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Surface-enhanced Raman spectroscopy of urine for prostate cancer detection: a preliminary study

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Abstract

Surface-enhanced Raman scattering (SERS) spectra were obtained from urine samples from subjects diagnosed with prostate cancer as well as from healthy controls, using Au nanoparticles as substrates. Principal component analysis (PCA) of the spectral data, followed by linear discriminant analysis (LDA), leads to a classification model with a sensitivity of 100 %, a specificity of 89 %, and an overall diagnostic accuracy of 95 %. Even considering the very limited number of samples involved in this report, preliminary results from this approach are extremely promising, encouraging further investigation.

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Acknowledgments

We would like to thank Dr. Sergio Lenarduzzi for coordinating sample collection at Policlinico San Giorgio (Pordenone, Italy). AB would like to thank C. Beleites for the useful discussions on LDA.

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Authors and Affiliations

  1. Department of Engineering and Architecture, University of Trieste, Via Valerio 6a, 34127, Trieste, TS, Italy

    Greta Del Mistro, Elena Mansutti, Valter Sergo & Alois Bonifacio

  2. CRO-Biobank, CRO Aviano, National Cancer Institute, Via Franco Gallini 2, 33081, Aviano, PN, Italy

    Silvia Cervo, Elena Mansutti & Agostino Steffan

  3. Clinical Cancer Pathology, CRO Aviano, National Cancer Institute, Via Franco Gallini 2, 33081, Aviano, PN, Italy

    Silvia Cervo & Agostino Steffan

  4. Division of Experimental Oncology 2, CRO Aviano, National Cancer Institute, Via Franco Gallini 2, 33081, Aviano, PN, Italy

    Riccardo Spizzo & Alfonso Colombatti

  5. Centre of Excellence MAT Department of Medical and Biological Science, University of Udine, P.le M. Kolbe 4, 33010, Udine, Italy

    Alfonso Colombatti

  6. Urology Department, Policlinico San Giorgio, Via Agostino Gemelli 10, 33170, Pordenone, PN, Italy

    Pietro Belmonte & Renzo Zucconelli

Authors
  1. Greta Del Mistro
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  2. Silvia Cervo
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  3. Elena Mansutti
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  4. Riccardo Spizzo
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  5. Alfonso Colombatti
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  6. Pietro Belmonte
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  7. Renzo Zucconelli
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  8. Agostino Steffan
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  9. Valter Sergo
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  10. Alois Bonifacio
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Corresponding author

Correspondence to Alois Bonifacio.

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Del Mistro, G., Cervo, S., Mansutti, E. et al. Surface-enhanced Raman spectroscopy of urine for prostate cancer detection: a preliminary study. Anal Bioanal Chem 407, 3271–3275 (2015). https://doi.org/10.1007/s00216-015-8610-9

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  • DOI: https://doi.org/10.1007/s00216-015-8610-9

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