Original Paper

Analytical and Bioanalytical Chemistry

, Volume 398, Issue 1, pp 425-434

Determination of organic acids in Vaccinium berry standard reference materials

  • Melissa M. PhillipsAffiliated withAnalytical Chemistry Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology Email author 
  • , Ryan J. CaseAffiliated withAnalytical Chemistry Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology
  • , Catherine A. RimmerAffiliated withAnalytical Chemistry Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology
  • , Lane C. SanderAffiliated withAnalytical Chemistry Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology
  • , Katherine E. SharplessAffiliated withAnalytical Chemistry Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology
  • , Stephen A. WiseAffiliated withAnalytical Chemistry Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology
  • , James H. YenAffiliated withStatistical Engineering Division, Information Technology Laboratory, National Institute of Standards and Technology

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Abstract

Nine organic acids (citric acid, galacturonic acid, glycolic acid, isocitric acid, malic acid, oxalic acid, quinic acid, shikimic acid, and tartaric acid) and two anions (phosphate and sulfate) were determined in a suite of Vaccinium berry-containing dietary supplement standard reference materials (SRMs). Following solvent extraction, three independent methods were utilized in the quantification of these compounds. The first method involved reversed-phase liquid chromatography with ultraviolet absorbance detection at 210 nm and isotope dilution mass spectrometry. The second method utilized ion chromatography with conductivity detection. Finally, gas chromatography with isotope dilution mass spectrometry detection was used following derivatization with N-methyl-N-trifluoroacetamide (MSTFA). The combined data from these methods was used for the assignment of organic acid levels in the seven candidate SRMs.

Keywords

Organic acids Berry Liquid chromatography Gas chromatography Ion chromatography Mass spectrometry Isotope dilution