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Determination of microheterogeneous substitution in shrimp tropomyosin and its effect on IgE-binding capacity

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Abstract

Tropomyosin (TM), the major shrimp allergen that belongs to a highly conserved protein family, has frequently been investigated owing to its high rate of human consumption. In this study, the sequence microheterogeneity of TMs, irrespective of the organism and differences in the N- and C-termini, from different shrimp species was analyzed. The TM sequences were analyzed using bioinformatics tools and confirmed by dot-blot using human serum. The results showed that all 13 shrimp species share high mutual TM sequence identity values, ranging from 85.5 to 99.7 %, and 60 cases of sequence microheterogeneity were identified in shrimp TM, with a sequence identity ranging from 90.1 to 99.7 %. A total of 719 unique amino acid residue substitutions (that affect 43 residues, 15 % of 284 residues) involving sequence microheterogeneity were characterized, in which 214 substitutions involving 15 residues were located in the epitope regions. About 45 % of these substitutions occurred in the TM sequences that are reported allergens, whereas 43 % of the substitutions occurred overall for the allergenic and nonallergenic TMs. Dot-blot immunoassay revealed that single microheterogeneous mutation of allergic peptides resulted in significant changes in the IgE-binding capacity of the peptides. Overall, microheterogeneous mutations of TM occurred in different shrimp species. These mutations can potentially induce changes in the IgE-binding capacity and might be responsible for variations in the hypersensitivity to different shrimp species.

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Acknowledgments

The authors thank Dr. Chen Guan-zhi from Qingdao Medical College for initiating studies on the prevalence of seafood allergies in China. This work was supported by National Natural Science Foundation of China (Grants 31371730) and Program for Changjiang Scholars and Innovative Research Team in University.

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None.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, People’s Republic of China

    RongFang Huang, ZhenXing Li, Hong Lin & Muhammad Naseem Khan

  2. Food and Marine Resources Research Center, PCSIR Laboratories Complex, Karachi, Pakistan

    Muhammad Naseem Khan

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  1. RongFang Huang
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  2. ZhenXing Li
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  3. Hong Lin
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Correspondence to ZhenXing Li.

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Huang, R., Li, Z., Lin, H. et al. Determination of microheterogeneous substitution in shrimp tropomyosin and its effect on IgE-binding capacity. Eur Food Res Technol 239, 941–949 (2014). https://doi.org/10.1007/s00217-014-2291-z

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  • DOI: https://doi.org/10.1007/s00217-014-2291-z

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