Reinstatement of ethanol and sucrose seeking by the neurosteroid allopregnanolone in C57BL/6 mice
- Deborah A. FinnAffiliated withVAMC Research (R&D-49), Department of Veterans Affairs Medical CenterDepartment of Behavioral Neuroscience, Oregon Health & Science University Email author
- , Gregory P. MarkAffiliated withDepartment of Behavioral Neuroscience, Oregon Health & Science University
- , Andrea M. FretwellAffiliated withVAMC Research (R&D-49), Department of Veterans Affairs Medical Center
- , Katherine R. Gililland-KaufmanAffiliated withDepartment of Behavioral Neuroscience, Oregon Health & Science University
- , Moriah N. StrongAffiliated withDepartment of Behavioral Neuroscience, Oregon Health & Science University
- , Matthew M. FordAffiliated withDepartment of Behavioral Neuroscience, Oregon Health & Science University
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Recent work in our laboratory documented that the “sipper” method of operant ethanol self-administration produced high ethanol intake and blood ethanol concentrations as well as the typical extinction “burst” in responding under nonreinforced conditions in male C57BL/6 mice. However, the neurochemical basis for reinstatement of responding following extinction has not been examined in mice with this model.
Based on findings that the GABAergic neurosteroid allopregnanolone (ALLO) significantly increased the consummatory phase of ethanol self-administration, the present study determined the effect of ALLO on the reinstatement of extinguished ethanol-seeking behavior and compared this effect to the reinstatement of responding for sucrose reward.
Materials and methods
Separate groups of male C57BL/6 mice were trained to lever press for access to a 10% ethanol (10E) or a 5% sucrose (5S) solution. A single response requirement of 16 presses (RR16) on an active lever resulted in 30 min of continuous access to the 10E or 5S solution. After the animals responded on the RR16 schedule for 14 weeks, mice were exposed to 30 min extinction sessions where responding had no scheduled consequence. Once responding stabilized below the preextinction baseline, mice received an intraperitoneal injection of ALLO (0, 3.2, 5.6, 10, or 17 mg/kg) 15 min prior to the extinction session in a within-subjects design.
ALLO produced a dose-dependent increase in responding under nonreinforced conditions in both the 10E and 5S groups. Additional work documented the ability of a conditioned cue light or a compound cue (light+lever retraction) to reinstate nonreinforced responding on the previously active lever.
These findings definitively show that conditioned cues and priming with ALLO are potent stimuli for reinstating both ethanol- and sucrose-seeking behavior in C57BL/6 mice.
KeywordsOperant self-administration GABA Conditioned cues Alcohol Relapse Reward
- Reinstatement of ethanol and sucrose seeking by the neurosteroid allopregnanolone in C57BL/6 mice
Volume 201, Issue 3 , pp 423-433
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- Operant self-administration
- Conditioned cues
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- Author Affiliations
- 1. VAMC Research (R&D-49), Department of Veterans Affairs Medical Center, 3710 SW U.S. Veterans Hospital Road, Portland, OR, 97239, USA
- 2. Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR, 97239, USA