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MicroRNA detection by microarray

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Abstract

MicroRNAs (miRNAs) are a class of small noncoding RNAs ∼22 nt in length that regulate gene expression and play fundamental roles in multiple biological processes, including cell differentiation, proliferation and apoptosis as well as disease processes. The study of miRNA has thus become a rapidly emerging field in life science. The detection of miRNA expression is a very important first step in miRNA exploration. Several methodologies, including cloning, northern blotting, real-time RT-PCR, microRNA arrays and ISH (in situ hybridization), have been developed and applied successfully in miRNA profiling. This review discusses the main existing microRNA detection technologies, while emphasizing microRNA arrays.

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Acknowledgements

This work was funded by the National Key Basic Research and Development Program of China (No. 2007CB935702)

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Authors and Affiliations

  1. State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, 200031, China

    Wei Li & Kangcheng Ruan

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  1. Wei Li
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  2. Kangcheng Ruan
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Correspondence to Kangcheng Ruan.

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Li, W., Ruan, K. MicroRNA detection by microarray. Anal Bioanal Chem 394, 1117–1124 (2009). https://doi.org/10.1007/s00216-008-2570-2

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