Abstract
Lysozyme is an important component of egg white with antibacterial properties especially against Gram-positive bacteria. Research indicates that the range of lysozyme activity may be extended thanks to chemical or physical modifications. The purpose of the study was to determine the effectiveness of antibacterial action against selected strains of bacteria of lysozyme modified by the thermochemical technique. Our investigations indicated a possibility of extending the range of lysozyme activity using modification. We found that under the adopted conditions modification promotes an increased polymerisation of lysozyme. It was observed that the temperature and time of modification have an effect on the content of the forming polymers. It was found that the application of lysozyme modification leads to an increase in its antibacterial activity in comparison to the activity of lysozyme monomer. The experiments have shown that the used lysozyme preparations demonstrate different activity in relation to different types of bacteria. The observed increase in antibacterial activity of modified lysozyme preparations against Gram-negative bacteria were not associated with a decrease of that activity against Gram-positive bacteria. The greatest reduction of growth of the examined Gram-negative bacteria species was recorded for the preparation which contained the highest quantity of dimer (34–37.6%). A more effective action against bacteria Staphylococcus epidermidis was observed for preparations containing 25–27% of dimer.
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References
Baron F, Réhault S (2007) Compounds with antibacterial activity. In: Huophalathi R, Lopez-Fandiño R, Anton M, Schade R (eds) Bioactive egg compounds. Springer, Berlin, pp 191–198
Carneiro de Melo AMS, Cassar CA, Miles RJ (1998) J Food Prot 61:839–844
Cegielska-Radziejewska R, Lesnierowski G, Kijowski J (2003) In: Proceedings of 10th European symposium on the quality of eggs and egg products. Saint-Brieuc, France, pp 380–387
Düring K, Porsch P, Mahn A, Brinkmann O, Gieffers W (1999) FEBS Lett 449:93–100
Gill AO, Holley RA (2000) Food Res Int 33:83–90
Hughey VL, Johnson EH (1987) Appl Environ Microbiol 53:2165–2170
Ibrahim HR (2003) In: Proceedings of the 10th European symposium on the quality of eggs and egg products. Saint-Brieuc, France, pp 350–365
Ibrahim HR, Kato A, Kobayashi K (1991) J Agric Food Chem 39:2077–2082
Ibrahim HR, Kobayashi K, Kato A (1993) J Agric Food Chem 41:1164–1168
Ibrahim HR, Yamada M, Matsushita K, Kobayashi K, Kato A (1994) J Biol Chem 269:5059–5063
Ibrahim HR, Higashiguchi S, Juneja LR, Kim M, Yamamoto T (1996) J Agric Food Chem 44:1416–1423
Ibrahim HR, Higashiguchi S, Sugimoto Y, Aoki T (1997) J Agric Food Chem 45:89–94
Ibrahim HR, Matsuzaki T, Aoki T (2001) FEBS Lett 506:27–32
Ibrahim HR, Aoki T, Pellegrini A (2002) Curr Pharm Des 8:672–693
Johnson EA (1994) Egg-white lysozyme as a preservative for use in foods. In: Sim JS, Nakai S (eds) Egg uses and processing technologies new developments. CAB International, Wallingford, pp 177–191
Kato A, Nakamura S, Ibrahim H, Matsumi T, Tsumiyama C, Kato M (1998) Nahrung 42:128–130
Kijowski J, Lesnierowski G, Fabisz-Kijowska A (2000) Lysozyme polymer formation and functionality of residuals after lysozyme extraction. In: Sim JS, Nakai S, Guenter W (eds) Egg nutrition and biotechnology. CAB International, Wallingford, pp 269–285
Kijowski J, Marciszewska C, Cegielska-Radziejewska R (2002) Pol J Food Nutr Sci 2:47–54
Laemmli UK (1970) Nature 227:680–685
Lesnierowski G (1997) Isolation of lysozyme from hen egg white using the crystallization, ultrafiltration and ion exchange methods. Dissertation, Agricultural University of Poznań (in Polish), pp 1–118
Lesnierowski G, Kijowski J (1995) Przem Spoż 12:476–479 (in Polish)
Lesnierowski G, Kijowski J (2000) Żywność 2(23):59–69 (in Polish, abstract in English)
Lesnierowski G, Kijowski J (2001) P J Food Nutr Sci 2:43–47
Lesnierowski G, Kijowski J (2007) Lysozyme. In: Huophalathi R, Lopez-Fandiño R, Anton M, Schade R (eds) Bioactive egg compounds. Springer, Berlin, pp 33–42
Lesnierowski G, Cegielska-Radziejewska R, Kijowski J (2004) Worlds Poult Sci J 60(3):303–310
Masschalck B, Van Houdt R, Van Haver EGR, Michiels CW (2001) Appl Environ Microbiol 67:339–344
Proctor VA, Cunningham FE (1988) CRC Crit Rev Food Sci Nutr 26:359–395
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This study was supported by the State Committee for Scientific Research within project no. 2PO6T00926.
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Cegielska-Radziejewska, R., Lesnierowski, G. & Kijowski, J. Antibacterial activity of hen egg white lysozyme modified by thermochemical technique. Eur Food Res Technol 228, 841–845 (2009). https://doi.org/10.1007/s00217-008-0997-5
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DOI: https://doi.org/10.1007/s00217-008-0997-5