Abstract
Recently, clandestine drug lab operators have attempted to bypass controlled substances laws and regulations with “designer” compounds chemically and pharmacologically similar to controlled substances. For example, “bath salts” have erupted onto the scene as “legal highs” containing cathinone analogs that have produced severe side effects in users worldwide. These products have sparked concern among law enforcement agencies, and emergency bans have been placed on the sale of such items. Despite the increasing number of designer drugs available, there are few comprehensive screening techniques for their detection and quantification in biological specimens. The liquid chromatography triple quadrupole tandem mass spectrometry (LC-QQQ-MS/MS) method presented here encompasses over thirty important compounds within the phenethylamine, tryptamine, and piperazine designer drug classes. Analytes were determined by LC-QQQ-MS/MS in the multiple-reaction monitoring mode after mixed-mode solid-phase extraction. The bioanalytical method was fully validated according to recommended international guidelines. The assay was selective for all analytes with acceptable accuracy and precision. Limits of quantification were in the range of 1–10 ng/mL for each compound with limits of detection near 10 pg/mL. In order to evaluate its applicability in a forensic toxicological setting, the validated method was used to analyze post-mortem specimens from two cases that were suspected of containing designer drugs. The method was able to identify and quantify seven of these compounds at concentrations as low as 11 ng/mL. The method should have wide applicability for rapid screening of important new drugs of abuse at high sensitivity in both post- and ante-mortem forensic analysis.
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References
Henderson GL (1988) J Forensic Sci 33:569–575
Carroll FI, Lewin AH, Mascarella SW, Seltzmann HH, Reddy PA (2012) Ann N Y Acad Sci 1248:18–38
Hill SL, Thomas SHL (2011) Clin Toxicol 49:705–719
Coppola M, Mondola R (2012) Toxicol Lett 211:144–149
Wohlfarth A, Weinmann W (2010) Bioanalysis 2:965–979
McGrane O, Simmons J, Jacobson E, Skinner C (2011) J Clin Toxicol 1:2
Prosser JM, Nelson LS (2012) J Med Toxicol 8:33–42
Boland DM, Andollo W, Hime GW, Hearn WL (2005) J Anal Toxicol 29:394–397
Tanaka E, Kamata T, Katagi M, Tsuchihashi H, Honda K (2006) Forensic Sci Int 163:152–154
Elliott S, Smith C (2008) J Anal Toxicol 32:172–177
Kölliker S, Oehme M (2004) Anal Bioanal Chem 378:1294–1304
Apollonio LG, Pianca DJ, Whittall IR, Maher WA, Kyd JM (2006) J Chromatogr B 836:111–115
Concheiro M, de Castro A, Quintela O, Cruz A, López-Rivadulla M (2007) J Anal Toxicol 31:573–580
Wohlfarth A, Weinmann W, Dresen S (2010) Anal Bioanal Chem 396:2403–2414
Ammann D, McLaren JM, Gerostamoulos D, Beyer J (2012) J Anal Toxicol 36:381–389
Kerrigan S, Banuelos S, Perrella L, Hardy B (2011) J Anal Toxicol 35:459–469
Bell C, George C, Kicman AT, Traynor A (2011) Drug Testing Anal 3:496–504
Sørensen LK (2011) J Chromatogr B 879:727–736
Vorce SP, Sklerov JH (2004) J Anal Toxicol 28:407–410
Peters FT, Drummer OH, Musshoff F (2007) Forensic Sci Int 165:216–224
Matuszewski BK, Constanzer ML, Chavez-Eng CM (2003) Anal Chem 75:3019–3030
Araujo P (2009) J Chromatogr B 877:2224–2234
Peters FT, Remane D (2012) Anal Bioanal Chem 403:2155–2172
Wieling J, Hendriks G, Tamminga WJ, Hempenius J, Mensink CK, Oosterhuis B, Jonkman JHG (1996) J Chromatogr A 730:381–394
Cohen BMZ, Butler R (2011) Int J Drug Policy 22:95–101
Acknowledgments
The authors wish to thank Agilent Technologies for generously providing the LC-QQQ-MS instrumentation for this research.
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Swortwood, M.J., Boland, D.M. & DeCaprio, A.P. Determination of 32 cathinone derivatives and other designer drugs in serum by comprehensive LC-QQQ-MS/MS analysis. Anal Bioanal Chem 405, 1383–1397 (2013). https://doi.org/10.1007/s00216-012-6548-8
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DOI: https://doi.org/10.1007/s00216-012-6548-8