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β-Glucosidase removal due to bentonite fining during wine making

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Abstract

Grape proteins are discussed to cause haze formation in bottled wine leading to economic problems. A common way to remove these proteins is bentonite fining. Although the efficiency of bentonite treatment is well known, the underlying molecular mechanism of the fining process in wine is not well understood. Two white wine varieties, Riesling and Pinot gris, were treated in the must stage with different types of bentonite to investigate their efficiencies. In all cases, bentonite treatment significantly reduced the overall protein concentration based on SDS-PAGE analysis, albeit individual proteins were differently affected. However, our data indicate that one protein band was completely removed independent of the bentonite type. Mass spectrometry analysis identified the major protein as glucan endo-1,3-β-glucosidase of Vitis vinifera, which was accompanied by low amounts of several other proteins. Using molecular modeling, we propose that this enzyme with its positively charged surface is adsorbed on the negatively charged lamella of bentonite during the fining process.

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Abbreviations

DTT:

Dithiothreitol

ESI:

Electrospray ionization

LC–MS/MS:

Liquid chromatography–mass spectroscopy

SDS:

Sodium dodecyl sulfate

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

TLP:

Thaumatin-like protein

TOF:

Time-of-flight

TRIS:

Tris(hydroxymethyl)aminomethane

UPLC:

Ultra-performance liquid chromatography

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Acknowledgments

This work was granted by the Foundation Rhineland-Palatinate for Innovation (Grant number 961-386261/929) and the Federal Ministry for Economic Affairs and Energy (project AiF 17338 N).

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The authors declare that they have no conflict of interest.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Correspondence to Nadine Jaeckels or Heinz Decker.

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Jaeckels, N., Tenzer, S., Rosch, A. et al. β-Glucosidase removal due to bentonite fining during wine making. Eur Food Res Technol 241, 253–262 (2015). https://doi.org/10.1007/s00217-015-2451-9

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  • DOI: https://doi.org/10.1007/s00217-015-2451-9

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