Original Paper

Analytical and Bioanalytical Chemistry

, Volume 392, Issue 3, pp 427-438

Development of saw palmetto (Serenoa repens) fruit and extract standard reference materials

  • Michele M. SchantzAffiliated withAnalytical Chemistry Division, National Institute of Standards and Technology Email author 
  • , Mary BednerAffiliated withAnalytical Chemistry Division, National Institute of Standards and Technology
  • , Stephen E. LongAffiliated withAnalytical Chemistry Division, National Institute of Standards and Technology
  • , John L. MolloyAffiliated withAnalytical Chemistry Division, National Institute of Standards and Technology
  • , Karen E. MurphyAffiliated withAnalytical Chemistry Division, National Institute of Standards and Technology
  • , Barbara J. PorterAffiliated withAnalytical Chemistry Division, National Institute of Standards and Technology
  • , Karsten PutzbachAffiliated withAnalytical Chemistry Division, National Institute of Standards and Technology
  • , Catherine A. RimmerAffiliated withAnalytical Chemistry Division, National Institute of Standards and Technology
  • , Lane C. SanderAffiliated withAnalytical Chemistry Division, National Institute of Standards and Technology
    • , Katherine E. SharplessAffiliated withAnalytical Chemistry Division, National Institute of Standards and Technology
    • , Jeanice B. ThomasAffiliated withAnalytical Chemistry Division, National Institute of Standards and Technology
    • , Stephen A. WiseAffiliated withAnalytical Chemistry Division, National Institute of Standards and Technology
    • , Laura J. WoodAffiliated withAnalytical Chemistry Division, National Institute of Standards and Technology
    • , James H. YenAffiliated withStatistical Engineering Division, National Institute of Standards and Technology
    • , Takashi YaritaAffiliated withAnalytical Chemistry Division, National Institute of Standards and TechnologyNational Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology
    • , Agnes NguyenPhoAffiliated withCenter for Drug Evaluation and Research (CDER), Food and Drug Administration
    • , Wendy R. SorensonAffiliated withCovance Laboratories
    • , Joseph M. BetzAffiliated withOffice of Dietary Supplements, National Institutes of Health

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Abstract

As part of a collaboration with the National Institutes of Health’s Office of Dietary Supplements and the Food and Drug Administration’s Center for Drug Evaluation and Research, the National Institute of Standards and Technology has developed two standard reference materials (SRMs) representing different forms of saw palmetto (Serenoa repens), SRM 3250 Serenoa repens fruit and SRM 3251 Serenoa repens extract. Both of these SRMs have been characterized for their fatty acid and phytosterol content. The fatty acid concentration values are based on results from gas chromatography with flame ionization detection (GC-FID) and mass spectrometry (GC/MS) analysis while the sterol concentration values are based on results from GC-FID and liquid chromatography with mass spectrometry analysis. In addition, SRM 3250 has been characterized for lead content, and SRM 3251 has been characterized for the content of β-carotene and tocopherols. SRM 3250 (fruit) has certified concentration values for three phytosterols, 14 fatty acids as triglycerides, and lead along with reference concentration values for four fatty acids as triglycerides and 16 free fatty acids. SRM 3251 (extract) has certified concentration values for three phytosterols, 17 fatty acids as triglycerides, β-carotene, and γ-tocopherol along with reference concentration values for three fatty acids as triglycerides, 17 fatty acids as free fatty acids, β-carotene isomers, and δ-tocopherol and information values for two phytosterols. These SRMs will complement other reference materials currently available with concentrations for similar analytes and are part of a series of SRMs being developed for dietary supplements.

Keywords

Saw palmetto Serenoa repens Fatty acids Phytosterols Certified reference material Standard reference material