Skip to main content
SpringerLink
Log in

Miniaturized monolithic disks for immunoadsorption of cardiac biomarkers from serum

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Immunoadsorbers based on 2.0 × 6.0 mm i.d., epoxy-bearing, methacrylate-based monolithic disks were developed in order to target myoglobin and N-terminal pro-natriuretic peptide (NT-proBNP), two biomarkers involved in cardiovascular disease. In both cases, antibodies were successfully coupled to the polymeric disk material. The developed immunoadsorbers permitted the selective isolation of myoglobin and NT-proBNP from human serum. Myoglobin was successfully isolated and detected from serum samples at concentrations down to 250 fmol μL−1. However, the affinity of the antibodies was not sufficient for the analysis of low-concentration clinical samples. Frontal analysis of anti-NT-proBNP disks revealed the ability of the immunoadsorber to bind up to 250 pmol NT-proBNP, which is more than sufficient for the analysis of clinical samples. Anti-NT-proBNP disks showed good stability over more than 18 months and excellent batch-to-batch reproducibility. Moreover, anti-NT-proBNP disks permitted the isolation of NT-proBNP at concentrations down to 750 amol μL−1 in serum, corresponding to concentrations of strongly diseased patients. Using reversed-phase trapping columns, the detection of NT-proBNP eluted from immunoadsorbers by mass spectrometry was achieved for concentrations down to 7.8 fmol μL−1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1a–b
Fig. 2
Fig. 3a–b
Fig. 4
Fig. 5
Fig. 6a–c
Fig. 7a–b

Similar content being viewed by others

References

  1. Marko-Varga G (2005) Proteomics and peptidomics: new technology platforms elucidating biology. Elsevier, Amsterdam

  2. Hu S, Loo JA, Wong DT (2006) Proteomics 6:6326–6353

    Article  CAS  Google Scholar 

  3. Zolg JW, Langen H (2004) Mol Cell Proteomics 3:345–354

    Google Scholar 

  4. De Lemos JA, McGuire DK, Drazner MH (2003) Lancet 362:316–322

    Article  CAS  Google Scholar 

  5. Richards AM, Nicholls MG, Yandle TG, Frampton C, Espiner EA, Turner JG, Buttimore RC, Lainchbury JG, Elliott JM, Ikram H, Crozier IG, Smyth DW (1998) Circulation 97:1921–1929

    CAS  Google Scholar 

  6. Kagen L, Scheidt S, Roberts L, Porter A, Paul H (1975) Am J Med 58:177–182

    Article  CAS  Google Scholar 

  7. Cloonan MJ, Bishop GA, Wilton-Smith PD, Carter IW, Allan RM, Wilcken DE (1979) Pathology 11:689–699

    CAS  Google Scholar 

  8. Beuerle JR, Azzazy HM, Styba G, Duh SH, Christenson RH (2000) Clin Chim Acta 294:115–128

    Article  CAS  Google Scholar 

  9. Bakker AJ, Koelemay MJ, Gorgels JP, van Vlies B, Smits R, Tijssen JG, Haagen FD (1994) Eur Heart J 15:45–53

    CAS  Google Scholar 

  10. Lavin F, Kane M, Forde A, Gannon F, Daly K (1995) Br Heart J 73:422–427

    Article  CAS  Google Scholar 

  11. Mayr BM, Kohlbacher O, Reinert K, Sturm M, Gropl C, Lange E, Klein C, Huber CG (2006) J Proteome Res 5:414–421

    Article  CAS  Google Scholar 

  12. Wu WW, Wang G, Baek SJ, Shen RF (2006) J Proteome Res 5:651–658

    Article  CAS  Google Scholar 

  13. Gerber SA, Rush J, Stemman O, Kirschner MW, Gygi SP (2003) Proc Natl Acad Sci USA 100:6940–6945

    Google Scholar 

  14. Zolg W (2006) Mol Cell Proteomics 5:1720–1726

    Article  CAS  Google Scholar 

  15. Karl J, Borgya A, Gallusser A, Huber E, Krueger K, Rollinger W, Schenk J (1999) Scand J Clin Lab Invest Suppl 230:177–181

    Article  CAS  Google Scholar 

  16. Panteghini M, Linsinger T, Wu AHB, Dati F, Apple FS, Christenson RH, Mair J, Schimmel H (2004) Clin Chim Acta 341:65–72

    Article  CAS  Google Scholar 

  17. Chien TI, Chen HH, Kao JT (2006) Clin Chim Acta 369:95–99

    Article  CAS  Google Scholar 

  18. Larsson PO, Glad M, Hansson L, Mansson MO, Ohlson S, Mosbach K (1983) Adv Chromatogr 21:41–84

    CAS  Google Scholar 

  19. Svec F, Huber CG (2006) Anal Chem 78:100–107

    Article  Google Scholar 

  20. Lim YP, Josic D, Callanan H, Brown J, Hixson DC (2005) J Chromatogr A 1065:39–43

    Article  CAS  Google Scholar 

  21. Rucevic M, Clifton JG, Huang F, Li X, Callanan H, Hixson DC, Josic D (2006) J Chromatogr A 1123:199–204

    Article  CAS  Google Scholar 

  22. Kasper C, Meringova L, Freitag R, Tennikova T (1998) J Chromatogr A 798:65–72

    Article  CAS  Google Scholar 

  23. Pan Z, Zou H, Mo W, Huang X, Wu R (2002) Anal Chim Acta 466:141–150

    Article  CAS  Google Scholar 

  24. Strancar A, Barut M, Podgornik A, Koselj P, Josic D, Buchacher A (1998) LC GC 11:660–670

    Google Scholar 

  25. Branovic K, Lattner G, Barut M, Strancar A, Josic Dj, Buchacher A (2002) J Immunol Methods 271:47–58

    Article  CAS  Google Scholar 

  26. Weber SG (1989) Talanta 36:99

    Article  CAS  Google Scholar 

  27. Gupalova TV, Lojkina OV, Palagnuk VG, Totolian AA, Tennikova TB (2002) J Chromatogr A 949:185–193

    Article  CAS  Google Scholar 

  28. Blackburn GF, Shah HP, Kenten JH, Leland J, Kamin RA, Link J, Peterman J, Powell MJ, Shah A, Talley DB, Tyagi SK, Wilkins E, Wu T-G, Massey RJ (1991) Clin Chem 37:1534–1539

    CAS  Google Scholar 

  29. Premstaller A, Oberacher H, Huber CG (2000) Anal Chem 72:4386–4393

    Article  CAS  Google Scholar 

  30. Schley C, Swart R, Huber CG (2006) J Chromatogr A 1136:210–220

    Article  CAS  Google Scholar 

  31. Hage DS (2002) J Chromatogr B 768:3–30

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, Instrumental Analysis and Bioanalysis, Saarland University, P.O. Box 151150, 66041, Saarbrücken, Germany

    N. Delmotte & C. G. Huber

  2. Roche Diagnostics GmbH, Nonnenwald 2, 82377, Penzberg, Germany

    U. Kobold, T. Meier & A. Gallusser

  3. Bia Separations, Ljubljana, Slovenia

    A. Strancar

Authors
  1. N. Delmotte
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. U. Kobold
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. Meier
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Gallusser
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Strancar
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. C. G. Huber
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to U. Kobold or C. G. Huber.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Delmotte, N., Kobold, U., Meier, T. et al. Miniaturized monolithic disks for immunoadsorption of cardiac biomarkers from serum. Anal Bioanal Chem 389, 1065–1074 (2007). https://doi.org/10.1007/s00216-007-1515-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-007-1515-5

Keywords

Search

Navigation