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Genetic system for traceability of goatfishes by FINS methodology and authentication of mullets (Mullus barbatus and Mullus surmuletus) by RT-PCR

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Abstract

In the present study, two methods for the identification of goatfishes using the cytochrome b gene fragment were developed. The first method is based on the phylogenetic analysis of DNA sequences (forensically informative nucleotide sequencing), which allows the unequivocal identification of all goatfish species included in this study, while the second one is based on TaqMan probe RT-PCR technology for the authentication of Mullus barbatus and Mullus surmuletus. The methods were checked for the manufacturing process of food, reflecting that it had no influence on the identification of goatfishes. Both techniques can be applied depending on the laboratory equipment and allow the detection of fraudulent or unintentional mislabeling of these species. These tools are useful to clarify questions related to the correct labeling of commercial products and to verify the correct traceability in commercial trade and for fisheries control.

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Acknowledgments

We thank Fátima C. Lago and Mercedes Alonso for their helpful assistance and Avelino Dominguez for their bibliographic review. We also thank Giuseppe Arcangeli [Instituto Zooprofilattico Sperimentale delle Venezie (IZSV)], Carla Giansante (Instituto Zooprofilattico Speromentale Dell’Abruzzo e Del Molise), Marco Arculeo (Dipartimento STEBICEF de Biologia Ambientale e Biodiversita, UNIPA, Italia), Gioacchino Bono (Istituto per l’ Ambiente Marino Costiero, Consiglio Nazionale delle Ricerche, Italy), Nir Stern (George S. Wise Faculty of Life Science, Department of Zoology, Tel Aviv University, Israel), Chiara Motta (Department of Biology, Univ. Napoli, Napoli, Italia), Vasiliki Kousteni 8IMBG, Hellenic Centre for Marine Research (HCMR), Grecia and Mark Erdmann (Conservation International, Bali, Indonesia), for providing some of the samples included in this work.

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This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Research Division of Living Resources, Aquaculture and Traceability Management, ANFACO-CECOPESCA, 36310, Vigo, Pontevedra, Spain

    Montserrat Espiñeira & Juan M. Vieites

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  1. Montserrat Espiñeira
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  2. Juan M. Vieites
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Correspondence to Montserrat Espiñeira.

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Espiñeira, M., Vieites, J.M. Genetic system for traceability of goatfishes by FINS methodology and authentication of mullets (Mullus barbatus and Mullus surmuletus) by RT-PCR. Eur Food Res Technol 240, 423–429 (2015). https://doi.org/10.1007/s00217-014-2341-6

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  • DOI: https://doi.org/10.1007/s00217-014-2341-6

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