Skip to main content

Advertisement

SpringerLink
Log in

MTB-DR-RIF 9G membrane: a platform for multiplex SNP detection of multidrug-resistant TB

  • Research Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

The MTB-DR-RIF 9G membrane can detect by detecting multiple mutations in multiple codons. The MTB-DR-RIF 9G membrane possesses clinical applicability in point-of-care settings for the following reasons: (i) 100 % similar results with that of the sequencing analysis for clinical samples, (ii) discrimination of the multiple mutations in multiple codons, (iii) a specific/non-specific hybridization ratio higher than 350:1, and (iv) the sensitivity was found to be 1–10 copies/test for detection and discrimination of the wild and mutant TB strains.

Schematic illustration of the effect of controller DNA on the hybridization of the immobilized probes (corresponding to the wild TB strain) with the PCR product of (a) wild TB strain and (b) mutant TB strain

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. World Health Organization (WHO) (2013) Global tuberculosis report 2013. Report WHO/HTM/TB/2013.11. World Health Organization, Geneva, Switzerland

  2. World Health Organization (WHO) (2012) Global tuberculosis report 2012. Report WHO/H TM/T B/2012.6. World Health Organization, Geneva, Switzerland

  3. Boehme CC, Saacks S, O’Brienet RJ (2013) Semin Respir Crit Care Med 34:17–31

    Article  Google Scholar 

  4. Wang H, Liu W, Wu Z, Tang L, Xu X, Yu R, Jiang J (2011) Anal Chem 83:1883–1889

    Article  CAS  Google Scholar 

  5. Horne DJ, Pinto LM, Arentz M, Lin S-YG, Desmond E, Flores LL, Steingart KR, Minion J (2013) J Clin Microbiol 51:393–401

    Article  CAS  Google Scholar 

  6. Shah NS, Richardson J, Moodley P, Moodley S, Babaria P, Ramtahal M, Heysell SK, Li X, Moll AP, Friedland G, Sturm AW, Gandhi NR (2011) Emerg Infect Dis 17:510–513

    Article  Google Scholar 

  7. Boehme CC, Nabeta P, Hillemann D, Nicol MP, Shenai S, Krapp F, Allen J, Tahirli R, Blakemore R, Rustomjee R, Milovic A, Jones M, O’Brien SM, Persing DH, Ruesch-Gerdes S, Gotuzzo E, Rodrigues C, Alland D, Perkins MD (2010) N Engl J Med 363:1005–1015

    Article  CAS  Google Scholar 

  8. Lawn SD, Mwaba P, Bates M, Piatek A, Alexander H, Marais BJ, Cuevas LE, McHugh TD, Zijenah L, Kapata N, Abubakar I, McNerney R, Hoelscher M, Memish ZA, Migliori GB, Kim P, Maeurer M, Schito M, Zumla A (2013) Lancet Infect Dis 13:349–361

    Article  CAS  Google Scholar 

  9. Zhang Y, Guo Y, Quirke P, Zhou D (2013) Nanoscale 5:5027–5035

    Article  CAS  Google Scholar 

  10. Song K, Nimse SB, Kim J, Kim J, Nguyen V, Ta V, Kim T (2011) Chem Commun 47:7101–7103

    Article  CAS  Google Scholar 

  11. Nimse SB, Kim J, Ta V, Kim H, Song K, Jung C, Nguyen V, Kim T (2009) Tetrahedron Lett 50:7346–7350

    Article  CAS  Google Scholar 

  12. An H, Song K, Nimse SB, Kim J, Nguyen V, Ta V, Sayyed DR, Kim T (2012) J Clin Microbiol 50:562–568

    Article  CAS  Google Scholar 

  13. Nimse SB, Nguyen V, Kim J, Kim H, Song K, Eoum W, Jung C, Ta V, Seelam SR, Kim T (2010) Tetrahedron Lett 51:2840–2845

    Article  CAS  Google Scholar 

  14. Sayyed DR, Nimse SB, Song K, Kim T (2014) Chem Commun 50:12344–12347

    Article  CAS  Google Scholar 

  15. Wang J, Lee L, Chou C, Huang C, Wang S, Lai H, Hsueh P, Luh K (2004) J Clin Microbiol 42:4599–4603

    Article  CAS  Google Scholar 

  16. Song K, Nimse SB, Kim J, Sayyed DR, Kim T (2013) Chem Commun 49:2661–2663

    Article  CAS  Google Scholar 

  17. Nimse SB, Kim T (2013) Chem Soc Rev 42:366–386

    Article  CAS  Google Scholar 

  18. Nimse SB, Song K, Kim J, Kim H, Nguyen V, Eoum W, Jung C, Ta V, Kim T (2010) Tetrahedron Lett 51:6156–6160

    Article  CAS  Google Scholar 

  19. Zhu L, Zhang Z, Liang D, Jiang D, Wang C, Du N, Zhang Q, Mitchelson K, Cheng J (2007) Antimicrob Agents Chemoth 51:3707–3713

    Article  CAS  Google Scholar 

  20. Sayyed DR, Nimse SB, Song K, Sung N, Kim T (2014) Chem Commun 50:14585–14588

    Article  CAS  Google Scholar 

  21. Nimse SB, Song K, Kim J, Ta V, Nguyen V, Kim T (2011) Chem Commun 47:12444–12446

    Article  CAS  Google Scholar 

  22. Tang X et al (2006) BioTechniques 40:759–763

    Article  CAS  Google Scholar 

  23. Deng M, Feng S, Luo F, Wang S, Sun X, Zhou X, Zhang X (2012) J Clin Microbiol 50:3443–3450

    Article  CAS  Google Scholar 

  24. Shen W, Lim CL, Gao Z (2013) Chem Commun 49:8114–8116

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (Project No.: HI13C0858).

Conflict of interest

The authors declare that they have no competing interests.

Author information

Author notes

    Authors and Affiliations

    1. Institute for Applied Chemistry and Department of Chemistry, Hallym University, 1 Okcheon-dong, Chuncheon, 200-702, South Korea

      Danishmalik Rafiq Sayyed, Satish Balasaheb Nimse & Taisun Kim

    2. Biometrix Technology, Inc, 202 BioVenture Plaza, Chuncheon, 200-161, South Korea

      Keum-Soo Song

    3. Clinical Research Center, National Masan Hospital, Masan, South Korea

      Nackmoon Sung

    Authors
    1. Danishmalik Rafiq Sayyed
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Satish Balasaheb Nimse
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Keum-Soo Song
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Nackmoon Sung
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Taisun Kim
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Taisun Kim.

    Additional information

    Danishmalik Rafiq Sayyed and Satish Balasaheb Nimse contributed equally to this work.

    Electronic supplementary material

    Below is the link to the electronic supplementary material.

    ESM 1

    (PDF 2477 kb)

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Sayyed, D.R., Nimse, S.B., Song, KS. et al. MTB-DR-RIF 9G membrane: a platform for multiplex SNP detection of multidrug-resistant TB. Anal Bioanal Chem 407, 5739–5745 (2015). https://doi.org/10.1007/s00216-015-8754-7

    Download citation

    • Received:

    • Revised:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s00216-015-8754-7

    Keywords

    Search

    Navigation