Analytical and Bioanalytical Chemistry

, Volume 396, Issue 3, pp 1025–1035

Laser desorption/ionization mass spectrometry analysis of monolayer-protected gold nanoparticles

Authors

  • Bo Yan
    • Department of ChemistryUniversity of Massachusetts
  • Zheng-Jiang Zhu
    • Department of ChemistryUniversity of Massachusetts
  • Oscar R. Miranda
    • Department of ChemistryUniversity of Massachusetts
  • Apiwat Chompoosor
    • Department of ChemistryUniversity of Massachusetts
    • Department of ChemistryUniversity of Massachusetts
    • Department of ChemistryUniversity of Massachusetts
Original Paper

DOI: 10.1007/s00216-009-3250-6

Cite this article as:
Yan, B., Zhu, Z., Miranda, O.R. et al. Anal Bioanal Chem (2010) 396: 1025. doi:10.1007/s00216-009-3250-6

Abstract

Monolayer-protected gold nanoparticles (AuNPs) feature unique surface properties that enable numerous applications. Thus, there is a need for simple, rapid, and accurate methods to confirm the surface structures of these materials. Here, we describe how laser desorption/ionization mass spectrometry (LDI-MS) can be used to characterize AuNPs with neutral, positively, and negatively charged surface functional groups. LDI readily desorbs and ionizes the gold-bound ligands to produce both free thiols and disulfide ions in pure and complex samples. We also find that LDI-MS can provide a semi-quantitative measure of the ligand composition of mixed-monolayer AuNPs by monitoring mixed disulfide ions that are formed. Overall, the LDI-MS approach requires very little sample, provides an accurate measure of the surface ligands, and can be used to monitor AuNPs in complex mixtures.

https://static-content.springer.com/image/art%3A10.1007%2Fs00216-009-3250-6/MediaObjects/216_2009_3250_Figa_HTML.gif
Figure

LDI-MS analysis of ligand percentage on mixed monolayer AuNPs.

Keywords

Nanoparticles Laser desorption/ionization Mass spectrometry Surface ligands

Supplementary material

216_2009_3250_MOESM1_ESM.pdf (159 kb)
ESM 1 Electronic supplementary material (PDF 162 kb)

Copyright information

© Springer-Verlag 2009