Skip to main content
SpringerLink
Log in

Anion exchange silica monolith for capillary liquid chromatography

  • Technical Note
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

An anion exchange monolithic silica capillary column was prepared by surface modification of a hybrid monolithic silica capillary column prepared from a mixture of tetramethoxysilane (TMOS) and methyltrimethoxysilane (MTMS). The surface modification was carried out by on-column copolymerization of N-[3-(dimethylamino)propyl]acrylamide methyl chloride-quaternary salt (DMAPAA-Q) with 3-methacryloxypropyl moieties bonded as an anchor to the silica surface to form a strong anion exchange stationary phase. The columns were examined for their performance in liquid chromatography (LC) and capillary electrochromatography (CEC) separations of common anions. The ions were separated using 50 mM phosphate buffer at pH 6.6. Evaluation by LC produced an average of 30,000 theoretical plates (33 cm column length) for the inorganic anions and nucleotides. Evaluation by CEC, using the same buffer, produced enhanced chromatographic performance of up to ca. 90,000 theoretical plates and a theoretical plate height of ca. 4 μm. Although reduced efficiency was observed for inorganic anions that were retained a long time, the results of this study highlight the potential utility of the DMAPAA-Q stationary phase for anion separations.

Micro-LC performance evaluation of a strong anion exchange silica monolith column, 100H-MOP-DMAPAA-Q, 33 cm in length, with a mobile phase of 50 mM phosphate buffer, pH 2.8; linear velocity: u = 1.8 mm/s; UV-Vis detection at 254 nm. Sample solution (5 mg/mL of each component, 4 mL) was injected in split flow injection mode at a split ratio of ca. 1:1900 with a pump flow rate of 1.5 mL/min

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Abbreviations

CEC:

Capillary electrochromatography

TMOS:

Tetramethoxysilane

MTMS:

Methyl trimethoxysilane

DMAPAA-Q:

N-[3-(dimethylamino)propyl]acrylamide methyl chloride-quaternary salt

MOP:

3-Methacryloxypropyl

References

  1. Paull B, Nesterenko PN (2005) TrAC 24:295–303

    CAS  Google Scholar 

  2. Chambers SD, Glenn KM, Lucy CA (2007) J Sep Sci 30:1628–1645

    Article  CAS  Google Scholar 

  3. Suzuki A, Lim LW, Hiroi T, Takeuchi T (2006) Talanta 70:190–193

    Article  CAS  Google Scholar 

  4. Zakaria P, Hutchinson JP, Avdalovic N, Liu Y, Haddad PR (2005) Anal Chem 77:417–423

    Article  CAS  Google Scholar 

  5. Riordain CO, Gillespie E, Connolly D, Nesterenko PN, Paull B (2007) J Chromatogr A 1142:185–193

    Article  CAS  Google Scholar 

  6. Glenn KM, Lucy CA, Haddad PR (2007) J Chromatogr A 1155:8–14

    Article  CAS  Google Scholar 

  7. Hatsis P, Lucy CA (2003) Anal Chem 75:995–1001

    Article  CAS  Google Scholar 

  8. Ikegami T, Ichimaru J, Kajiwara W, Nagasawa N, Hosoya K, Tanaka N (2007) Anal Sci 23:1–6

    Article  Google Scholar 

  9. Pelletier S, Lucy CA (2006) J Chromatogr A 1118:12–18

    Article  CAS  Google Scholar 

  10. Scherer B, Steiner F (2001) J Chromatogr A 924:197–209

    Article  CAS  Google Scholar 

  11. Hutchinson JP, Hilder EF, Macka M, Avdalovic N, Haddad PR (2006) J Chromatogr A 1109:10–18

    Article  CAS  Google Scholar 

  12. Lin TA, Li GY, Chau LK (2006) Anal Chim Acta 576:117–123

    Article  CAS  Google Scholar 

  13. Motokawa M, Kobayashi H, Ishizuka N, Minakuchi H, Nakanishi K, Jinnai H, Hosoya K, Ikegami T, Tanaka N (2002) J Chromatogr A 961:53–63

    Article  CAS  Google Scholar 

  14. Nunez O, Ikegami T, Kajiwara W, Miyamoto K, Horie K, Tanaka N (2007) J Chromatogr A 1156:35–44

    Article  CAS  Google Scholar 

  15. Breadmore MC, Shrinivasan S, Wolfe KA, Power ME, Ferrance JP, Hosticka B, Norris PM, Landers JP (2002) Electrophoresis 23:3487–3495

    Article  CAS  Google Scholar 

  16. Ishizuka N, Minakuchi H, Nakanishi K, Soga N, Nagayama H, Hosoya K, Tanaka N (2000) Anal Chem 72:1275–1280

    Article  CAS  Google Scholar 

  17. Horie K, Ikegami T, Hosoya K, Saad N, Fiehn O, Tanaka N (2007) J Chromatogr A 1164:198–205

    Article  CAS  Google Scholar 

Download references

Acknowledgement

JJ acknowledges the financial support from the JSPS-RONPAKU fellowship, VCC-10514, funded by the Japan Society for the Promotion of Science.

Author information

Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM, Skudai, Malaysia

    Jafariah Jaafar

  2. Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan

    Yuta Watanabe, Tohru Ikegami, Kosuke Miyamoto & Nobuo Tanaka

Authors
  1. Jafariah Jaafar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yuta Watanabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tohru Ikegami
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kosuke Miyamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nobuo Tanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jafariah Jaafar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jaafar, J., Watanabe, Y., Ikegami, T. et al. Anion exchange silica monolith for capillary liquid chromatography. Anal Bioanal Chem 391, 2551–2556 (2008). https://doi.org/10.1007/s00216-008-2063-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-008-2063-3

Keywords

Search

Navigation