Analytical and Bioanalytical Chemistry

, Volume 394, Issue 2, pp 437–446

Investigating the translocation of λ-DNA molecules through PDMS nanopores

Original Paper

DOI: 10.1007/s00216-008-2529-3

Cite this article as:
Sen, Y. & Karnik, R. Anal Bioanal Chem (2009) 394: 437. doi:10.1007/s00216-008-2529-3
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Abstract

We investigate the translocation of λ-DNA molecules through resistive-pulse polydimethylsiloxane (PDMS) nanopore sensors. Single molecules of λ-DNA were detected as a transient current increase due to the effect of DNA charge on ionic current through the pore. DNA translocation was found to deviate from a Poisson process when the interval between translocations was comparable to the duration of translocation events, suggesting that translocation was impeded during the presence of another translocating molecule in the nanopore. Characterization of translocation at different voltage biases revealed that a critical voltage was necessary to drive DNA molecules through the nanopore. Above this critical voltage, frequency of translocation events was directly proportional to DNA concentration and voltage bias, suggesting that transport of DNA from the solution to the nanopore was the rate limiting step. These observations are consistent with experimental results on transport of DNA through nanopores and nanoslits and the theory of hydrodynamically driven polymer flow in pores.

Keywords

NanoporeDNAResistive-pulseBiosensingPDMSTranslocation

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of Mechanical EngineeringMassachusetts Institute of TechnologyCambridgeUSA