrite decomposition catalyst. FASEB J 19:401–403
Possel H, Noack H, Putzke J, Wolf G, Sies H (2000) Selective upregulation of inducible nitric oxide synthase (iNOS) by lipopolysaccharide (LPS) and cytokines in microglia: in vitro and in vivo studies. Glia 32:51–59
Radi R, Beckman JS, Bush KM, Freeman BA (1991) Peroxynitrite-induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide. Arch Biochem Biophys 288:481–487
Salter MW (2005) Cellular signalling pathways of spinal pain neuroplasticity as targets for analgesic development. Curr Top Med Chem 5:557–567
Salvemini D, Wang ZQ, Stern MK, Currie MG, Misko TP (1998) Peroxynitrite decomposition catalysts: therapeutics for peroxynitrite-mediated pathology. Proc Natl Acad Sci USA 95:2659–2663
Spencer JP, Wong J, Jenner A, Aruoma OI, Cross CE, Halliwell B (1996) Base modification and strand breakage in isolated calf thymus DNA and in DNA from human skin epidermal keratinocytes exposed to peroxynitrite or 3-morpholinosydnonimine. Chem Res Toxicol 9:1152–1158
Szabo C, Ischiropoulos H, Radi R (2007) Peroxynitrite: biochemistry, pathophysiology and development of therapeutics. Nat Rev Drug Discov 6:662–680
Vos BP, Hans G, Adriaensen H (1998) Behavioral assessment of facial pain in rats: face grooming patterns after painful and non-painful sensory disturbances in the territory of the rat’s infraorbital nerve. Pain 76:173–178
Wang ZQ, Porreca F, Cuzzocrea S, Galen K, Lightfoot R, Masini E, Muscoli C, Mollace V, Ndengele M, Ischiropoulos H, Salvemini D (2004) A newly identified role for superoxide in inflammatory pain. J Pharmacol Exp Ther 309:869–878
Watkins LR, Martin D, Ulrich P, Tracey KJ, Maier SF (1997) Evidence for the involvement of spinal cord glia in subcutaneous formalin induced hyperalgesia in the rat. Pain 71:225–235
Wieseler-Frank J, Maier SF, Watkins LR (2004) Glial activation and pathological pain. Neurochem Int 45:389–395
Yeo JF, Liu HP, Leong SK (2001) Sustained microglial immunoreactivity in the caudal spinal trigeminal nucleus after formalin injection. J Dent Res 80:1524–1529
Yeo JF, Ong WY, Ling SF, Farooqui AA (2004) Intracerebroventricular injection of phospholipases A2 inhibitors modulates allodynia after facial carrageenan injection in mice. Pain 112:148–155
Yonehara N, Kudo C, Kamisaki Y (2003) Involvement of NMDA-nitric oxide pathways in the development of tactile hypersensitivity evoked by the loose-ligation of inferior alveolar nerves in rats. Brain Res 963:232–243
Acknowledgment
This work was supported by a grant from the National University of Singapore (R223-000-007-112). We thank Dr. S. K. Leong for initiating the project.
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Yeo, JF., Ling, SF., Tang, N. et al. Antinociceptive effect of CNS peroxynitrite scavenger in a mouse model of orofacial pain. Exp Brain Res 184, 435–438 (2008). https://doi.org/10.1007/s00221-007-1211-x
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DOI: https://doi.org/10.1007/s00221-007-1211-x