Analytical and Bioanalytical Chemistry

, Volume 392, Issue 3, pp 427–438

Development of saw palmetto (Serenoa repens) fruit and extract standard reference materials

  • Michele M. Schantz
  • Mary Bedner
  • Stephen E. Long
  • John L. Molloy
  • Karen E. Murphy
  • Barbara J. Porter
  • Karsten Putzbach
  • Catherine A. Rimmer
  • Lane C. Sander
  • Katherine E. Sharpless
  • Jeanice B. Thomas
  • Stephen A. Wise
  • Laura J. Wood
  • James H. Yen
  • Takashi Yarita
  • Agnes NguyenPho
  • Wendy R. Sorenson
  • Joseph M. Betz
Original Paper

DOI: 10.1007/s00216-008-2297-0

Cite this article as:
Schantz, M.M., Bedner, M., Long, S.E. et al. Anal Bioanal Chem (2008) 392: 427. doi:10.1007/s00216-008-2297-0

Abstract

As part of a collaboration with the National Institutes of Health’s Office of Dietary Supplements and the Food and Drug Administration’s Center for Drug Evaluation and Research, the National Institute of Standards and Technology has developed two standard reference materials (SRMs) representing different forms of saw palmetto (Serenoa repens), SRM 3250 Serenoa repens fruit and SRM 3251 Serenoa repens extract. Both of these SRMs have been characterized for their fatty acid and phytosterol content. The fatty acid concentration values are based on results from gas chromatography with flame ionization detection (GC-FID) and mass spectrometry (GC/MS) analysis while the sterol concentration values are based on results from GC-FID and liquid chromatography with mass spectrometry analysis. In addition, SRM 3250 has been characterized for lead content, and SRM 3251 has been characterized for the content of β-carotene and tocopherols. SRM 3250 (fruit) has certified concentration values for three phytosterols, 14 fatty acids as triglycerides, and lead along with reference concentration values for four fatty acids as triglycerides and 16 free fatty acids. SRM 3251 (extract) has certified concentration values for three phytosterols, 17 fatty acids as triglycerides, β-carotene, and γ-tocopherol along with reference concentration values for three fatty acids as triglycerides, 17 fatty acids as free fatty acids, β-carotene isomers, and δ-tocopherol and information values for two phytosterols. These SRMs will complement other reference materials currently available with concentrations for similar analytes and are part of a series of SRMs being developed for dietary supplements.

Keywords

Saw palmettoSerenoa repensFatty acidsPhytosterolsCertified reference materialStandard reference material

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Michele M. Schantz
    • 1
  • Mary Bedner
    • 1
  • Stephen E. Long
    • 1
  • John L. Molloy
    • 1
  • Karen E. Murphy
    • 1
  • Barbara J. Porter
    • 1
  • Karsten Putzbach
    • 1
  • Catherine A. Rimmer
    • 1
  • Lane C. Sander
    • 1
  • Katherine E. Sharpless
    • 1
  • Jeanice B. Thomas
    • 1
  • Stephen A. Wise
    • 1
  • Laura J. Wood
    • 1
  • James H. Yen
    • 2
  • Takashi Yarita
    • 1
    • 3
  • Agnes NguyenPho
    • 4
  • Wendy R. Sorenson
    • 5
  • Joseph M. Betz
    • 6
  1. 1.Analytical Chemistry DivisionNational Institute of Standards and TechnologyGaithersburgUSA
  2. 2.Statistical Engineering DivisionNational Institute of Standards and TechnologyGaithersburgUSA
  3. 3.National Metrology Institute of JapanNational Institute of Advanced Industrial Science and TechnologyTsukubaJapan
  4. 4.Center for Drug Evaluation and Research (CDER)Food and Drug AdministrationSilver SpringUSA
  5. 5.Covance LaboratoriesMadisonUSA
  6. 6.Office of Dietary SupplementsNational Institutes of HealthBethesdaUSA