Analytical and Bioanalytical Chemistry

, Volume 393, Issue 4, pp 1157–1163

Label-free detection of cancer biomarker candidates using surface plasmon resonance imaging

Authors

  • Jon Ladd
    • Department of Chemical EngineeringUniversity of Washington
  • Allen D. Taylor
    • Department of Chemical EngineeringUniversity of Washington
  • Marek Piliarik
    • Institute of Photonics and Electronics of the Academy of Sciences of the Czech Republic
  • Jiri Homola
    • Institute of Photonics and Electronics of the Academy of Sciences of the Czech Republic
    • Department of Chemical EngineeringUniversity of Washington
Original Paper

DOI: 10.1007/s00216-008-2448-3

Cite this article as:
Ladd, J., Taylor, A.D., Piliarik, M. et al. Anal Bioanal Chem (2009) 393: 1157. doi:10.1007/s00216-008-2448-3

Abstract

In this work, we present an antibody array for the detection of cancer biomarker candidates by a surface plasmon resonance (SPR) imaging sensor with polarization contrast. Responses from the SPR imaging sensor are shown to be similar to those from a conventional spectroscopy-based SPR sensor. Antibodies are spotted onto a self-assembled monolayer (SAM) composed of oligo(ethylene glycol) (OEG)-containing alkanethiol chains. Detection of two cancer biomarker candidates, activated leukocyte cell adhesion molecule/CD 166 (ALCAM) and transgelin-2 (TAGLN2), is demonstrated. Limits of detection for ALCAM and TAGLN2 are established at 6 ng/mL and 3 ng/mL, respectively, in buffer. No cross-reactivity is observed between immobilized antibodies and nonspecific antigen. Biomarker candidates are also detected in a 10% human serum solution.

Keywords

Surface plasmon resonance imaging Protein array Cancer biomarkers

Supplementary material

216_2008_2448_MOESM1_ESM.pdf (126 kb)
ESM 1 (PDF 126 KB).

Copyright information

© Springer-Verlag 2008