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Duplex digital droplet PCR for the determination of non-Basmati rice in Basmati rice (Oryza sativa) on the base of a deletion in the fragrant gene

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Abstract

Due to its higher trade value, a broad range of adulterations of Basmati rice with cheaper rice varieties can be found on the market. A duplex digital droplet PCR (ddPCR) assay was developed to test rice samples, labeled as “Basmati,” for their authenticity. The new ddPCR assay is based on the real-time PCR (rtPCR) method published previously. It showed to be able to screen for non-Basmati rice contents in the range of 1 % or higher. As digital PCR does not require calibrators for the quantification, the method is more economic, compared to rtPCR or microsatellite analysis (MA). It is also less time-consuming than MA and an alternative method for the verification of rtPCR results. Comparing results gained by all three methods revealed best correlation between ddPCR and MA.

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Acknowledgments

We thank the Food Control Authority of the Canton Zürich for providing the resources for this work.

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  1. Food Control Authority of the Canton Zürich, Zurich, Switzerland

    Thomas B. Bucher & René Köppel

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  1. Thomas B. Bucher
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  2. René Köppel
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Correspondence to René Köppel.

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Bucher, T.B., Köppel, R. Duplex digital droplet PCR for the determination of non-Basmati rice in Basmati rice (Oryza sativa) on the base of a deletion in the fragrant gene. Eur Food Res Technol 242, 927–934 (2016). https://doi.org/10.1007/s00217-015-2599-3

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  • DOI: https://doi.org/10.1007/s00217-015-2599-3

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