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Celiac disease diagnosis and gluten-free food analytical control

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Abstract

Celiac disease (CD) is an autoimmune enteropathy, characterized by an inappropriate T-cell-mediated immune response to the ingestion of certain dietary cereal proteins in genetically susceptible individuals. This disorder presents environmental, genetic, and immunological components. CD presents a prevalence of up to 1% in populations of European ancestry, yet a high percentage of cases remain underdiagnosed. The diagnosis and treatment should be made early since untreated disease causes growth retardation and atypical symptoms, like infertility or neurological disorders. The diagnostic criteria for CD, which requires endoscopy with small bowel biopsy, have been changing over the last few decades, especially due to the advent of serological tests with higher sensitivity and specificity. The use of serological markers can be very useful to rule out clinical suspicious cases and also to help monitor the patients, after adherence to a gluten-free diet. Since the current treatment consists of a life-long gluten-free diet, which leads to significant clinical and histological improvement, the standardization of an assay to assess in an unequivocal way gluten in gluten-free foodstuff is of major importance.

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Abbreviations

AGA:

anti-gliadin antibodies

CD:

celiac disease

EIs:

electrochemical immunosensores

EMA:

endomysial antibodies

ELISA:

enzyme-linked immunosorbent assay

Ig:

immunoglobulin

ROC:

receiver operating characteristics

tTG:

tissue transglutaminase

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Acknowledgments

This work was supported by a PhD grant (SFRH/BD/46351/2008) attributed to Marta Maria Pereira da Silva Neves by Fundação para a Ciência e Tecnologia (FCT) and Fundo Social Europeu (FSE).

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Authors and Affiliations

  1. Faculdade de Farmácia da Universidade do Porto, Rua Aníbal Cunha 164, 4099-030, Porto, Portugal

    Marta Maria Pereira da Silva Neves & Alice Santos-Silva

  2. REQUIMTE, Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072, Porto, Portugal

    Marta Maria Pereira da Silva Neves, Hendrikus Petrus Antonius Nouws & Cristina Delerue-Matos

  3. Departamento de Química Física y Analítica, Universidad de Oviedo, C/. Julian Clavería, 8, 33006, Oviedo, Spain

    Marta Maria Pereira da Silva Neves, Maria Begoña González-Garcia & Agustín Costa-García

  4. Instituto de Biologia Molecular e Celular da Universidade do Porto, Rua do campo Alegre 823, 4150-180, Porto, Portugal

    Alice Santos-Silva

Authors
  1. Marta Maria Pereira da Silva Neves
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  2. Maria Begoña González-Garcia
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  3. Hendrikus Petrus Antonius Nouws
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  4. Cristina Delerue-Matos
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  5. Alice Santos-Silva
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  6. Agustín Costa-García
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Correspondence to Agustín Costa-García.

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da Silva Neves, M.M.P., González-Garcia, M.B., Nouws, H.P.A. et al. Celiac disease diagnosis and gluten-free food analytical control. Anal Bioanal Chem 397, 1743–1753 (2010). https://doi.org/10.1007/s00216-010-3753-1

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  • DOI: https://doi.org/10.1007/s00216-010-3753-1

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