Technical Note

Analytical and Bioanalytical Chemistry

, Volume 402, Issue 6, pp 2217-2220

Highly sensitive detection of microRNA by chemiluminescence based on enzymatic polymerization

  • Changbei MaAffiliated withAmes Laboratory-USDOE and Department of Chemistry, Iowa State University
  • , Edward S. YeungAffiliated withAmes Laboratory-USDOE and Department of Chemistry, Iowa State University Email author 
  • , Shengda QiAffiliated withAmes Laboratory-USDOE and Department of Chemistry, Iowa State University
  • , Rui HanAffiliated withAmes Laboratory-USDOE and Department of Chemistry, Iowa State University

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Abstract

We have developed a new methodology for miRNA assay using chemiluminescence imaging by poly(U) polymerase catalyzed miRNA polymerization. This method is very sensitive with a 50 fM limit of detection, which is comparable to or better than current assay methods. Multiplex detection for miRNA can be easily realized by introducing different capture probes onto the biosensor array, which will make it highly versatile for various research purposes.

Keywords

MicroRNA Chemiluminescence Array