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Novel concept of washing for microfluidic paper-based analytical devices based on capillary force of paper substrates

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Abstract

A novel washing technique for microfluidic paper-based analytical devices (μPADs) that is based on the spontaneous capillary action of paper and eliminates unbound antigen and antibody in a sandwich immunoassay is reported. Liquids can flow through a porous medium (such as paper) in the absence of external pressure as a result of capillary action. Uniform results were achieved when washing a paper substrate in a PDMS holder which was integrated with a cartridge absorber acting as a porous medium. Our study demonstrated that applying this washing technique would allow μPADs to become the least expensive microfluidic device platform with high reproducibility and sensitivity. In a model μPAD assay that utilized this novel washing technique, C-reactive protein (CRP) was detected with a limit of detection (LOD) of 5 μg mL−1.

A novel washing technique for microfluidic paper-based analytical devices (μPADs) that is based on the spontaneous capillary action of paper and eliminates unbound antigen and antibody in a sandwich immunoassay is reported

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Acknowledgments

This research was partially supported by the Urakami Foundation for Food and Food Culture Promotion.

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Authors and Affiliations

  1. Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo, 060-8628, Japan

    Saeed Mohammadi & Lori Shayne Alamo Busa

  2. Physical Sciences Department, College of Art and Sciences, Nueva Vizcaya State University, Bayombong, Nueva Vizcaya, 3700, Philippines

    Lori Shayne Alamo Busa

  3. Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo, 060-8628, Japan

    Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani & Manabu Tokeshi

  4. Department of Pharmaceutical Science, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario, M5S 3M2, Canada

    Reza M. Mohamadi

  5. ImPACT Research Centre for Advanced Nanobiodevices, Nagoya University, Furo-cho Chikusa-ku, Nagoya, 464-8603, Japan

    Manabu Tokeshi

  6. Innovative Research Centre for Preventive Medical Engineering, Nagoya University, Furo-cho Chikusa-ku, Nagoya, 464-8603, Japan

    Manabu Tokeshi

  7. Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan

    Manabu Tokeshi

Authors
  1. Saeed Mohammadi
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  2. Lori Shayne Alamo Busa
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  3. Masatoshi Maeki
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  4. Reza M. Mohamadi
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  5. Akihiko Ishida
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  6. Hirofumi Tani
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  7. Manabu Tokeshi
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Correspondence to Manabu Tokeshi.

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Mohammadi, S., Busa, L.S.A., Maeki, M. et al. Novel concept of washing for microfluidic paper-based analytical devices based on capillary force of paper substrates. Anal Bioanal Chem 408, 7559–7563 (2016). https://doi.org/10.1007/s00216-016-9853-9

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  • DOI: https://doi.org/10.1007/s00216-016-9853-9

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