Abstract
A reproducible and sensitive HPLC assay with fluorometric detection for measuring malondialdehyde as an index of lipid peroxidation in dry sausage (salami) has been developed. Following the homogenisation of dry sausage in water, a solution of 5% ice-cooled trichloroacetic acid led to the precipitation of the proteins. The addition of thiobarbituric acid (TBA) (0.02 M) to the filtered mixture and its corresponding incubation (35 min, 100 °C) completed the method. High correlation coefficients (r>0.977), with regard to the distillative TBA test, were obtained. Ratios between the HPLC and the distillation method of 2.23 were found. Also, the effect of the antioxidant BHT and an examination of the distillative classic TBA test have been done. Results indicate that this HPLC method, on account of its simplicity, sensitivity and specificity, may be preferred over the classic TBA test for estimating the rancidity of dry sausage.
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References
Sinnhuber R, Yu TC (1958) Food Technol 12:9–12
Tarladgis BG, Watts BM, Younathan MT, Dugan L (1960) J Am Oil Chem Soc 37:44–48
Kosugi H, Kato T, Kikugawa K (1987) Anal Biochem 165:456–464
Kosugi H, Kato T, Kikugawa K (1988) Lipids 23:1024–1031
Kosugi H, Kojima T, Kikugawa K (1989) Lipids 24:873–881
Janero DR (1990) Free Radical Biol Med 9:515–540
Shamberger RJ, Shamberger BA, Willis CE (1977) J Nutr 107:1404–1409
Squires EJ (1990) Poult Sci 69:1371–1376
Kakuda Y, Stanley DW, Voort FR (1981) J Am Oil Chem Soc 58:773–775
Tsaknis J, Lalas S, Evmorfopoulos E (1999) Analyst 124:843–845
Bergamo P, Fedele E, Balestrieri M, Abrescia P, Ferrara L (1998) J Agric Food Chem 46:2171–2176
Jo C, Ahn DU (1998) Poult Sci 77:475–480
Zipser MW, Watts B W (1962) Food Technol 16:102–104
Kwon T, Menzel DB, Olcott HS (1965) J Food Sci 30:808–813
Draper HH, McGirr LG, Hadley M (1986) Lipids 21:305–307
Botsoglou NA, Fletouris DJ, Papageorgiou GE, Vassilopoulos VN, Mantis AJ, Trakatellis AG (1994) J Agric Food Chem 42:1931–1937
Wang C, Zhu L, Brewer MS (1997) J Food Lipids 4:87–96
Pikul J, Leszcezynski DE, Kummerow FA (1989) J Agric Food Chem 37:1309–1313
Raharjo S, Sofos JN, Schmidt GR (1993) J Food Sci 58:921–924
Siu GM, Draper HH (1978) J Food Sci 43:1147–1149
Rhee K (1978) J Food Sci 43:1776–1779
Pikul J, Leszczynski DE, Kummerow FA (1989) J Agric Food Chem 37:1309–1313
Ke PJ, Cervantes E, Robles-Martinez C (1984) J Sci Food Agric 35:1248–1254
Salih AM, Smith DM, Dawson LE (1987) Poult Sci 66:1483–1488
Salih AM, Price JF, Smith DM, Dawson LE (1989) Poult Sci 68:754–761
Caldironi HA, Bazan NG (1982) J Food Sci 47:1329–1332, 1337
Spanier AM, Traylor RD (1991) J Muscle Foods 2:165–175
Moerck KE, Ball HR (1974) J Food Sci 39:876–879
Shahidi F, Hong C (1991) J Food Biochem 15:97–105
Hoyland DV, Taylor AJ (1991) Food Chem 40:271–291
Karastogiannidou C, Ryley J (1994) Int J Food Sci Technol 29:19–22
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de las Heras, A., Schoch, A., Gibis, M. et al. Comparison of methods for determining malondialdehyde in dry sausage by HPLC and the classic TBA test. Eur Food Res Technol 217, 180–184 (2003). https://doi.org/10.1007/s00217-003-0728-x
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DOI: https://doi.org/10.1007/s00217-003-0728-x