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Rapid evaluation of artesunate quality with a specific monoclonal antibody-based lateral flow dipstick

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Abstract

Artesunate is a frontline antimalarial drug for treating Plasmodium falciparum malaria. To produce specific antibodies to artesunate, the carboxyl group of artesunate was directly conjugated to carrier protein as the immunogen. A specific monoclonal antibody (mAb) 3D82G6 against artesunate was obtained by high-throughput screening of positive hybridoma clones. This monoclonal antibody had 4.0, 0.5, and 0.9 % cross reactivities with artemisinin, dihydroartemisinin, and artemether, respectively. A dipstick immunoassay was developed, and the indicator range for artesunate was 1000–2000 ng mL−1. No interference was observed with artemisinin, dihydroartemisinin, artemether, and other commonly used antimalarial drugs for up to 20,000 ng mL−1. The dipsticks were used for determination of artesunate contents in commercial drugs, and the results were agreeable with those determined by high-performance liquid chromatography. This dipstick, with its specificity and sensitivity for artesunate and simplicity to use, makes it a potential point-of-care device for rapid quality evaluation of artesunate-containing antimalarial drugs.

Specific monoclonal antibody-based lateral flow dipstick for artesunate

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Acknowledgments

This research was supported by National Institute of Allergy and Infectious Diseases, National Institutes of Health (U19AI089672).

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Authors and Affiliations

  1. College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China

    Suqin Guo, Wei Zhang, Lishan He, Guiyu Tan & Baomin Wang

  2. Myanmar Medical Association, No. 249, Thein Phyu Road, Mingalartaungnyunt Township, Yangon, Myanmar

    Myo Min

  3. Department of Medical Research, Ministry of Health, No. 5, Ziwaka Road, Dagon Township, Yangon, Myanmar

    Myat Phone Kyaw

  4. Department of Entomology, Pennsylvania State University, University Park, PA, 16802, USA

    Liwang Cui

Authors
  1. Suqin Guo
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  2. Wei Zhang
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  3. Lishan He
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  4. Guiyu Tan
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  5. Myo Min
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  6. Myat Phone Kyaw
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  7. Baomin Wang
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  8. Liwang Cui
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Corresponding authors

Correspondence to Baomin Wang or Liwang Cui.

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Animal treatment

The authors declare that they have no competing interests.

Ethical standards

Female Balb/c mice were purchased from the Laboratory Animal Center of the Institute of Genetics (Beijing, China). This study was performed strictly according to the standards described in the “Guide for the Care and Use of Laboratory Animals” (National Research Council Commission on Life Sciences, 1996 edition). All animal treatment procedures were approved by the Animal Care Committee of China Agricultural University (CAU), and all efforts were made to minimize suffering. Mice were housed under controlled temperature (22 ± 2 °C) and lighting (12 h light/12 h darkness) with food and water ad libitum in air-conditioned rooms. All experimental mice were killed by cervical dislocation.

Additional information

Published in the topical collection Immunoanalysis for Environmental Monitoring and Human Health with guest editors Shirley J. Gee, Ivan R. Kennedy, Alice Lee, Hideo Ohkawa, Tippawan Prapamontol, and Ting Xu.

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Guo, S., Zhang, W., He, L. et al. Rapid evaluation of artesunate quality with a specific monoclonal antibody-based lateral flow dipstick. Anal Bioanal Chem 408, 6003–6008 (2016). https://doi.org/10.1007/s00216-016-9363-9

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  • DOI: https://doi.org/10.1007/s00216-016-9363-9

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