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Molecularly imprinted polymer as efficient sorbent of solid-phase extraction for determination of gonyautoxin 1,4 in seawater followed by high-performance liquid chromatography-fluorescence detection

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Abstract

A kind of new molecularly imprinted polymer (MIP) was synthesized by bulk polymerization using guanosine as dummy template molecule, α-methacrylic acid as functional monomer and ethylene glycol dimethyl acrylic ester as crosslinker. Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) showed that the MIP had homogenous and uniform-sized cavities. It was confirmed that the MIP had higher binding affinity and selectivity towards gonyautoxins 1,4 (GTX 1,4) than the non-imprinted polymer (NIP) according to the static equilibrium adsorption. An off-line molecularly imprinted solid-phase extraction (MISPE) method followed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was established for the analysis of GTX 1,4. 0.1 mol/L acetic acid and 95:5 (v:v) methanol/water were optimized as the washing and elution solutions, respectively. The recoveries of spiked cultured seawater samples were satisfactory, as high as 88 %. Using this method, the concentrations of GTX 1,4 from cultured seawater samples of Alexandrium minutum and Alexandrium tamarense were detected to be 1.10 μg/L and 0.99 μg/L, respectively.

The synthesis of molecularly imprinted polymer and molecularly imprinted solid-phase extraction analysis for gonyautoxin 1,4

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Acknowledgments

This work was supported by National Programme on Global Change and Air-Sea Interaction under Grant number GASI-03-01-02-01 and the Public Science and Technology Research Funds Projects of Ocean under Grant number 201505034.

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Authors and Affiliations

  1. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, 238 Songling Road, Qingdao, Shandong, 266100, China

    Xiao-Qi Mei, Xiu-Ping He & Jiang-Tao Wang

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  1. Xiao-Qi Mei
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  2. Xiu-Ping He
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  3. Jiang-Tao Wang
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Correspondence to Jiang-Tao Wang.

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Mei, XQ., He, XP. & Wang, JT. Molecularly imprinted polymer as efficient sorbent of solid-phase extraction for determination of gonyautoxin 1,4 in seawater followed by high-performance liquid chromatography-fluorescence detection. Anal Bioanal Chem 408, 5737–5743 (2016). https://doi.org/10.1007/s00216-016-9675-9

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  • DOI: https://doi.org/10.1007/s00216-016-9675-9

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