Original Paper

Analytical and Bioanalytical Chemistry

, Volume 393, Issue 2, pp 615-621

First online:

Frontal analysis microchip capillary electrophoresis to study the binding of ligands to receptors derivatized on magnetic beads

  • Xiaojun LiuAffiliated withDepartment of Chemistry and Biochemistry, California State University, Los Angeles
  • , Frank A. GomezAffiliated withDepartment of Chemistry and Biochemistry, California State University, Los Angeles Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


The model binding of the glycopeptide antibiotic teicoplanin (Teic) from Actinoplanes teichomyceticus, immobilized on magnetic microspheres, to d-Ala-d-Ala terminus peptides was assessed using microchip capillary electrophoresis (MCE) with continuous frontal analysis (FA). Teic is closely related to vancomycin (Van), historically, the drug of last resort used to treat many Gram-positive bacterial infections. Glycopeptide antibiotics inhibit bacterial growth by binding to the d-Ala-d-Ala terminus on the cell wall of Gram-positive bacteria via hydrogen bonds, thereby preventing the enzyme-mediated cross-linking of peptidoglycan and eventual cell death. In this work direct and competitive bead-based assays in a microfluidic chip are demonstrated. The binding constants obtained using the technique are comparable with values reported in the literature.


Frontal analysis Magnetic microbeads Glycopeptide antibiotic