Original Paper

Analytical and Bioanalytical Chemistry

, Volume 393, Issue 4, pp 1157-1163

Label-free detection of cancer biomarker candidates using surface plasmon resonance imaging

  • Jon LaddAffiliated withDepartment of Chemical Engineering, University of Washington
  • , Allen D. TaylorAffiliated withDepartment of Chemical Engineering, University of Washington
  • , Marek PiliarikAffiliated withInstitute of Photonics and Electronics of the Academy of Sciences of the Czech Republic
  • , Jiri HomolaAffiliated withInstitute of Photonics and Electronics of the Academy of Sciences of the Czech Republic
  • , Shaoyi JiangAffiliated withDepartment of Chemical Engineering, University of Washington Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

In this work, we present an antibody array for the detection of cancer biomarker candidates by a surface plasmon resonance (SPR) imaging sensor with polarization contrast. Responses from the SPR imaging sensor are shown to be similar to those from a conventional spectroscopy-based SPR sensor. Antibodies are spotted onto a self-assembled monolayer (SAM) composed of oligo(ethylene glycol) (OEG)-containing alkanethiol chains. Detection of two cancer biomarker candidates, activated leukocyte cell adhesion molecule/CD 166 (ALCAM) and transgelin-2 (TAGLN2), is demonstrated. Limits of detection for ALCAM and TAGLN2 are established at 6 ng/mL and 3 ng/mL, respectively, in buffer. No cross-reactivity is observed between immobilized antibodies and nonspecific antigen. Biomarker candidates are also detected in a 10% human serum solution.

Keywords

Surface plasmon resonance imaging Protein array Cancer biomarkers