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Comparison of CIM discs and CPG glass as solid supports for bioanalytical columns used in allergen detection

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Abstract

This work presents a comparison of convective interaction media (CIM) and controlled pore glass (CPG) as solid supports for immunoglobulin antibodies used in bioanalytical detection of allergens in foodstuffs. A flow-injection manifold with highly sensitive thermal lens spectrometric detection was used for this purpose. Using beta-lactoglobulin, a milk allergen, as a model analyte, CIM disc supports had a higher linear range (0.2–3.5 μg L−1), better reproducibility (intra-day RSD = 1%, inter-day RSD = 10%), lower consumption of reagents, and better immunocolumn stability (1 month, over 240 injections of substrate), while providing comparable LODs (0.1 μg L−1). Application of CIM discs as solid supports in immunocolumns for allergen detection enables fast and sensitive screening of allergens in foodstuffs with sample throughput of up to eight samples per hour.

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Abbreviations

BLG:

β-Lactoglobulin

CIM:

Convective interaction media

CPG:

Controlled pore glass

TLS:

Thermal lens spectrometry

FIA:

Flow injection analysis

ELISA:

Enzyme linked immunosorbent assay

References

  1. Krämer PM, Franke A, Zherdev AV, Yazynina EV, Dzantiev BB (2005) Talanta 65:324–330

    Article  Google Scholar 

  2. Blincko S, Rongsen S, Decun S, Howes I, Edwards R (2002) In: Edwards R (ed) Immunodiagnostics a practical approach (A Practical Approach). Oxford University Press, Oxford, pp 51–100

    Google Scholar 

  3. Gómez-Taylor Corominas B, Pferzschner J, Catalá Icardo M, Lahuerta Zamora L, Martínez Calatayud J (2005) J Pharm Biomed Anal 39:281–284

    Article  Google Scholar 

  4. Girelli AM, Mattei E (2005) J Chromatogr B 819:3–16

    Article  CAS  Google Scholar 

  5. Bjarnason B, Bousios N, Eremin S, Johansson G (1997) Anal Chim Acta 347:111–120

    Article  CAS  Google Scholar 

  6. Hagedorn J, Kasper C, Freitag R, Tennikova T (1999) J Biotechnol 69:1–7

    Article  CAS  Google Scholar 

  7. Girelli AM, Mattei E, Messina A (2007) Sens Actuators B 121:515–521

    Article  Google Scholar 

  8. Vodopivec M, Podgornik A, Berovič M, Štrancar A (2003) J Chromatogr B 795:105–113

    Article  CAS  Google Scholar 

  9. Štrancar A, Podgornik A, Barut M, Necina R (2002) Adv Biochem Eng Biotechnol 76:49–85

    Google Scholar 

  10. Phillips TM, Krum JM (1998) J Chromatogr B 715:55–63

    Article  CAS  Google Scholar 

  11. Järvinen K-M, Mäkinen-Kiljunen S, Suomalainen H (1999) J Pediatr 135:506–512

    Article  Google Scholar 

  12. Hohensinner V, Maier I, Pittner F (2007) J Biotechnol 130:385–388

    Article  CAS  Google Scholar 

  13. Negroni L, Bernard H, Clement G, Chatel JM, Brune P, Frobert Y, Wal JM, Grassi J (1998) J Immunol Meth 220:25–37

    Article  CAS  Google Scholar 

  14. Van Hengel A, Anklam E, Taylor SL, Hefle SL (2007) In: Pico Y (ed) Food toxicants analysis. Amsterdam, Elsevier

    Google Scholar 

  15. Levin ME, Motala C, Lopata AL (2005) Pediatrics 116:1223–1225

    Article  Google Scholar 

  16. Limbut W, Thavarungkul P, Kanatharana P, Asawatreratanakul P, Limsakul C, Wongkittisuksa B (2004) Biosens Bioelectron 19:813–821

    Article  CAS  Google Scholar 

  17. Puerta A, Diez-Masa JC, de Frutos M (2005) Anal Chim Acta 537:69–80

    Article  CAS  Google Scholar 

  18. Šikovec M, Novič M, Hudnik V, Franko M (1995) J Chromatogr A 706:121–126

    Article  Google Scholar 

  19. Podgornik A, Jančar J, Merhar M, Kozamernik S, Glover D, Čuček K, Barut M, Štrancar A (2004) J Biochem Biophys Meth 60:179–189

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This work was financially supported by the Slovenian Research Agency, through research program P1-0034 and a young researcher’s grant to Ana Čevdek. Financial support for equipment was obtained from the EU and the Municipality of Nova Gorica through structural funds contract C1536-08S330265. The authors are also grateful to BIA Separations, for their kind donation of diol CIM micro discs, and to Dr Aleš Podgornik for inspiring discussions.

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Correspondence to Mladen Franko.

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Čevdek, A., Franko, M. Comparison of CIM discs and CPG glass as solid supports for bioanalytical columns used in allergen detection. Anal Bioanal Chem 398, 555–562 (2010). https://doi.org/10.1007/s00216-010-3921-3

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  • DOI: https://doi.org/10.1007/s00216-010-3921-3

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