Deletion of the beta 2 nicotinic acetylcholine receptor subunit alters development of tolerance to nicotine and eliminates receptor upregulation
- Sarah E. McCallumAffiliated withInstitute for Behavioral Genetics, University of ColoradoThe Parkinson's Institute Email author
- , Allan C. CollinsAffiliated withInstitute for Behavioral Genetics, University of Colorado
- , Richard PaylorAffiliated withDepartment of Molecular and Human Genetics, Baylor College of Medicine
- , Michael J. MarksAffiliated withInstitute for Behavioral Genetics, University of Colorado
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Chronic nicotine exposure induces both tolerance and upregulation of [3H]nicotine binding sites in rodent and human brain. However, the mechanism for chronic tolerance is unclear because a direct relationship between tolerance and receptor upregulation is not consistently observed.
In the present experiments, the role of β2* nicotinic acetylcholine receptors (nAChRs) on tolerance development and nAChR upregulation was examined following chronic nicotine treatment of β2 wild-type (+/+), heterozygous (+/−), and null mutant (−/−) mice.
Saline or nicotine (1, 2, or 4 mg/kg/h) was infused intravenously for 10 days. Locomotor activity and body temperature responses were measured before and after nicotine challenge injection to observe changes in nicotine sensitivity. [3H]Epibatidine binding was then measured in ten brain regions.
β2+/+ mice developed dose-dependent tolerance and upregulation of [3H]epibatidine binding sites. In contrast, β2−/− mice, initially less sensitive to acute nicotine's effects, became more sensitive following treatment with the lowest chronic dose (1 mg/kg/h). β2−/− mice treated with 4.0 mg/kg/h nicotine were no longer supersensitive, indicating that tolerance developed at this higher dose. However, these changes in nicotine sensitivity occurred in the absence of any nAChR changes in either low- or high-affinity [3H]epibatidine sites. Responses of β2+/− mice were intermediate between wild-type and mutant mice.
Upregulation of nAChRs in vivo requires the presence of the β2 subunit. Changes in nicotine sensitivity occurred both in the presence (β2+/+) and absence (β2−/−) of β2* nAChRs and suggest that mechanisms involving both β2* and non-β2* nAChR subtypes modulate adaptation to chronic nicotine exposure.
KeywordsNicotine β2 null mutant mouse Tolerance Sensitivity Receptor upregulation [3H]Epibatidine
- Deletion of the beta 2 nicotinic acetylcholine receptor subunit alters development of tolerance to nicotine and eliminates receptor upregulation
Volume 184, Issue 3-4 , pp 314-327
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- 1. Institute for Behavioral Genetics, University of Colorado, Boulder, CO, 80309, USA
- 3. The Parkinson's Institute, 1170 Morse Avenue, Sunnyvale, CA, 94089, USA
- 2. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, 77030, USA