Abstract
A selective aptameric sequence is adsorbed on a two-dimensional nanostructured metallic platform optimized for surface-enhanced Raman spectroscopy (SERS) measurements. Using nanofabrication methods, a metallic nanostructure was prepared by electron-beam lithography onto a glass coverslip surface and embedded within a microfluidic channel made of polydimethylsiloxane, allowing one to monitor in situ SERS fingerprint spectra from the adsorbed molecules on the metallic nanostructures. The gold structure was designed so that its localized surface plasmon resonance matches the excitation wavelength used for the Raman measurement. This optofluidic device is then used to detect the presence of a toxin, namely ochratoxin-A (OTA), in a confined environment, using very small amounts of chemicals, and short data acquisition times, by taking advantage of the optical properties of a SERS platform to magnify the Raman signals of the aptameric monolayer system and avoiding chemical labeling of the aptamer or the OTA target.
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References
Yamamoto R, Katahira M, Nishihira A, Baba T, Taira K, Kumar PKR (2000) Genes Cells 5:371–388
Fukuda K, Vishnuvardhan D, Sekiya S, Hwang J, Kakiuchi N, Kazunari T, Shimotohno K, Kumar PKR, Nishikawa S (2000) Eur J Biochem 267:3685–3694
Bock LC, Griffin LC, Latham JA, Vermaas EH, Toole JJ (1992) Nature 355:564–566
Torres-Chavolla E, Alocilja EC (2009) Biosens Bioelectron 24:3175–3182
Baker BR, Lai RY, Wood MS, Doctor EH, Heeger AJ, Plaxco KW (2006) J Am Chem Soc 128:3138–3139
Geiger A, Burgstaller P, von der Eltz H, Roeder A, Famulok M (1996) Nucleic Acids Res 24:1029–1036
Ueyama H, Takagi M, Takenaka S (2002) J Am Chem Soc 124:14286–14287
Zhang J, Wang L, Pan D, Song S, Boey FYC, Zhang H, Fan C (2008) Small 4:1196–1200
Ellington AD, Szostak JW (1990) Nature 346:818–822
Tuerk C, Gold L (1990) Science 249:505–510
Barthelmebs L, Jonca J, Hayat A, Prieto-Simon B, Marty J-L (2010) Food Control 22:737–743
Cruz-Aguado JA, Penner G (2008) J Agric Food Chem 56:10456–10461
Cruz-Aguado JA, Penner G (2008) Anal Chem 80:8853–8855
De Girolamo A, McKeague M, Miller JD, DeRosa MC, Visconti A (2011) Food Chem 127:1378–1384
Merino EJ, Weeks KM (2005) J Am Chem Soc 127:12766–12767
Nutiu R, Li Y (2005) Angew Chem Int Ed 44:5464–5467
Perrier S, Ravelet C, Guieu V, Fize J, Roy B, Perigaud C, Peyrin E (2010) Biosens Bioelec 25:1652–1657
Rhouati A, Paniel N, Meraihi Z, Marty J-L (2011) Food Control 22:1790–1796
Ruta J, Perrier S, Ravelet C, Fize J, Peyrin E (2009) Anal Chem 81:7468–7473
Sheng L, Ren J, Miao Y, Wang J, Wang E (2011) Biosens Bioelectron 26:3494–3499
Shlyahovsky B, Li D, Weizmann Y, Nowarski R, Kotler M, Willner I (2007) J Am Chem Soc 129:3814–3815
Tang Z, Mallikaratchy P, Yang R, Kim Y, Zhu Z, Wang H, Tan W (2008) J Am Chem Soc 130:11268–11269
Wang L, Chen W, Ma W, Liu L, Ma W, Zhao Y, Zhu Y, Xu L, Kuang H, Xu C (2011) Chem Commun 47:1574–1576
Zhu Z, Ravelet C, Perrier S, Guieu V, Roy B, Perigaud C, Peyrin E (2010) Anal Chem 82:4613–4620
Kidd A, Guieu V, Perrier S, Ravelet C, Peyrin E (2011) Anal Bioanal Chem 401(10):3229–3234
Zhu Z, Schmidt T, Mahrous M, Guieu V, Perrier S, Ravelet C, Peyrin E (2011) Anal Chim Acta 707(1–2):191–196
Bonel L, Vidal JC, Duato P, Castillo JR (2011) Biosens Bioelectron 26:3254–3259
Du Y, Li B, Wang F, Dong S (2009) Biosens Bioelectron 24:1979–1983
Ikebukuro K, Kiyohara C, Sode K (2004) Anal Lett 37:2901–2909
Sassolas A, Blum LJ, Leca-Bouvier BD (2009) Electroanalysis 21:1237–1250
Wang Z, Duan N, Hun X, Wu S (2010) Anal Bioanal Chem 398:2125–2132
Zuo X, Xiao Y, Plaxco KW (2009) J Am Chem Soc 131:6944–6945
Liu Y, Tuleouva N, Ramanculov E, Revzin A (2010) Anal Chem 82(19):8131–8136
Wanga G, Wanga Y, Chena L, Choo J (2010) Biosens Bioelectron 25:1859–1868
Yang C, Wang Y, Marty J-L, Yang X (2011) Biosens Bioelec 26:2724–2727
Guieu V, Ravelet C, Perrier S, Zhu Z, Cayez S, Peyrin E (2011) Anal Chim Acta 706(2):349–353
Breuzard G, Millot J-M, Riou J-F, Manfait M (2003) Anal Chem 75:4305–4311
Chen J, Jiang J, Gao X, Liu G, Shen G, Yu R (2008) Chem Eur J 14:8374–8382
Kim NH, Lee SJ, Moskovits M (2010) Nano Lett 10:4181–4185
Neumann O, Zhang D, Tam F, Lal S, Wittung-Stafshede P, Halas NJ (2009) Anal Chem 81:10002–10006
Ochsenkühn MA, Campbell CJ (2010) Chem Commun 46:2799–2801
Pagba CV, Lanea SM, Wachsmann-Hogiu S (2010) J Raman Spectrosc 41:241–247
Wei C, Jia G, Yuan J, Feng Z, Li C (2006) Biochemistry 45:6681–6691
Huh YS, Erickson D (2010) Biosens Bioelectron 25:1240–1243
Rusciano G, De Luca AC, Pesce G, Sasso A, Oliviero G, Amato J, Borbone N, D'Errico S, Piccialli V, Piccialli G, Mayol L (2011) Anal Chem 17:6849–6855
Hernandez Hierro JM, Garcia-Villanova RJ, Rodriguez Torrero P, Toruno Fonseca IM (2008) J Agric Food Chem 56:751–756
Pfohl-Leszkowicz A, Manderville RA (2007) Mol Nutr Food Res 51:61–99
Serra Bonvehi J (2004) J Agric Food Chem 52:6347–6352
Kabak B (2009) Food Chem Toxicol 47:348–352
Medina A, Valle-Algarra FM, Gimeno-Adelantado JV, Mateo R, Mateo F, Jimenez M (2006) J Chromat A 1121:178–183
Noonim P, Mahakarnchanakul W, Nielsen KF, Frisvad JC, Samson RA (2008) Int J Food Microbiol 128:197–202
Zezza F, Longobardi F, Pascale M, Eremin SA, Visconti A (2009) Anal Bioanal Chem 395:1317–1323
Coronel MB, Sanchis V, Ramos AJ, Marin S (2009) Food Chem Toxicol 47:2847–2852
McKeague M, Bradley CR, Girolamo AD, Visconti A, Miller JD, DeRosa MC (2010) Int J Mol Sci 11:4864–4881
O'Brien E, Dietrich DR (2005) Crit Rev Toxicol 35:33–60
Li T, Jeon K-S, Suh YD, Kim MG (2011) Chem Commun 32:9098–9100
Vidal JC, Duato P, Bonel L, Castillo JR (2009) Anal Bioanal Chem 394:575–582
Galarreta BC, Norton PR, Lagugné-Labarthet F (2011) Langmuir 27(4):1494–1498
Haes AJ, Van Duyne RP (2002) J Am Chem Soc 124:10596–10604
Duffy DC, McDonald JC, Schueller OJA, Whitesides GM (1998) Anal Chem 70:4974–4984
Xu Y, Takai M, Konno T, Ishihara K (2007) Lab Chip 7:196–206
West J, Becker M, Tombrink S, Manz A (2008) Micro total analysis systems: latest achievements. Anal Chem 80(12):4403–4419
Mingxu Y, Yan C, Lu P, Da H, Bincheng Y, Bangce Y, Weihong T (2011) Chem Science 2:1003–1010
Galarreta BC, Harté E, Marquestaut N, Norton PR, Lagugné-Labarthet F (2010) Phys Chem Chem Phys 12:6810–6816
Patrito N, McLachlan JM, Faria SN, Chan J, Norton PR (2007) Lab Chip 7:1813–1818
Balamurugan S, Obubuafo A, Soper SA, McCarley RL, Spivak DA (2006) Langmuir 22:6446–6453
Lakowicz JR (2006) Fluorescence anisotropy. In: Lakowicz JR (ed) Principles of fluorescence spectroscopy, 3rd edn. Springer, New York, pp 353–381
Lafleur L, Rice J, Thomas GJ (1972) Biopolymers 11:2423–2437
Nishimura Y, Tsuboi M (1986) J Mol Struct 146:123–153
Prescott B, Steinmetz W, Thomas GJ (1984) Biopolymers 23:235–256
Rappoport D, Shim S, Aspuru-Guzik A (2011) Phys Chem Lett 2:1254–1260
Thomas GJ (1999) Annu Rev Biophys Biomol Struct 28:1–27
Bredenkamp MW, Dillen JLM, van Rooyen PH, Steyn PS (1989) J Chem Soc Perkin Trans II:1835–1839
Galvis-Sánchez AC, Barros AS, Delgadillo I (2008) Anal Chim Acta 617:59–63
Acknowledgments
The authors wish to gratefully acknowledge the Nanofabrication Facility at Western University for the fabrication of the patterned substrates and microfluidic device. This research was funded by the Natural Sciences and Engineering Research Council of Canada Discovery Grant and by the Canada Research Chairs program.
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Galarreta, B.C., Tabatabaei, M., Guieu, V. et al. Microfluidic channel with embedded SERS 2D platform for the aptamer detection of ochratoxin A. Anal Bioanal Chem 405, 1613–1621 (2013). https://doi.org/10.1007/s00216-012-6557-7
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DOI: https://doi.org/10.1007/s00216-012-6557-7