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Fluorescence imaging of macromolecule transport in high molecular weight cut-off microdialysis

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Abstract

When microdialysis (MD) membrane exceeds molecular weight cut-off (MWCO) of 100 kDa, the fluid mechanics are in the ultrafiltration regime. Consequently, fluidic mass transport of macromolecules in the perfusate over the membrane may reduce the biological relevance of the sampling and cause an inflammatory response in the test subject. Therefore, a method to investigate the molecular transport of high MWCO MD is presented. An in vitro test chamber was fabricated to facilitate the fluorescent imaging of the MD sampling process, using fluoresceinylisothiocyanate (FITC) dextran and fluorescence microscopy. Qualitative studies on dextran behavior inside and outside the membrane were performed. Semiquantitative results showed clear dextran leakage from both 40 and 250 kDa dextran when 100 kDa MWCO membranes were used. Dextran 40 kDa leaked out with an order of magnitude higher concentration and the leakage pattern resembled more of a convective flow pattern compared with dextran 250 kDa, where the leakage pattern was more diffusion based. No leakage was observed when dextran 500 kDa was used as a colloid osmotic agent. The results in this study suggest that fluorescence imaging could be used as a method for qualitative and semiquantitative molecular transport and fluid dynamics studies of MD membranes and other hollow fiber catheter membranes.

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Acknowledgments

This research was in part supported by the Uppsala Berzelii Technology Centre for Neurodiagnostics, funded by the Swedish Governmental Agency for Innovation Systems and the Swedish Research Council Grant number P29797-1. The authors acknowledge mDialysis and Pharmacosomos AS for sharing their knowledge and for material support and Visualize your Science for graphical assistance.

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Authors and Affiliations

  1. Department of Engineering Sciences, Uppsala University, Box 534, 751 21, Uppsala, Sweden

    Jiangtao Chu, Vitali Koudriavtsev, Klas Hjort & Andreas P. Dahlin

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  1. Jiangtao Chu
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  2. Vitali Koudriavtsev
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  3. Klas Hjort
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  4. Andreas P. Dahlin
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Correspondence to Andreas P. Dahlin.

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Chu, J., Koudriavtsev, V., Hjort, K. et al. Fluorescence imaging of macromolecule transport in high molecular weight cut-off microdialysis. Anal Bioanal Chem 406, 7601–7609 (2014). https://doi.org/10.1007/s00216-014-8192-y

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  • DOI: https://doi.org/10.1007/s00216-014-8192-y

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