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Hydrophilic interaction chromatography based solid-phase extraction and MALDI TOF mass spectrometry for revealing the influence of Pseudomonas fluorescens on phospholipids in salmon fillet

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Abstract

Salmon is a popular food but it is easily susceptible to spoilage by contamination with microorganisms. In this study, a method using hydrophilic interaction chromatography (HILIC)-based solid-phase extraction (SPE) and matrix-assisted laser desorption and ionization time-of-flight/time-of-flight mass spectrometry was developed and applied to reveal the effect of Pseudomonas fluorescens on salmon fillet during the shelf-life period by measuring the changes in the levels of phosphatidylcholine and phosphatidylethanolamine. Fresh samples were inoculated with P. fluorescens (106 cfu g-1) for 30 s, and lipids were extracted at 0, 24, 48, and 72 h. A homemade SPE cartridge packed with HILIC sorbent (silica derivatized with 1,2-dihydroxypropane) was used for matrix cleanup prior to analysis by mass spectrometry. In total, 30 phospholipids and 16 lysophospholipids were detected and elucidated. The results revealed that the content of phospholipids decreased significantly, whereas that of lysophospholipids increased initially, followed by a gradual reduction as the cold storage time increased. The contamination by P. fluorescens negatively affected the quality of fresh salmon without obvious physical changes, but it posed a potential threat to human health. This study suggests that the well-established method could be used for detecting phospholipids in salmon fillet and perhaps other foods as well.

Freshness of salmon on sale in supermarket could be monitored by means of MALDI-TOF/MS

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Acknowledgments

Q. Shen thanks the SGS committee of City University of Hong Kong for granting a Ph.D. studentship. All the authors are thankful for the partial financial support from the HKCMMS project (9211051) for the purchase of consumable materials.

Conflict of interest

The authors declare they have no competing financial interest.

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Authors and Affiliations

  1. Department of Biomedical Sciences, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong Special Administrative Region, China

    Qing Shen, Qi Yang & Hon-Yeung Cheung

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  1. Qing Shen
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  2. Qi Yang
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  3. Hon-Yeung Cheung
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Correspondence to Hon-Yeung Cheung.

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Shen, Q., Yang, Q. & Cheung, HY. Hydrophilic interaction chromatography based solid-phase extraction and MALDI TOF mass spectrometry for revealing the influence of Pseudomonas fluorescens on phospholipids in salmon fillet. Anal Bioanal Chem 407, 1475–1484 (2015). https://doi.org/10.1007/s00216-014-8365-8

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  • DOI: https://doi.org/10.1007/s00216-014-8365-8

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