Abstract
A simple, fast method is described for the determination of Ag, As, Cd, Cu, Cr, Fe, Ni, and Se in marine biological tissues by electrothermal atomic-absorption spectrometry (ETAAS) and Na, Ca, K, Mg, Fe, Cu, and Mn by inductively coupled plasma–atomic emission spectrometry (ICP–AES). Solubilization of the biological tissue was achieved by using formic acid with vortex mixing followed by heating to 50°C in an ultrasonic bath. Once solubilized, the tissues were diluted to an appropriate volume with water for analysis. Aliquots were sampled into a graphite furnace and ICP–AES using a conventional autosampler. The method was validated by use of biological certified reference materials from NRC, DORM-2, DOLT-2, DOLT-3, LUTS-1, TORT-2, and NIST SRMs 1566b and 2976. Simplicity and reduced sample-preparation time prove to be the major advantages to the technique.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Subramanian K (1996) Spectrochim Acta Part B 51:291–319
Kingston HM, Jassie LB (eds) (1988) Introduction to microwave sample preparation: theory and practice ACS professional reference book series. American Chemical Society, Washington DC
Moeller A, Ambrose RF, Que Hee SS (2001) Food Addit Contam 18:19–29
Novozamsky I, Van der Lee HJ, Houba VJG (1995) Microchim Acta 119:183–189
Brisbin JAC, Caruso JA (2002) Analyst 127:921–929
Ashley K, Andrews RN, Cavazos L, Demange M (2001) J Anal Atom Spectrom 16:1147–1153
Balarama Krishna MV, Arunachalam J (2004) Anal Chim Acta 522:179–187
Peña-Farfal C, Moreda-Piñeiro A, Bermejo-Barrera A, Bermejo-Barrera P, Pinochet-Cancino H, de Gregori-Henríquez I (2004) Anal Chem 76:3541–3547
Wanekaya AK, Myung S, Sadik OA (2002) Analyst 127:1272–1276
Ebdon L, Fisher AS, Betti M, Leroy M (2003) In: Mester Z, Sturgeon RE (eds) Sample preparation for trace element analysis, Chap. 5. Elsevier BV, Amsterdam, pp 117–192
Tan Y, Blais JS, Marshall WD (1996) Analyst 121:483–488
Miller-Ihli NJ (1989) J Anal Atom Spectrom 4:295–297
Bendicho C, Margaretha TC, de Loos-Vollebregt MTC (1990) Spectrochim Acta Part B 45:679–693
Silva RGL, Willie SN, Sturgeon RE, Santelli RE, Sella SM (1999) Analyst 124:1843–1846
Tao G, Willie SN, Sturgeon RE (1998) Analyst 123:1215–1218
Martins P, Pozebon D, Dressler VL, Kemieciki GA (2002) Anal Chim Acta 470:195–204
Giacomell MBO, Lima MC, Stupp V, de Carvalho Junior RM, da Silva JBB, Bermejo Barrera P (2002) Spectrochim Acta B 57:2151–2157
Pozebon D, Dressler VL, Curtius AJ (1998) J Anal Atom Spectrom 13:1101–1105
Zhou CY, Wong MK, Koh LL, Wee YC (1996) Talanta 43:1061–1068
Perkin–Elmer (1991) Publication B3210: The THGA graphite furnace: techniques and recommended conditions. Perkin–Elmer, Norwalk CT USA
Liberski PP, Yanagihara R, Brown P, Kordek R, Kloszewska I, Bratosiewicz J, Gajdusek DC (1996) Neurodegeneration 5:95–99
Tsalev DL, Slaveykova VI (1998) Chemical modification in electrothermal atomic absorption spectrometry. In: Sneddon J (ed.) Advances in atomic spectroscopy IV. JAI Press, Greenwich, pp. 27–150
Slavin W (1984) Graphite furnace AAS: a source book. Perkin–Elmer Corporation, Norwalk CT USA
Wahlen R, McSheehy S, Scriver C, Mester Z (2004) J Anal Atom Spectrom 19:876–882
Deaker M, Maher W (1999) J Anal Atom Spectrom 14:1193–1207
CRM DORM-2 Certificate (2003) National Research Council of Canada. Ottawa ON, Canada
Ribeiro AS, Curtius AJ, Pozebon D (2000) Microchem J 64:105–110
Almeida AA, Lima JLFC (2000) J Anal Atom Spectrom 15:1019–1024
Acknowledgments
The authors thank P. Grinberg for the ICP–MS determinations.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Scriver, C., Kan, M., Willie, S. et al. Formic acid solubilization of marine biological tissues for multi-element determination by ETAAS and ICP–AES. Anal Bioanal Chem 381, 1460–1466 (2005). https://doi.org/10.1007/s00216-005-3125-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-005-3125-4