Analytical and Bioanalytical Chemistry

, Volume 398, Issue 1, pp 425–434

Determination of organic acids in Vaccinium berry standard reference materials

Authors

    • Analytical Chemistry Division, Chemical Science and Technology LaboratoryNational Institute of Standards and Technology
  • Ryan J. Case
    • Analytical Chemistry Division, Chemical Science and Technology LaboratoryNational Institute of Standards and Technology
  • Catherine A. Rimmer
    • Analytical Chemistry Division, Chemical Science and Technology LaboratoryNational Institute of Standards and Technology
  • Lane C. Sander
    • Analytical Chemistry Division, Chemical Science and Technology LaboratoryNational Institute of Standards and Technology
  • Katherine E. Sharpless
    • Analytical Chemistry Division, Chemical Science and Technology LaboratoryNational Institute of Standards and Technology
  • Stephen A. Wise
    • Analytical Chemistry Division, Chemical Science and Technology LaboratoryNational Institute of Standards and Technology
  • James H. Yen
    • Statistical Engineering Division, Information Technology LaboratoryNational Institute of Standards and Technology
Original Paper

DOI: 10.1007/s00216-010-3916-0

Cite this article as:
Phillips, M.M., Case, R.J., Rimmer, C.A. et al. Anal Bioanal Chem (2010) 398: 425. doi:10.1007/s00216-010-3916-0

Abstract

Nine organic acids (citric acid, galacturonic acid, glycolic acid, isocitric acid, malic acid, oxalic acid, quinic acid, shikimic acid, and tartaric acid) and two anions (phosphate and sulfate) were determined in a suite of Vaccinium berry-containing dietary supplement standard reference materials (SRMs). Following solvent extraction, three independent methods were utilized in the quantification of these compounds. The first method involved reversed-phase liquid chromatography with ultraviolet absorbance detection at 210 nm and isotope dilution mass spectrometry. The second method utilized ion chromatography with conductivity detection. Finally, gas chromatography with isotope dilution mass spectrometry detection was used following derivatization with N-methyl-N-trifluoroacetamide (MSTFA). The combined data from these methods was used for the assignment of organic acid levels in the seven candidate SRMs.

Keywords

Organic acids Berry Liquid chromatography Gas chromatography Ion chromatography Mass spectrometry Isotope dilution

Copyright information

© US Government 2010