Analytical and Bioanalytical Chemistry

, Volume 402, Issue 6, pp 2217–2220

Highly sensitive detection of microRNA by chemiluminescence based on enzymatic polymerization

  • Changbei Ma
  • Edward S. Yeung
  • Shengda Qi
  • Rui Han
Technical Note

DOI: 10.1007/s00216-011-5653-4

Cite this article as:
Ma, C., Yeung, E.S., Qi, S. et al. Anal Bioanal Chem (2012) 402: 2217. doi:10.1007/s00216-011-5653-4

Abstract

We have developed a new methodology for miRNA assay using chemiluminescence imaging by poly(U) polymerase catalyzed miRNA polymerization. This method is very sensitive with a 50 fM limit of detection, which is comparable to or better than current assay methods. Multiplex detection for miRNA can be easily realized by introducing different capture probes onto the biosensor array, which will make it highly versatile for various research purposes.

Keywords

MicroRNAChemiluminescenceArray

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Changbei Ma
    • 1
  • Edward S. Yeung
    • 1
  • Shengda Qi
    • 1
  • Rui Han
    • 1
  1. 1.Ames Laboratory-USDOE and Department of ChemistryIowa State UniversityAmesUSA