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LC-QTOF/MS metabolomic profiles in human plasma after a 5-week high dietary fiber intake

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Abstract

The objective was to investigate the alterations of plasma metabolome profiles to identify exposure and effect markers of dietary fiber intake. Subjects (n = 25) aged 58.6 (1.1) years (mean and SD) with a body mass index of 26.6 (0.5) kg/m2 were given a high fiber (HF) and a low fiber (LF) diet, in a 5-week randomized controlled crossover intervention. The HF diet consisted of oat bran, rye bran, and sugar beet fiber incorporated into test food products, whereas the LF diet was made of equivalent food products to the HF diet, but without adding fibers. Blood plasma samples were collected at the start and end of each intervention period and analyzed by LC-QTOF/MS. In total, 6 features in positive mode and 14 features in negative mode were significantly different between the HF and the LF diet (p < 0.01, q < 0.05). Two markers, 2,6-dihydroxybenzoic acid and 2-aminophenol sulfate, were increased after HF diet, along with a tentatively identified saponin derived from oat avenacosides. The untargeted metabolomics approach enabled the identification of two new markers of dietary fiber intake in human plasma. Further studies will be needed to verify if these markers could serve as compliance markers of fiber intake.

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Acknowledgments

We gratefully thank RN Ingrid Palmquist for performing the blood sampling, M.Sc. Daniela Rago for LC-QTOF/MS analysis of some of the samples, and participating companies (Findus, Lantmännen, Nordic Sugar and Oatly) for providing the dietary fibers and for the development and production of the food products used in this study. This work has been supported by Nordic Centre of Excellence SYSDIET (Systems biology in controlled dietary interventions and cohort studies, no. 070014), OPUS (Optimal well-being, development and health for Danish children through a healthy New Nordic Diet, supported by a grant from the Nordea Foundation), the European Network of Excellence NuGO (The European Nutrigenomics Organization), the EU Network of Excellence ECNIS2 (Environmental Cancer Risk , Nutrition and Individual Susceptibility) and a VINNOVA grant (project number 2004–02285). Biomedical Nutrition, Lund University and the Department of Nutrition, Exercise and Sports, University of Copenhagen are members of the NuGO Association.

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The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Biomedical Nutrition, Pure and Applied Biochemistry, Center for Applied Life Sciences, Department of Chemistry, Lund University, P.O. Box 124, 22100, Lund, Sweden

    Anna Johansson-Persson, Matilda Ulmius & Gunilla Önning

  2. Department of Nutrition, Exercise and Sports, University of Copenhagen, Rolighedsvej 30, 1958, Frederiksberg C, Denmark

    Thaer Barri & Lars Ove Dragsted

Authors
  1. Anna Johansson-Persson
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  2. Thaer Barri
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  3. Matilda Ulmius
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  4. Gunilla Önning
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  5. Lars Ove Dragsted
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Correspondence to Gunilla Önning.

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Johansson-Persson, A., Barri, T., Ulmius, M. et al. LC-QTOF/MS metabolomic profiles in human plasma after a 5-week high dietary fiber intake. Anal Bioanal Chem 405, 4799–4809 (2013). https://doi.org/10.1007/s00216-013-6874-5

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