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A spiked tissue-based approach for quantification of phosphatidylcholines in brain section by MALDI mass spectrometry imaging

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Abstract

In the last few years, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has been successfully used to study the distribution of lipids within tissue sections. However, few efforts have been made to acquire reliable quantitative data regarding the localized concentrations of these molecules. Here we propose an approach based on brain homogenates for the quantification of phosphatidylcholines (PCs) in brain section by MALDI MSI. Homogenates were spiked with a range of PC(16:0 d31/18:1) concentrations. Sections from homogenates and intact brain were simultaneously prepared before being analyzed by MALDI MSI using a Fourier transform ion cyclotron resonance (FT-ICR) analyzer. Standard curves were generated from the signal intensity of the different PC(16:0 d31/18:1) ionic species ([M+H]+, [M+Na]+ and [M+K]+) detected from the homogenate sections. Localized quantitative data were finally extracted by correlating the standard curves with the signal intensities of endogenous PC (especially PC(16:0/18:1)) ionic species detected on different areas of the brain section. They were consistent with quantitative values found in the literature. This work introduces a new method to take directly into account biological matrix effects for the quantification of lipids as well as other endogenous compounds, in tissue sections by MALDI MSI.

A spiked tissue-based method for lipids quantification by MALDI mass spectrometry imaging.

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Abbreviations

a.u.:

Arbitrary unit

H&E:

Haematoxylin and eosin

ITO:

Indium tin oxide

MALDI:

Matrix-assisted laser desorption/ionization

MSI:

Mass spectrometry imaging

PC:

Phosphatidylcholine

R 2 :

Coefficient of determination

ROI:

Region of interest

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Acknowledgments

L. J. thanks the REFRACT project for funding (“Action de Recherche Concertée,” ULg, Belgium). R. L. is a postdoc fellow of the ULg Research Council. The FTMS instrument was acquired with European Funds for Regional Development (FEDER) and the FNRS.

Conflict of interest

The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Mass Spectrometry Laboratory, Department of Chemistry, GIGA-Research, University of Liège, 4000, Liège, Belgium

    Laure Jadoul, Rémi Longuespée & Edwin De Pauw

  2. Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liège, 4000, Liège, Belgium

    Agnès Noël

Authors
  1. Laure Jadoul
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  2. Rémi Longuespée
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  3. Agnès Noël
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  4. Edwin De Pauw
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Correspondence to Laure Jadoul or Edwin De Pauw.

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Published in the topical collection Mass Spectrometry Imaging with guest editors Andreas Römpp and Uwe Karst.

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Jadoul, L., Longuespée, R., Noël, A. et al. A spiked tissue-based approach for quantification of phosphatidylcholines in brain section by MALDI mass spectrometry imaging. Anal Bioanal Chem 407, 2095–2106 (2015). https://doi.org/10.1007/s00216-014-8232-7

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  • DOI: https://doi.org/10.1007/s00216-014-8232-7

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