Analytical and Bioanalytical Chemistry

, Volume 403, Issue 6, pp 1491–1500

Recent advances in sulfotransferase enzyme activity assays

  • Priscilla Paul
  • Jiraporn Suwan
  • Jian Liu
  • Jonathan S. Dordick
  • Robert J. Linhardt
Review

DOI: 10.1007/s00216-012-5944-4

Cite this article as:
Paul, P., Suwan, J., Liu, J. et al. Anal Bioanal Chem (2012) 403: 1491. doi:10.1007/s00216-012-5944-4

Abstract

Sulfotransferases are enzymes that catalyze the transfer of sulfo groups from a donor, for example 3′-phosphoadenosine 5′-phosphosulfate, to an acceptor, for example the amino or hydroxyl groups of a small molecule, xenobiotic, carbohydrate, or peptide. These enzymes are important targets in the design of novel therapeutics for treatment of a variety of diseases. This review examines assays used for this important class of enzyme, paying particular attention to sulfotransferases acting on carbohydrates and peptides and the major challenges associated with their analysis.

Figure

When a sulfotransferase transfers a sulfo group from PAPS to RXH (X= or NH) substrate the reaction can be assayed using radiometric, photometric, fluorometric or mass spectrometric methods

Keywords

Sulfotransferases Sulfonation Carbohydrates Enzyme assays Peptides 

Abbreviations

ADP

Adenosine diphosphate

APS

Adenosine 5′-phosphosulfate

AST-IV

Aryl sulfotransferase type IV

ATP

Adenosine triphosphate

CDSNS

Completely de-sulfonated N-sulfonated heparin

DEAE

Diethylaminoethyl

EDC

Endocrine-disrupting compound

EMMA

Electrophoresis-mediated microanalysis

ESI–MS

Electrospray ionization–mass spectrometry

GlcNAc

N-Acetylglucosamine

hDHEA-ST

Human dehydroepiandrosterone sulfotransferase

HEC

High endothelial cell

HPLC

High-performance liquid chromatography

IP

Ion-pairing

kcat

Turnover number

Ki

Inhibition constant

Km

Michaelis–Menten constant

MU

Methylumbelliferone

NDST-1

N-Deacetylase N-sulfotransferase type 1

NodST

Bacterial carbohydrate sulfotransferase

N-ST

N-Sulfotransferase

NPAPS

3′-Phosphoadenosine 5′-phosphosulfate

O-ST

O-Sulfotransferase

PNP

p-Nitrophenyl

PNPS

p-Nitrophenylsulfate

PPi

Pyrophosphate

RP

Reversed phase

SDS-PAGE

Sodium dodecyl sulfate–polyacrylamide gel electrophoresis

ST

Sulfotransferase

STF0

Mycobacterial carbohydrate sulfotransferase

SULT-1A1

Phenyl sulfotransferase type 1A1

SULT-1A3

Phenyl sulfotransferase type 1A3

SULT1E1

Estrogen sulfotransferase type 1E1

TLC

Thin-layer chromatography

TM

Transmembrane

Vmax

Maximum velocity

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Priscilla Paul
    • 1
  • Jiraporn Suwan
    • 2
  • Jian Liu
    • 3
  • Jonathan S. Dordick
    • 1
    • 4
    • 5
  • Robert J. Linhardt
    • 1
    • 2
    • 4
    • 5
  1. 1.Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA
  2. 2.Department of Chemistry and Chemical BiologyRensselaer Polytechnic InstituteTroyUSA
  3. 3.Division of Medicinal Chemistry and National Products, College of PharmacyUniversity of North CarolinaChapel HillUSA
  4. 4.Department of BiologyRensselaer Polytechnic InstituteTroyUSA
  5. 5.Department of Biomedical EngineeringRensselaer Polytechnic InstituteTroyUSA